Establishment and pathomechanism clarifiant in metal allergy cross-reaction mouse model

• Project/Area Number: 16K20443
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tsurumi University
• Principal Investigator: Shigematsu Hiroaki 鶴見大学, 歯学部, 助教 (80737198)
• Research Collaborator: Hamada Yoshiki ; Suzuki Ryuji ; Kumagai Kenichi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 金属アレルギー / 交差反応 / マウスモデル / モデルマウス / 歯科用金属 / 歯科用菌属 / 免疫学 / 歯学 / アレルギー

Outline of Final Research Achievements

Metal allergy significantly deteriorates patients' QOL by causing intractable complications, such as palmoplantar pustulosis, and it has been awaited that metal allergy is pathologically understood and diagnostic methods are established. In this study, the metal allergy mouse model was employed for the detailed analysis of TCR in T cells which involved in the cross-reactivity among different metals, in order to elucidate antigen-specific immune responses of T cells involved in the development of allergy to dental materials composed of various metals. This study demonstrated the successful establishment of the metal allergy cross-reaction mouse model with appropriate pathology. In addition, we observed that allergen specific T cells used a specific TCR repertoire in metal allergy cross-reaction mouse model.

Academic Significance and Societal Importance of the Research Achievements

金属アレルギー患者は増加傾向にあり，難治性の合併症も引き起こし患者のQOLを妨げている．金属アレルギーの詳細な病態については，動物モデルが存在していなかったため，不明な点が多い，本研究では，ヒト金属アレルギーの病態に類似したマウスモデルを作製することに成功し，T細胞の受容体解析では，原因となる遺伝子情報の解析に成功することができた．本研究の成果は，新規診断・治療体系の確立の基盤となり，この分野の研究が加速されることが考えられる．本研究で得られた金属アレルギー交差反応に関わるT細胞の遺伝子情報が，科学的根拠に基づいた安全な歯科医療の確立に寄与することが予想される．

Research Products

• [Journal Article] Fexofenadine Suppresses Delayed-Type Hypersensitivity in the Murine Model of Palladium Allergy (2017)
  • Author(s): Matsubara Ryota、Kumagai Kenichi、Shigematsu Hiroaki、Kitaura Kazutaka、Nakasone Yasunari、Suzuki Satsuki、Hamada Yoshiki、Suzuki Ryuji
  • Journal Title: International Journal of Molecular Sciences Volume: 18 Issue: 7 Pages: 1357-1357
  • DOI: 10.3390/ijms18071357
  • Peer Reviewed / Open Access
• [Presentation] 金属アレルギー交差反応マウスモデルの作製と病態解明について (2018)
  • Author(s): 重松宏昭、熊谷賢一、松原陵太、仲宗根康成、鈴木隆二、濱田良樹
  • Organizer: 第63回公益社団法人日本口腔外科学会総会・学術大会