| Project/Area Number |
16K20694
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Research Collaborator |
Fukuhara Daiki
UCHIDA Yoko
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔常在菌 / 歯周組織 / 免疫応答 / LPS / 歯周病 / 常在菌 / CD4+T細胞 / 歯学 / 免疫学 / 感染症 |
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| Outline of Final Research Achievements |
Commensal flora are involved in the appropriate development of the mature immune system. However, it is unclear how commensal flora contribute to immune responses against lipopolysaccharide (LPS). Experimental groups received topical application of Porphyromonas gingivalis LPS (10 μg/μl) into the palatal gingival sulcus. Sampling was performed before LPS application (baseline) and at 3, 24, or 72 hours after LPS application. SPF mice, but not GF mice, showed an increased number of CD4+ T cells in the periodontal tissue at 3 hours after LPS application, compared with the number at baseline. Expression of genes encoding tumor necrosis factor-α, interleukin (IL)-17A, and IL-10 was also significantly higher in the SPF mice than in the GF mice at 3 hours after LPS application. LPS-exposed SPF mice exhibited increased CD4+ T cell migration and increased expression of inflammatory and anti-inflammatory cytokine genes in periodontal tissue compared to LPS-exposed GF mice.
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