| Project/Area Number |
16K20349
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
Ueno Hitomi 国立研究開発法人国立成育医療研究センター, 小児血液・腫瘍研究部, 上級研究員 (30435630)
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| Research Collaborator |
Okita Hajime 慶応義塾大学, 医学部・病理学教室, 准教授
Takada Shuji 国立研究開発法人 国立成育医療研究センター, システム発生・再生医学研究部, 部長
Nakabayashi Kazuhiko 国立研究開発法人 国立成育医療研究センター, 周産期病態研究部・周産期ゲノミクス研究室, 室長
Kiyokowa Nobutaka 国立研究開発法人 国立成育医療研究センター, 小児血液・腫瘍研究部, 部長
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 腎明細胞肉腫 / BCOR / ポリコーム / 小児腎腫瘍 / ヒストン修飾 / 小児がん / 癌 / 小児 / エピゲノム |
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| Outline of Final Research Achievements |
Clear cell sarcoma of the kidney has a characteristic novel BCOR abnormality; i.e. duplicated sequences at important domain for polycomb repressive complex formation (BCOR internal tandem duplication: BCOR-ITD). We investigated binding ability of BCOR-ITD to BCOR binding proteins.
BCOR-ITD was capable to bind BCL6 and PCGF1, both of which are BCOR binding proteins. Therefore, BCOR-ITD could be involved in tumorigenesis by potential different mechanisms from BCOR loss of function mutations, such as frameshift and nonsense mutations. As a result of histone modification analysis of tumor samples using ChIP-sequencing, clear cell sarcoma of the kidney had some distinctive peak regions. The regions were also exhibited unique pattern in DNA methylation analysis.
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