Effects of the hardness and chemical composition in apical cementum on the occurrence of root resorption

• Project/Area Number: 16K20656
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: SHIMIZU Mami 日本大学, 松戸歯学部, 助手(専任扱) (50732856)
• Research Collaborator: UMEZAWA Eriko
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯根吸収 / セメント質 / エナメル質 / ビッカース硬さ / 硬度

Outline of Final Research Achievements

The purpose of this study was to investigate whether or not individual variation in the hardness and chemical composition of the cementum in the root apex affects the degree of root resorption.
Results: In pit formation assay, the resorbed area in the soft group showed a greater extent than the moderate and hard groups. A correlation was noted between the Vickers hardness and the resorbed area of the cementum in the root apex. The Ca/P ratio in the soft and moderate groups showed lower than the hard group. A correlation was noted between the Vickers hardness and the Ca/P ratio of the cementum in the root apex.
Conclusions: These results suggested that the hardness and Ca/P ratio of the cementum may be involved in the occurrence of root resorption caused by orthodontic forces.

Research Products

• [Journal Article] Daidzein inhibits relapse after rat experimental tooth movement. (2018)
  • Author(s): Erika Nakayama, Masaru Yamaguchi, Jun Kikuta, Mami Shimizu, Takuji Hikida, Momoko Hikida, Yoshiki Murakami, Masaaki Suemitu, Kayo Kuyama, Kazutaka Kasai.
  • Journal Title: International Journal of Oral-Medical Sciences Volume: -
  • NAID: 130007403287
  • Peer Reviewed / Open Access
• [Journal Article] Effects of daidzein on the production of type I collagen and matrix metalloproteinase1 by stretched human periodontal ligament cells. (2018)
  • Author(s): Masaru Yamaguchi, Erika Nakayama, Mami Shimizu, Jun Kikuta, Satoshi Horihata, Kazutaka Kasai.
  • Journal Title: International Journal of Oral-Medical Sciences Volume: -
  • NAID: 130007403289
  • Peer Reviewed / Open Access
• [Journal Article] Effect of caspases and RANKL induced by heavy force in orthodontic root resorption. (2018)
  • Author(s): Yukari Minato, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Takuji Hikida, Momoko Hikida, Masaaki Suemitsu, Kayo Kuyama, Kazutaka Kasai.
  • Journal Title: The Korean Journal of Orthodontics Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Micro-osteoperforations accelerate orthodontic tooth movement by stimulating periodontal ligament cell cycles. (2018)
  • Author(s): Tadasu Sugimori, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Takuji Hikida, Momoko Hikida, Yoshiki Murakami, Kazutaka Kasai.
  • Journal Title: American Journal of Orthodontics and Dentofacial Orthopedics Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Caspase-mediated Apoptosis by Compressive Force Induces RANKL in Cementoblasts. (2017)
  • Author(s): Masaru Yamaguchi, Yukari Minato, Mami Shimizu, Jun Kikuta, Takuji Hikida, and Kazutaka Kasai.
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 16(2) Pages: 31-38
  • NAID: 130006320938
  • Peer Reviewed / Open Access
• [Journal Article] Expressions of IL-34 in root resorption by excessive orthodontic force. (2017)
