Adipose-derived stromal cell transplantation for treatment of acute spinal cord injury

• Project/Area Number: 16K20043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: University of Fukui
• Principal Investigator: Takahashi Ai 福井大学, 学術研究院医学系部門(附属病院部), 助教 (90615303)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 急性脊髄損傷 / 脂肪由来間質細胞 / 細胞移植 / 骨髄間質細胞 / 脊髄損傷治療 / 遺伝子導入脂肪由来幹細 / 酸化ストレス / 遺伝子導入 / 脂肪由来幹細胞

Outline of Final Research Achievements

The use of mesenchymal stromal cell (MSC) transplantation to repair the injured spinal cord has shown consistent benefits in preclinical models. However, the low survival rate of grafted MSC is one of the most important problems.
In this study, we revealed these stromal cells exhibited a similar cell surface marker profile, but may express different levels of growth factors and cytokines in vitro. AD-MSCs were more proliferative with greater culture viability under these hypoxic conditions than BM-MSCs. AD-MSCs were also better able to tolerate oxidative stress conditions than BM-MSCs; similarly when transplanted into the spinal cord injury region in vivo, AD-MSCs demonstrated a higher survival rate post transplantation. Furthermore, this increased AD-MSC survival post transplantation was associated with preservation of axons and enhanced vascularization compared with the BM-MSC transplanted group.

Academic Significance and Societal Importance of the Research Achievements

脂肪由来間質細胞（AD-MSC）は比較的簡便に多量に採取可能であり、自家移植が可能な材料として有用性が報告されている。今回の研究では、骨髄間質細胞（BM-MSC）と比較した質的な優位性が明らかとなり、脊髄損傷に対する新たな治療法としての有用性が示唆された。

Research Products

• [Journal Article] Comparison of Mesenchymal Stromal Cells Isolated from Murine Adipose Tissue and Bone Marrow in the Treatment of Spinal Cord Injury. (2018)
  • Author(s): Takahashi A, Nakajima H, Uchida K, Takeura N, Honjoh K, Watanabe S, Kitade M, Kokubo Y, Johnson WEB, Matsumine A.
  • Journal Title: Cell Transplantation Volume: 27 Issue: 7 Pages: 39-47
  • DOI: 10.1177/0963689718780309
  • Peer Reviewed / Open Access
• [Journal Article] 脂肪由来間質細胞と骨髄間質細胞のストレス耐性比較および脊髄損傷に対する治療効果. (2018)
  • Author(s): 髙橋 藍, 中嶋秀明, 山本悠介, 小久保安朗, 松峯昭彦
  • Journal Title: Journal of Spine Research Volume: 9 Pages: 157-161
  • Peer Reviewed
• [Journal Article] 脂肪由来間質細胞による脊髄損傷治療. (2018)
  • Author(s): 高橋 藍, 中嶋秀明, 山本悠介, 松峯昭彦
  • Journal Title: 臨床整形外科 Volume: 53 Pages: 7-12