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Impact of commensal flora on periodontal immune response to lipopolysaccharide

Research Project

Project/Area Number 16K20694
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Social dentistry
Research InstitutionOkayama University

Principal Investigator

Irie Koichiro  岡山大学, 大学病院, 講師 (50509594)

Research Collaborator Fukuhara Daiki  
UCHIDA Yoko  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords口腔常在菌 / 歯周組織 / 免疫応答 / LPS / 歯周病 / 常在菌 / CD4+T細胞 / 歯学 / 免疫学 / 感染症
Outline of Final Research Achievements

Commensal flora are involved in the appropriate development of the mature immune system. However, it is unclear how commensal flora contribute to immune responses against lipopolysaccharide (LPS). Experimental groups received topical application of Porphyromonas gingivalis LPS (10 μg/μl) into the palatal gingival sulcus. Sampling was performed before LPS application (baseline) and at 3, 24, or 72 hours after LPS application. SPF mice, but not GF mice, showed an increased number of CD4+ T cells in the periodontal tissue at 3 hours after LPS application, compared with the number at baseline. Expression of genes encoding tumor necrosis factor-α, interleukin (IL)-17A, and IL-10 was also significantly higher in the SPF mice than in the GF mice at 3 hours after LPS application. LPS-exposed SPF mice exhibited increased CD4+ T cell migration and increased expression of inflammatory and anti-inflammatory cytokine genes in periodontal tissue compared to LPS-exposed GF mice.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (8 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] ワシントン大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Impact of commensal flora on periodontal immune response to lipopolysaccharide2018

    • Author(s)
      Fukuhara D, Irie K, Uchida Y, Kataoka K, Akiyama K, Ekuni D, Tomofuji T, Morita M
    • Journal Title

      Journal of Periodontology

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium.2017

    • Author(s)
      Irie K, Tomofuji T, Ekuni D, Fukuhara D, Uchida Y, Kataoka K, Kobayashi S, Kikuchi T, Mitani A, Shimazaki Y, Morita M.
    • Journal Title

      Arch Oral Biol

      Volume: 87 Pages: 72-78

    • DOI

      10.1016/j.archoralbio.2017.12.007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-Specific Neutrophil Migration and CXCL2 Expression in Periodontal Tissue2016

    • Author(s)
      Greer A, Irie K, Hashim A, Leroux BG, Chang AM, Curtis MA, Darveau RP
    • Journal Title

      Journal of Dental Research

      Volume: 印刷中 Issue: 8 Pages: 946-952

    • DOI

      10.1177/0022034516641036

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Impact of commensal bacteria on periodontal immune response against lipopolysaccharide2017

    • Author(s)
      Fukuhara D, Irie K, Uchida Y, Kataoka K, Ekuni D, Tomofuji T, Morita M
    • Organizer
      Penn Periodontal Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Commensal flora affects osteoblasts mineralization in mice2017

    • Author(s)
      Uchida Y, Irie K, Hattori T, Fukuhara D, Kataoka K, Ekuni D, Tomofuji T, Kubota S, Morita M
    • Organizer
      Penn Periodontal Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LPSを塗布した歯周組織の免疫応答に対する常在菌の影響2017

    • Author(s)
      福原大樹、入江浩一郎、内田瑶子、片岡広太、江國大輔、友藤孝明、森田学
    • Organizer
      第66回 日本口腔衛生学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 常在菌が骨芽細胞に与える影響について2017

    • Author(s)
      内田瑶子、入江浩一郎、福原大樹、片岡広太、江國大輔、友藤孝明、森田学
    • Organizer
      第66回 日本口腔衛生学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The impact of oral commensal flora on alveolar bone metabolism in mice2016

    • Author(s)
      Daiki Fukuhara, Koichiro Irie, Yoko Uchida, Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita
    • Organizer
      Innate Immunity in Host-Pathogen Interactions
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of aging on inflammatory response in periodontal tissue2016

    • Author(s)
      Daiki Fukuhara, Koichiro Irie, Yoko Uchida, Kota Kataoka, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita
    • Organizer
      Innate Immunity in Host-Pathogen Interactions
    • Place of Presentation
      Heidelberg, Germany
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 口腔常在菌が歯槽骨に与える影響について2016

    • Author(s)
      内田瑶子、入江浩一郎、福原大樹、片岡広太、江國大輔、友藤孝明、森田学
    • Organizer
      第65回日本口腔衛生学会・総会
    • Place of Presentation
      東京
    • Year and Date
      2016-05-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 歯周組織の加齢変化におけるCD4+ T細胞の影響2016

    • Author(s)
      福原大樹、入江浩一郎、内田瑶子、片岡広太、江國大輔、友藤孝明、森田学
    • Organizer
      第65回日本口腔衛生学会・総会
    • Place of Presentation
      東京
    • Year and Date
      2016-05-27
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-16  

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