  • Author(s): 2.Kohno R, Yamaguchi M, Hikida T, Kikuta J, Shimizu M, Takahashi-Hikida M, Murakami Y, Kasai K.
  • Journal Title: International Journal of Oral-Medical Sciences. Volume: 印刷中
  • NAID: 130006894958
  • Peer Reviewed
• [Journal Article] An energy dispersive x-ray microanalysis study in root apex of human cementum. (2017)
  • Author(s): Yao-Umezawa E, Yamaguchi M, Shimizu M, Kikuta J, Suzuki K, Kasai K.
  • Journal Title: American Journal of Orthodontics and Dentofacial Orthopedics Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ラットの実験的歯の移動においてジグリングはIL-17を介して歯根吸収を重篤化する. (2017)
  • Author(s): 石川 友子，山口 大, 疋田 拓史，菊田 純，清水 真美，高橋 桃子，村上 嘉規，葛西 一貴.
  • Journal Title: 日大口腔科学 Volume: 印刷中
  • NAID: 40021289677
  • Peer Reviewed
• [Journal Article] Individual Variations in the Hardness and Elastic Modulus of the Human Cementum (2016)
  • Author(s): Yamaguchi M, Yao-Umezawa E, Tanimoto Y, Shimizu M, Kikuta J, Hikida T, Takahashi-Hikida M, Horihata S, Suzuki K, Kasai K.
  • Journal Title: Journal of Hard Tissue Biology Volume: 25 Issue: 4 Pages: 345-350
  • DOI: 10.2485/jhtb.25.345
  • NAID: 130005249139
  • ISSN: 1341-7649, 1880-828X
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comparison of orthodontic root resorption under heavy and jiggling forces during experimental tooth movement. (2016)
  • Author(s): Hikida T, Yamaguchi M, Shimizu M, Kikuta J, Yoshino T, Kasai K.
  • Journal Title: Korean J Orthod Volume: 46(4) Issue: 4 Pages: 228-241
  • DOI: 10.4041/kjod.2016.46.4.228
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Jiggling Force Aggravates Orthodontic Root Resorption Via TNF-α during Rat Experimental Tooth Movement (2016)
  • Author(s): Ohashi M, Yamaguchi M, Hikida T, Kikuta J, Shimizu M, Goseki T, Kasai K.
  • Journal Title: International Journal of Oral-Medical Sciences Volume: 14 Issue: 4 Pages: 82-90
  • DOI: 10.5466/ijoms.14.82
  • NAID: 130005142171
  • ISSN: 1347-9733, 2185-4254
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Relationship between root resorption and apoptosis in cementum. (2017)
  • Author(s): Yukari Minato, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Takuji Hikida, Momoko Hikida, Masaaki Suemitsu, Kayo Kuyama, Kazutaka Kasai
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Jiggling force aggregates root resorption via production of inflammatory cytokines (2017)
  • Author(s): Takuji Hikida, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Momoko Hikida, Kazutaka Kasai.
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Notch signaling aggravates root resorption by suppressing Wnt signaling (2017)
  • Author(s): Jun Kikuta, Masaru Yamaguchi, Mami Shimizu, Takuji Hikida, Momoko Hikida, Takehiro Iwane, Kazutaka Kasai
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Hardness and Ca/P ratio of cementum involve in root resorption (2017)
  • Author(s): Eriko Umezawa-Yao, Masaru Yamaguchi, Mami ShimizJun Kikuta, Kunihiro Suzuki, Kazutaka Kasai.
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Effect of nasal obstruction on the immune system (2017)
