| Project/Area Number |
16K19588
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Research Collaborator |
ONIZUKA Makoto 東海大学, 医学部, 准教授 (80366012)
SHINA Takashi 東海大学, 医学部, 教授 (00317744)
KIKKAWA Eri 東海大学, 医学部, 特定研究員
SUZUKI shingo 東海大学, 医学部, 特定研究員
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | HLA-LOH / 次世代シークエンサー / 同種移植後再発 / HLA不一致移植 / LOH / 同種造血幹細胞移植 / マイクロサテライト解析 / 次世代シーケンシング / SS-SBT法 / HLA遺伝子 / HLA / HLA片側アリル欠損 / 移植後再発 |
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| Outline of Final Research Achievements |
The loss of heterozygosity in HLA lesion has been reported in relapsed leukemic cell after allogeneic stem cell transplantation (Allo-HSCT). Because the patients who had relapsed their disease after Allo-HSCT were apparently worth prognosis, we investigated whether new method used SS-SBT method could detect the LOH or not and compared the conventional micro-satellite sequencing (MS) method. The detection rates were 33% in MS method, however, 100% in SS-SBT method. It seems that next generation sequencing method has high power of detection for HLA-LOH.
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