The molecular mechanisms of intra-cellular interaction in the trigeminal ganglion underlying ectopic pain associated with tooth pulp inflammation

• Project/Area Number: 16K20466
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: OHARA Kinuyo 日本大学, 歯学部, 専修医 (10731606)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯髄炎 / 異所性異常疼痛 / 三叉神経節細胞 / TLR4 / NFkB / MAPキナーゼ / 異所性疼痛 / NFｋB / 三叉神経節

Outline of Final Research Achievements

We have already reported that Hsp70 is expressed in the tooth pulp and transported to the trigeminal ganglion (TG)-cell bodies, extracellularly secreted from TG-cell bodies, and binds to TLR4 of TG neurons, which causes the enhancement of the excitability of TG neurons innervating the tongue. Based on the present study, following new findings were obtained: 1) TRPV1 expression was enhanced in the activated TG neurons via intracellular NFkB and MAPk cascades. 2) Also, some TG cells innervating both M1 and tongue were activated by tooth pulp inflammation, causing persistent pain in the tongue.

Research Products

• [Journal Article] Role of medullary astroglial glutamine synthesis in tooth pulp hypersensitivity associated with frequent masseter muscle contraction (2018)
  • Author(s): Watase Tetsuro、Shimizu Kohei、Ohara Kinuyo、Komiya Hiroki、Kanno Kohei、Hatori Keisuke、Noma Noboru、Honda Kuniya、Tsuboi Yoshiyuki、Katagiri Ayano、Shinoda Masamichi、Ogiso Bunnai、Iwata Koichi
  • Journal Title: Mol pain Volume: 14 Pages: 1-11
  • DOI: 10.1177/1744806918763270
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of transient receptor potential vanilloid 1 channel expression in orofacial cutaneous hypersensitivity following tooth pulp inflammation. (2018)
  • Author(s): Watase T, Shimizu K, Komiya H, Ohara K, Iwata K, Ogiso B.
  • Journal Title: J Oral Sci Volume: 60 Pages: 8-13
  • Peer Reviewed / Open Access
• [Journal Article] Phenotypic change in trigeminal ganglion neurons associated with satellite cell activation via extracellular signal-regulated kinase phosphorylation is involved in lingual neuropathic pain (2017)
  • Author(s): Mikuzuki Lou、Saito Hiroto、Katagiri Ayano、Okada Shinji、Sugawara Shiori、Kubo Asako、Ohara Kinuyo、Lee Jun、Toyofuku Akira、Iwata Koichi
  • Journal Title: European Journal of Neuroscience Volume: 46 Issue: 6 Pages: 2190-2202
  • DOI: 10.1111/ejn.13667
  • Peer Reviewed
• [Journal Article] Interaction between calcitonin gene-related peptide-immunoreactive neurons and satellite cells via P2Y(12) R in the trigeminal ganglion is involved in neuropathic tongue pain in rats. (2017)
  • Author(s): Sugawara S, Okada S, Katagiri A, Saito H, Suzuki T, Komiya H, Kanno K, Ohara K, Iinuma T, Toyofuku A, Iwata K.
  • Journal Title: Eur J Oral Sci. Volume: 125(6) Issue: 6 Pages: 444-452
  • DOI: 10.1111/eos.12382
  • Peer Reviewed
• [Journal Article] Ascending projections of nociceptive neurons from trigeminal subnucleus caudalis: A population approach. (2017)
  • Author(s): Saito H, Katagiri A, Okada S, Mikuzuki L, Kubo A, Suzuki T, Ohara K, Lee J, Gionhaku N, Iinuma T, Bereiter DA, Iwata K.
  • Journal Title: Experimantal Neurology Volume: 293 Pages: 124-136
  • DOI: 10.1016/j.expneurol.2017.03.024
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 急性歯髄炎により誘導される歯痛錯誤の末梢神経機構 (2017)
  • Author(s): 古宮 宏記, 清水 康平, 大原 絹代, 髙橋 なつみ, 小木曾 文内
  • Organizer: 日本歯科保存学会春季学術大会
• [Presentation] 急性歯髄炎により誘導される歯痛錯誤の末梢神経機構 (2016)
  • Author(s): 古宮 宏記, 清水 康平, 大原 絹代, 小林 寛, 會田 泰代, 小木曾 文内
  • Organizer: 日本歯科保存学会 2016 年度春季学術大会（第 144 回）
  • Place of Presentation: 栃木県総合文化センター(栃木県宇都宮市)
  • Year and Date: 2016-06-09
• [Book] 抜髄 Initial Treatment 治癒に導くための歯髄への臨床アプローチ (2016)
  • Author(s): 木ノ本喜史,興地隆史,大島勇人,澁川義幸,田崎雅和,村松敬,五十嵐勝,北島佳代子,新井恭子,松浦信幸,長谷川誠実,清水康平,羽鳥啓介,大原絹代,篠田雅路,小木曾文内,泉英之,阿部修,加藤広之,澤田則宏,佐藤暢也,岩波洋一,佐藤勧哉,北村和夫,吉川剛正,田中利典,石井宏,清水花織
  • Total Pages: 399
  • Publisher: ヒョーロン