  • Author(s): Yoshiki Murakami, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Tomonori Sato, Yurie Horigome, Ryoki Kobayashi ,Kazutaka,Kasai
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Micro-osteoperforations accelerate orthodontic tooth movement by activating PDL cell cycles (2017)
  • Author(s): Tadasu Sugimori, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Takuji Hikida, Momoko Takahashi, Kazutaka Kasai.
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Compression Force Induces Wnt Signaling in Periodontal Ligament Cells (2017)
  • Author(s): Hitomi Saito, Masaru Yamaguchi, Mami Shimizu, Jun Kikuta, Yoshiki Murakami, Kazutaka Kasai.
  • Organizer: International Association for Dental Research（IADR）
  • Place of Presentation: Ｍｏｓｃｏｎｅ ｃｅｎｔｅｒ，San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Micro-osteoperforationsは歯槽骨の骨密度の減少を介して歯の移動速度を促進させる (2017)
  • Author(s): 杉森匡, 山口大, 清水真美, 菊田純, 疋田拓史, 疋田桃子, 村上嘉規, 葛西一貴
  • Organizer: 第76回日本矯正歯科学会（札幌）
• [Presentation] メカニカルストレスによる歯根膜のWntシグナル発現が、歯根吸収を増悪する (2017)
  • Author(s): 斎藤瞳, 山口大, 菊田純, 疋田拓史, 疋田桃子, 清水真美, 葛西一貴
  • Organizer: 第76回日本矯正歯科学会（札幌）
• [Presentation] 圧迫力負荷によるセメント芽細胞のアポトーシスと歯根吸収の関係性 (2017)
  • Author(s): 湊友香里, 山口大, 清水真美, 菊田純, 疋田拓史, 疋田桃子, 村上嘉規, 葛西一貴
  • Organizer: 第76回日本矯正歯科学会（札幌
• [Presentation] Daidzeinはコラーゲン代謝を活性化し後戻りを抑制する (2017)
  • Author(s): 中山瑛加, 山口大, 疋田拓史, 清水真美, 菊田純, 村上嘉規, 疋田桃子, 葛西一貴
  • Organizer: 第76回日本矯正歯科学会（札幌）
• [Presentation] ヒト歯根膜線維芽細胞の持続的圧迫力に対するNotchシグナルとWntシグナルの相互関係 (2017)
  • Author(s): 岩根健大, 山口大, 菊田純, 疋田拓史, 疋田桃子, 清水真美, 村上嘉規, 葛西一貴
  • Organizer: 第76回日本矯正歯科学会（札幌）
• [Presentation] 歯の移動による歯根吸収とセメント細胞のアポトーシスの関与 (2016)
  • Author(s): 湊友香里，山口大，清水真美，菊田純，高橋桃子，吉野智一，葛西一貴
  • Organizer: 第75回日本矯正歯科学会
  • Place of Presentation: アスティとくしま,徳島県, 徳島市
  • Year and Date: 2016-11-07
• [Presentation] 根尖部セメント質の物性および化学組成が歯根吸収の発生に及ぼす影響 (2016)
  • Author(s): 梅澤恵梨子，山口大，清水真美，吉野智一，菊田純，鈴木久仁博，葛西一貴
  • Organizer: 第75回日本矯正歯科学会
  • Place of Presentation: アスティとくしま,徳島県, 徳島市
  • Year and Date: 2016-11-07
• [Presentation] 歯の移動後のdaidzeinの後戻り抑制効果の検討 (2016)
  • Author(s): 中山瑛加，山口大，高橋桃子，疋田拓史，清水真美，菊田純，村上嘉規，吉野智一，葛西一貴
  • Organizer: 第75回日本矯正歯科学会
  • Place of Presentation: アスティとくしま,徳島県, 徳島市
  • Year and Date: 2016-11-07
• [Presentation] 持続的圧迫力がヒト歯根膜線維芽細胞のWnt5a発現に及ぼす影響について (2016)
  • Author(s): 斉藤瞳，山口大，菊田純，疋田拓史，高橋桃子，清水真美，村上嘉規，吉野智一，葛西一貴
  • Organizer: 第75回日本矯正歯科学会
  • Place of Presentation: アスティとくしま,徳島県, 徳島市
  • Year and Date: 2016-11-07
• [Presentation] Jiggling force aggravates orthodontic root resorption via TNF-α during rat experimental tooth movement (2016)
  • Author(s): Masaru Yamaguchi, Takuji Hikida, Tomokazu Yoshino, Jun Kikuta, Mami Shimizu, Momoko Takahashi, Kazutaka Kasai
  • Organizer: 38th Asia Pacific Dental Congress.APDC
  • Place of Presentation: Hong Kong Convention and Exhibition Centre, Hong Kong, China
  • Year and Date: 2016-06-17
• [Presentation] The lingual orthodontic treatment diagnosed in digital technology and analog setup model (2016)
  • Author(s): Katsuhiko Saito, Ryuichi Imamura, Shinichi Negishi, Mami Shimizu, Kazutaka Kasai
  • Organizer: The 1st Asian Lingual Orthodontic Meeting
  • Place of Presentation: Coex Conference Room South 4F, Seoul, Korea
  • Year and Date: 2016-04-09
  • Int'l Joint Research