Observation of fetal cutaneous regeneration using fibroblast subtype markers

• Project/Area Number: 16K20366
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: Suzuki Ayaka 慶應義塾大学, 医学部(信濃町), 助教 (80724062)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 皮膚 / 再生 / 線維芽細胞 / マーカー / 胎仔 / 再生医療

Outline of Final Research Achievements

As Ryan et al reported in 2014, there are different lineage of fibroblasts in murine skin. They reported the incorporation of these different fibroblast subtypes to the adult wound healing and hair regeneration, but the relationship between murine fetal skin regeneration after wounding and these fibroblasts subtypes have never been reported. We originally developed a new method to make wounding as early as embryonic day 13 (E13), and observed a relationship between skin regeneration and these fibroblast subtypes. We found that Lrig 1positive fibroblasts are key players which achieve cutaneous regeneration. But these characters diminished after they were cultured under two dimensional condition.