| Project/Area Number |
16K20148
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Hiromu 札幌医科大学, 医学部・分子生物学講座, 教授 (20381254)
MASUMORI Naoya 札幌医科大学, 医学部・泌尿器科学講座, 教授 (20295356)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 膀胱癌 / マイクロRNA / 抗がん剤抵抗性 / シスプラチン抵抗性 / シスプラチン / マイクロＲＮＡ / メチレーション / 抗癌剤抵抗性 / GC療法 |
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| Outline of Final Research Achievements |
In present study, we aimed to unveil the mechanism of CDDP resistance in bladder cancer cells and focused on microRNA-200b. We established CDDP resistant T24 bladder cancer cells (T24RC) and compared to parental cells (T24). Among these two cell lines many microRNAs were changed dramatically. Over expression of microRNA200b, re-sensitize T24RC cells to CDDP. Moreover we confirmed that microRNA-200b is epigenetically regulated. When treated by 5-aza-2'-deoxycitidine, expression of microRNA-200b recovered and also sensitivity to CDDP recovered. Combination of CDDP and 5-aza-2'-deoxycytidine synergistically suppressed cell proliferation in T24RC cells.
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