Basic study of peri-implantitis treatment aimed at reosseointegration using hydroxyl radical

• Project/Area Number: 16K20531
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tohoku University
• Principal Investigator: Shirato Midori 東北大学, 歯学研究科, 助教 (60708501)
• Research Collaborator: NIWANO yoshimi ; NAKAMURA keisuke ; TENKUMO taichi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ヒドロキシルラジカル / 過酸化水素光分解 / インプラント周囲炎

Outline of Final Research Achievements

This study aimed to evaluate the capability of treatment based on hydroxyl radical for peri-implantitis. In the study, sandblasted titanium discs were used as specimens. Firstly, treated specimens were observed to investigate the effect of this treatment on a titanium surface. There were not significant differences between treated discs and　untreated discs. Then I analysed proliferation of mouse osteoblastic cells (MC3T3-E1) cultured on the treated discs. Compared with intact discs, biofilm (using Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis,　Fusobacterium nucleatum and Streptococcus mitis) contamination　lowered cell proliferation on the specimen surface,whereas this treatment　recovered cell proliferation.
These findings suggest that treatment based on hydroxyl radical can recover the degraded biocompatibility of biofilm-contaminated titanium surfaces without damage and can potentially be utilised for peri-implantitis treatment.

Academic Significance and Societal Importance of the Research Achievements

近年、インプラント治療は盛んに行われるようになった。その一方で、インプラント周囲炎もインプラント治療の普及に伴い増加の一途を辿っている。しかしながら、インプラント周囲炎の理想的な治療法は未だ確立しておらず、予後も良好とは言い難い。
本研究は、難しいとされているインプラント周囲炎治療に過酸化水素光分解殺菌というこれまでにない技術を取り入れることで、新しい適切な治療法として確立することを目的として行った。本治療法が臨床応用され、インプラント周囲炎の治療法として普及すれば、その難治性により苦しんでいる多くの人々のQOLを上げることができるため、非常に社会的意義が高い研究であると考える。

Research Products

• [Journal Article] Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation (2019)
  • Author(s): Nakamura K, Shirato M, Tenkumo T, Kanno T, Westerlund A, Ortengren U, Sasaki K, Niwano Y.
  • Journal Title: Sci Rep Volume: 9 Issue: 1 Pages: 4688-4688
  • DOI: 10.1038/s41598-019-41126-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Reconditioning of biofilm-contaminated titanium surface for osteoblast proliferation by hydroxyl radicals generated via H2O2 photolysis. (2018)
  • Author(s): Nakamura K, Shirato M, Tenkumo T, Kanno T, Westerlund A, Ortengren U, Sasaki K, Niwano Y.
  • Organizer: International Symposium for Multimodal Research and Education in IOHS-Liaison 2018 in Sendai
  • Int'l Joint Research