Analysis of aberrant methylation on CpG island of X chromosome in male schizophrenia
Project/Area Number |
16K19765
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Psychiatric science
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Research Institution | Kobe University |
Principal Investigator |
Otsuka Ikuo 神戸大学, 医学部附属病院, 助教 (40722880)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 統合失調症 / X染色体 / DNAメチル化 / 性差 |
Outline of Final Research Achievements |
Each CpG methylation status of CpG island A in 40 male schizophrenia (SCZ) patients and 40 healthy males (as "replication cohort") were analysed by MassARRAY; I revealed that approximately 30% of male SCZ have aberrant higher methylation of CpG island A. In addition, I investigated the association between TAF1 gene expression and methylation status of CpG island A using data from replication cohort. As in vitro study, I have been trying CRISPR-Cas experiments using male-derived neural stem cells and iPSC-derived neural cells in order to further clarify the biological importance of aberrant methylation of CpG island A.
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Academic Significance and Societal Importance of the Research Achievements |
統合失調症の有病率や重症度に性差があることはよく知られているが、その生物学的機序は不明であり、統合失調症とX染色体領域のメチル化異常に着目した研究はほとんどない。本解析を遂行できれば、世界に先駆けて統合失調症の病態や性差に関する生物学的異常の一部をDNAメチル化という可塑性(治療可能性)のある現象を通して解明することにつながり、統合失調症領域における今後の診断・治療の発展に大きな貢献ができると考える。
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Mitochondrial DNA copy number of peripheral blood in bipolar disorder: The present study and a meta-analysis.2018
Author(s)
Yamaki N, Otsuka I, Numata S, Yanagi M, Mouri K, Okazaki S, Boku S, Horai T, Ohmori T, Shirakawa O, Sora I, Hishimoto A.
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Journal Title
Psychiatry Research
Volume: 269
Pages: 115-117
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Longer telomeres in elderly schizophrenia are associated with long-term hospitalization in the Japanese population.2018
Author(s)
Zhang Y, Hishimoto A, Otsuka I, Watanabe Y, Numata S, Yamamori H, Boku S, Horai T, Someya T, Ohmori T, Hashimoto R, Sora I.
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Journal Title
J Psychiatr Res
Volume: 103
Pages: 161-166
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Increased serum levels and promoter polymorphisms of macrophage migration inhibitory factor in schizophrenia.2018
Author(s)
Okazaki S, Hishimoto A, Otsuka I, Watanabe Y, Numata S, Boku S, Shimmyo N, Kinoshita M, Inoue E, Ohmori T, Someya T, Sora I.
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Journal Title
Prog Neuropsychopharmacol Biol Psychiatry.
Volume: 83
Pages: 33-41
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Loss of chromosome Y in blood, but not in brain, of suicide completers2018
Author(s)
Kimura A, Hishimoto A, Otsuka I, Okazaki S, Boku S, Horai T, Izumi T, Takahashi M, Ueno Y, Shirakawa O, Sora I
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Journal Title
PLoS One
Volume: 13
Issue: 1
Pages: e0190667-e0190667
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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