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Elucidation of the relationship of mitochondrial dynamics in the axons and induction mechanism of apoptosis of reitinal ganglion cells

Research Project

Project/Area Number 16K20311
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionUniversity of Fukui

Principal Investigator

Miyake Seiji  福井大学, 学術研究院医学系部門, 助教 (50572765)

Research Collaborator INATANI MASARU  
OKI MASAYA  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords網膜神経節細胞 / 軸索輸送 / ミトコンドリア / 酸性顆粒 / 緑内障 / 酸化ストレス / サイトカイン / 軸索障害 / オートファジー / 細胞死 / オルガネラ
Outline of Final Research Achievements

In this study, effect of external stimulation of several stresses such as oxidative stress, cytokines, and excitatory amino acid on dynamics of axonal transport of retinal ganglion cells was evaluated. TNF-α and hydrogen peroxide caused axonal shortening and decline in number of mobile mitochondria, however, cell death was not induced by these stimulations in this experimental condition. On the other hands, NMDA and IL-6 had no effect on axonal dynamics and cell viability. Previous our study revealed that depression of axonal transport activity occurred prior to cell death. These data suggested cytokine and oxidative stress were contribute to the risk for progression of glaucomatous optic neuropathy, regardless of ocular pressure.

Academic Significance and Societal Importance of the Research Achievements

緑内障治療のポイントは網膜神経節細胞の消失を少しでも遅らせることである。しかし、手術や点眼を行っても患者によって得られる効果が一定ではないことから、医療現場ではこれまでとは異なるアプローチの治療法が強く望まれている。研究代表者が進める神経保護を指向した緑内障治療法の開発は、治療戦略の幅を広げることができ、視覚障害から生じる総合的なコストを減ずるための方策を、眼圧下降とは異なる視点から提案できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Effect of Microtubule Disruption on Dynamics of Acidic Organelles in the Axons of Primary Cultured Retinal Ganglion Cells2018

    • Author(s)
      Miyake Seiji、Takihara Yuji、Yokota Satoshi、Takamura Yoshihiro、Inatani Masaru
    • Journal Title

      Current Eye Research

      Volume: 43 Issue: 1 Pages: 77-83

    • DOI

      10.1080/02713683.2017.1370117

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of subconjunctival scarring after microincision vitrectomy surgery using 20-, 23-, 25- and 27-gauge systems in rabbits.2017

    • Author(s)
      Gozawa M, Takamura Y, Miyake S, Iwasaki K, Arimura S, Takihara Y, Inatani M.
    • Journal Title

      Acta Ophthalmol.

      Volume: 95 Issue: 7

    • DOI

      10.1111/aos.13459

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photocoagulation of the Retinal Nonperfusion Area Prevents the Expression of the Vascular Endothelial Growth Factor in an Animal Model.2017

    • Author(s)
      Gozawa M, Takamura Y, Miyake S, Matsumura T, Morioka M, Yamada Y, Inatani M.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 58 Issue: 13 Pages: 5946-5953

    • DOI

      10.1167/iovs.17-22739

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy.2017

    • Author(s)
      Takamura Y, Arimura S, Miyake S, Matsumura T, Gozawa M, Iwasaki K, Inatani M.
    • Journal Title

      J Ophthalmol.

      Volume: 2017 Pages: 8530261-8530261

    • DOI

      10.1155/2017/8530261

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 緑内障性視神経症における軸索障害2018

    • Author(s)
      三宅誠司
    • Organizer
      第122回日本眼科学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Effect of axonopathy on dynamics of acidic organelle in the axon of primary cultured retinal ganglion cells.2017

    • Author(s)
      Miyake S, Takihara Y, Yokota S, Takamura Y, Inatani M
    • Organizer
      Association for Research in Vision and Ophthalmology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 網膜神経節細胞の細胞死におけるオートファジーと軸索輸送障害との関係2017

    • Author(s)
      三宅誠司、瀧原祐史、横田聡、高村佳弘、稲谷大
    • Organizer
      第121回 日本眼科学会
    • Related Report
      2017 Research-status Report
  • [Presentation] HAT阻害剤を用いた糖尿病白内障予防薬の開発2017

    • Author(s)
      金田文人、高村佳弘、三宅誠司、稲谷大、内田博之、沖昌也
    • Organizer
      第11回 日本エピジェネティクス研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] エピジェネティックな発現制御を受ける糖尿病白内障発症メカニズムの解析2017

    • Author(s)
      金田文人、三宅誠司、高村佳弘、稲谷大、内田博之、沖昌也
    • Organizer
      第35回 北陸生化学会
    • Related Report
      2017 Research-status Report
  • [Presentation] エピジェネティクスに着目した糖尿病白内障予防と治療2017

    • Author(s)
      金田文人、高村佳弘、三宅誠司、稲谷大、内田博之、沖昌也
    • Organizer
      Beyond the Chromatin Club 第8回
    • Related Report
      2017 Research-status Report
  • [Presentation] Mechanism of Diabetic Cataract to Mediate Epigenetic Expression Regulation2017

    • Author(s)
      金田文人、高村佳弘、三宅誠司、稲谷大、内田博之、沖昌也
    • Organizer
      日本遺伝学会 第89回大会
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 白内障の予防剤および治療剤、並びに、これらを製造するための、DNA 損傷に応答するシグナル伝達経路を阻害する阻害剤の使用2017

    • Inventor(s)
      沖昌也、金田文人、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Holder
      沖昌也、金田文人、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 白内障の誘導方法、白内障のモデル生物、白内障の予防剤ならびに治療剤のスクリーニング方法、および、白内障誘導剤2017

    • Inventor(s)
      沖昌也、金田文人、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Holder
      沖昌也、金田文人、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 白内障の予防剤および治療剤、ならびに、これらを製造するためのHIF経路阻害剤の使用2017

    • Inventor(s)
      沖昌也、金田文人、釜田和馬、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Holder
      沖昌也、金田文人、釜田和馬、高村佳弘、三宅誠司、稲谷大、内田博之
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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