Elucidating the involvement of Scleraxis-positive progenitors during rotator cuff tendon-bone healing after acute injury in mice
| Project/Area Number |
16K20062
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
IDEO Katsumasa 熊本大学, 大学院医学教育部, 大学院生
YONEMITSU Ryuji 熊本大学, 大学院医学教育部, 大学院生
SHUKUNAMI Chisa 広島大学, 医歯薬保健学研究院(歯), 教授 (60303905)
HIRAKI Yuji 京都大学ウイルス, 再生医科学研究所, 教授 (40144498)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | Scleraxis / Sox9 / 腱板 / enthesis / tendon-bone healing / progenitor / scleraxis / 腱骨結合部 / 修復過程 |
|---|
| Outline of Final Research Achievements |
Improved understanding of the mechanisms underlying the healing process after rotator cuff (RC) tendon-bone site (enthesis) injury is critical to establish effective repair promotion after surgical RC repair. Recently, it was reported that a specific multipotent Scleraxis (Scx)-positive progenitors with a history of expressing Sox9, which gives rise to tenocytes and chondrocytes, contribute to enthesis development. In this study, we aimed to elucidate the involvement of Scx+ and Scx+/Sox9+ cells in a healing model of supraspinatus tendon enthesis following injury in mature ScxGFP transgenic mice. We showed that small numbers of Scx+ and Scx+/Sox9+ cells transiently emerged at the repair site, but failed to regenerate native fibrocartilaginous enthesis. Our findings suggest that a healing mechanism mediated by enthesis-related progenitors may be potentially equipped in adult mice, although it appears to be limited.
|
Report
(3 results)
Research Products
(3 results)
