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Induction of autophagy via novel lysosomal localized Akt binding molecule

Research Project

Project/Area Number 16K20872
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Functional biochemistry
Research InstitutionHokkaido University

Principal Investigator

Noriyuki HIRATA  北海道大学, 遺伝子病制御研究所, 助教 (40595956)

Research Collaborator Noguchi Masayuki  
Suizu Futoshi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsオートファジー / PI3K-Akt-mTORシグナル伝達経路 / リソソーム / シグナル伝達 / 神経科学 / ウィルス / 感染症 / 免疫学 / PI3K-Akt-mTORシグナル伝達経路
Outline of Final Research Achievements

I identified VRK2 as the serine/threonine kinase binding lysosomal localized Akt. VRK2 is predicted control of induction of autophagy. In this study, I performed a research project aimed at the novel mechanism of autophagy induction via activity of Akt and VRK2 on lysosomal membrane. The index of autophagy induction (LC3 puncta, LC3II/I conversion and p62 reduction)in VRK2 shRNA-treated cells were reduced as compare to control cells. And also, I determined that the lysosomal enzymatic activity to protein degradation as the final step of autophagy is enhanced by VRK2.

Academic Significance and Societal Importance of the Research Achievements

本研究により、一般的にAktの下流のシグナルに存在するmTORC1が介するオートファジー誘導機構に、mTORC1を介さず、リソソーム膜上においてVRK2にAktが結合した複合体によりAkt活性を維持して誘導される機構が解明され、新たなオートファジー誘導の仕組みとして学術的な意義を持つと考える。また、この成果により、オートファジー異常の代表疾患、特に中枢神経系疾患であるアルツハイマー病、ハンチントン病、パーキンソン病に悩む患者に対する新たな治療法の開発につながることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2018 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Functional characterization of lysosomal interaction of Akt with VRK22018

    • Author(s)
      Hirata Noriyuki、Suizu Futoshi、Matsuda-Lennikov Mami、Tanaka Tsutomu、Edamura Tatsuma、Ishigaki Satoko、Donia Thoria、Lithanatudom Pathrapol、Obuse Chikashi、Iwanaga Toshihiko、Noguchi Masayuki
    • Journal Title

      Oncogene

      Volume: 37 Issue: 40 Pages: 5367-5386

    • DOI

      10.1038/s41388-018-0330-0

    • NAID

      120006540059

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Interaction of VRK2 with Akt at lysosomes controls induction of autophagy2018

    • Author(s)
      NOGUCHI Masayuki, HIRATA Noriyuki, SUIZU Futoshi
    • Organizer
      An AACR Special Conference on TARGETING PI3K/mTOR SIGNALING
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional characterization of lysosomal interaction of Akt with VRK22018

    • Author(s)
      HIRATA Noriyuki, SUIZU Futoshi , MATSUDA-LENNICOV Mami, TANAKA Tsutomu, EDAMURA Tatsuma, ISHIGAKI Satoko, DONIA Thoria, LITHANATUDOM Pathrapol, OBUSE Chikashi, IWANAGA Toshihiko, NOGUCHI Masayuki
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞膜リン脂質PI3Pの新規追跡技術の開発2018

    • Author(s)
      水津 太, 平田 徳幸, Thoria Donia, Bala Jyoti, 石垣 聡子, 野口 昌幸
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] リソゾームにおけるAKT-VRK2複合体におるオートファジー制御の仕組み2018

    • Author(s)
      野口 昌幸, 平田 徳幸, 水津 太
    • Organizer
      第11回オートファジー研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] リソソーム局在性Akt-VRK2複合体によるオートファジー誘導機構2018

    • Author(s)
      平田 徳幸, 水津 太, 野口 昌幸
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional characterization of lysosomal interaction of Akt with VRK22018

    • Author(s)
      HIRATA Noriyuki, SUIZU Futoshi , MATSUDA-LENNICOV Mami, TANAKA Tsutomu, EDAMURA Tatsuma, ISHIGAKI Satoko, DONIA Thoria, LITHANATUDOM Pathrapol, OBUSE Chikashi, IWANAGA Toshihiko, NOGUCHI Masayuki
    • Organizer
      The 37th Sapporo International Cancer Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of Akt with VRK2 at lysosomes controls induction of autophagy2018

    • Author(s)
      Hirata N, Suizu F, Matsuda-Lennikov M, Tanaka T, Edamura T, Ishigaki S, Donia T, Lithanatudom P, Obuse C, Iwanaga T, Noguchi M
    • Organizer
      第3回北大・部局横断シンポジウム 研究ネットワーク促進プログラム ~Young Researchers in Cutting-Edge Life Science~
    • Related Report
      2017 Research-status Report
  • [Presentation] 異なる結合因子によるAktの多様な生物機能2016

    • Author(s)
      野口昌幸、平田徳幸、水津太
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (神奈川県, 横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] Aktリン酸化依存的なInversin機能制御機構の解明2016

    • Author(s)
      水津太、平田徳幸、木村光輝、枝村達磨、田中努、石垣聡子、Thoria Donia、 野口寛子、岩永敏彦、野口昌幸
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (神奈川県, 横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Phosphorylation- dependent interaction of Akt and Inversin at basal body of primary cilia2016

    • Author(s)
      Suizu F, Hirata N, Kimura K, Edamura T, Tanaka T, Ishigaki S, Donia T, Noguchi H, Iwanaga T, Noguchi M
    • Organizer
      The 28th CDB Meeting, Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      RIKEN Center for Develpmental Biology (CDB), Kobe, Japan)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 一次繊毛におけるAKTとInversinとリン酸化依存的結合とその機能解析2016

    • Author(s)
      水津太、平田徳幸、田中努、野口昌幸
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (神奈川県, 横浜市)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] PI(3)P結合剤2018

    • Inventor(s)
      平田 徳幸
    • Industrial Property Rights Holder
      平田 徳幸
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Research-status Report
    • Overseas

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Published: 2016-04-21   Modified: 2020-03-30  

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