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In vivo three-dimensional tissue analysis of periodontal tissue microvasculature induced to differentiate in coculture

Research Project

Project/Area Number 16K20679
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionThe Nippon Dental University

Principal Investigator

Shimizu Yutaka  日本歯科大学, 新潟生命歯学部, 助教 (10734717)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords歯周組織 / 微小血管 / 再生 / 脱分化脂肪細胞 / 再生療法 / 細胞 / 移植・再生医療
Outline of Final Research Achievements

In this study, rat dedifferentiated fat cells (RDFATs) were cocultured with rat gingival endothelial cells, and histological examination was performed to examine revascularization when cells were transplanted to periodontal tissue. Transplantation of cocultured RDFATs resulted in more neovascularization than did transplantation of RDFATs alone. Cocultured RDFATs also showed differentiation into blood vessels. Cocultured RDFATs are considered to be useful for wound healing and regeneration of periodontal tissue because they have high blood vessel-inducing ability and promote angiogenesis of defective tissue.

Academic Significance and Societal Importance of the Research Achievements

低侵襲かつ大量に取得できる脱分化脂肪細胞 (DFATs) は,歯肉由来血管内皮細胞 (GECs) との共培養により,歯周組織由来血管内皮細胞や歯周組織由来血管周皮細胞の特性に近い細胞に分化したと考えられる。またDFATsの移植は,血管新生や血流の改善を見込めることから,同様に歯肉毛細血管の機能を改善する可能性が考えられる。DFATsによる歯周組織血管内皮細胞および血管周皮細胞の再生および機能改善が可能であることが明らかとなれば,歯周組織の恒常性維持や歯周組織再生療法における血液,供給,創傷治癒において重要な役割を果たすと考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (1 results)

All 2017

All Presentation (1 results)

  • [Presentation] ラットを用いた脱分化脂肪細胞と歯肉由来血管内皮細胞の共培養による分化誘導の検討2017

    • Author(s)
      清水豊,丸山昂介,外山淳史,中島茂,佐藤聡
    • Organizer
      第60回春季歯周病学会学術大会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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