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Investigating the mechanism of osteocytic mechanobiology using a gravity contoroller

Research Project

Project/Area Number 16K20511
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Prosthodontics/ Dental materials science and
Research InstitutionKyushu Dental College

Principal Investigator

Tamura Akiko  九州歯科大学, 歯学部, 助教 (30762067)

Research Collaborator IWANABE Yujiro  
KOBAYAKAWA Miki  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords骨細胞 / 微小重力 / メカノバイオロジー / 歯科
Outline of Final Research Achievements

We tried to culture IDG-SW3, bone cell line cells, which replicates osteoblast to late osteocyte differentiation in vitro, and primary osteocyte from mouse long bone, in a gravity controller, which can create microgravity environment. We wanted to know if it is possible to culture IDG-SW3 cells in microgravity environment.
We stopped the gravity controller every 12 hours and observed under microscope for 48 hours, continuously. Cell adhesion to the flask wall was intact in both the control and the experimental group and we could not find any differences in the survival of the cells throughout the time course.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-12-27  

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