Novel metastatic factors identified by proteomics using formalin fixed paraffin embedded tissue of pancreatic adenocarcinoma stroma

• Project/Area Number: 16K19914
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Tohoku University
• Principal Investigator: Takadate Tatsuyuki 東北大学, 高度教養教育・学生支援機構, 助教 (50772216)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵癌 / プロテオミクス / 間質 / 転移 / 質量分析 / 膵癌間質 / リンパ節転移 / プロテオーム

Outline of Final Research Achievements

Pancreatic ductal adenocarcinoma with lymph node metastasis (n=5) and without lymph node metastasis (n=5), resected in Tohoku University from 2005 to 2015, were analyzed. Stroma collected from FFPE tissue sections by laser microdissection were processed for LC-MS/MS. Fourteen candidate proteins were identified by semiquantitative comparison. To confirm the associations between candidate proteins and clinicopathological features, we immunohistochemically analyzed a cohort of 165 cases. It was suggested that HPX was associated with lymph node metatasis and vessel invasion.