Glial control of neuronal excitability
Project/Area Number |
16K20917
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
Basic / Social brain science
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Research Institution | Tohoku University |
Principal Investigator |
Beppu Kaoru 東北大学, 医学系研究科, 非常勤講師 (40747458)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | アストロサイト / てんかん / 光遺伝学 / グリア細胞 |
Outline of Final Research Achievements |
Astrocytes release transmitters such as glutamate and lactate. We investigated how these transmitters are released from astrocytes. Excitatory neurons release glutamate and this glutamate activates glutamate transporters in astrocytes. Uptake of glutamate via glutamate transporters cause intracellular acidification in astrocytes. We found that ion flux and the resulting acidification trigger opening of volume-sensitive anion channels in astrocytes. Then glutamate anion is released through this channels. We also found that optogenetic alkalization of astocytes by photostimulation of archaerhodpsin in astrocytes enhanced extracellular lactate level. These results suggest that ion flux and the resulting pH fluctuation strongly regulates gliotransmitter release in astrocytes.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Targeted expression of step-function opsins in transgenic rats for optogenetic studies.2018
Author(s)
Hiroyuki Igarashi, Keiko Ikeda, Hiroshi Onimaru, Ryosuke Kaneko, Kyo Koizumi, Kaoru Beppu, Kayo Nishizawa, Yukari Takahashi, Fusao Kato, Ko Matsui, Kazuto Kobayashi, Yuchio Yanagawa, Shin-ichi Muramatsu, Toru Ishizuka, and Hiromu Yawo
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Journal Title
Scientific Reports
Volume: -
Related Report
Peer Reviewed / Open Access
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