Investigation of the mechanisms of regulating tumor microenvironment by secreted micro RNA from intractable oral cancer, and development a new treatment strategy

• Project/Area Number: 16K20591
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University
• Principal Investigator: YOHSIDA RYOJI 熊本大学, 大学院生命科学研究部(医), 准教授 (10632458)
• Co-Investigator(Renkei-kenkyūsha): ITO Takaaki 熊本大学, 大学院生命科学研究部, 教授 (70168392) ; NAKAYAMA Hideki 熊本大学, 大学院生命科学研究部, 教授 (70381001)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔扁平上皮癌 / micro RNA / 腫瘍微小環境 / 循環miRNA / 治療抵抗性 / バイオマーカー / 口腔がん / エクソソーム / マイクロRNA / 放射線耐性 / 体液診断 / 歯学 / 癌 / 病理学 / トランスレーショナルリサーチ / マイクロアレイ

Outline of Final Research Achievements

The aim of this study is to investigate the mechanism of tumor microenvironment regulating secreted micro RNA from intractable oral cancer and development a new diagnosis/treatment strategy.
We compared the miRNA expression profile between the good-responder and poor-response for chemoradiotherapy by using plasma obtained from OSCC patient's. We found that the expression of miR-223、miR-659 and miR-1290 showed significant change among two groups. The expression status of these miRNA in plasma correlated with metastasis, recurrence and response to chemoradiotherapy of advanced OSCC. Moreover, the expression status of miRNA also correlated with patient's survival. These results suggest that circulating miRNA which is distinctive in intractable OSCC may be a promising target for new diagnostic modality and treatment strategy.

Research Products

• [Presentation] Investigation of the clinical significance of circulating miRNA-1290 in oral squamous cell carcinoma. (2017)
  • Author(s): Nakashima H, Yoshida R, Matsuoka Y, Kawahara K, Sakata J, Arita H, Nagata M, Hirosue A, Nakayama H, Shinohara M
  • Organizer: 第29回台湾口腔顎顔面外科学会年次総会・学術集会
  • Place of Presentation: aipei innovation city convention center、 台北市
  • Year and Date: 2017-03-04
• [Presentation] Investigation of the clinical significance of circulating miRNA-1290 in oral squamous cell carcinoma (2017)
  • Author(s): Hikaru Nakashima, Ryoji Yoshida, Yuichiro Matsuoka, Masashi Nagata, Akiyuki Hirosue, Kenta Kawahara,Jyunki Sakata, Hidetaka Arita, Akimitsu Hiraki, Masanori Shinohara and Hideki Nakayama
  • Organizer: Taiwanese Association of Oral and Maxillofacial Surgeons(TAOMS)
  • Int'l Joint Research
• [Presentation] Investigation of the clinical significance of circulating miRNA-1290 in oral squamous cell carcinoma. (2016)
  • Author(s): Hikaru Nakashima, Ryoji Yoshida, Yuichiro Matsuoka, Masashi Nagata, Akiyuki Hirosue, Kenta Kawahara,Jyunki Sakata, Hidetaka Arita, Akimitsu Hiraki and Hideki Nakayama
  • Organizer: 第75回日本癌学会学術大会
  • Place of Presentation: 横浜パシフィコ、神奈川
  • Year and Date: 2016-10-06
• [Presentation] 口腔扁平上皮癌患者における循環miRNAの発現に関する臨床的検討 (2016)
  • Author(s): 中嶋光, 吉田遼司, 廣末晃之, 有田英生, 坂田純基, 松岡祐一郎, 川原健太, 中川純泰, 永田将士, 中山秀樹
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: 大宮ソニックシティ、埼玉
  • Year and Date: 2016-06-09