Binding of ribosomal RNA genes to the nuclear periphery in budding yeast

• Project/Area Number: 16K20987
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: Horigome Chihiro 東京大学, 分子細胞生物学研究所, 助教 (10771206)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: リボソームRNA遺伝子 / 核膜 / 老化 / 微生物

Outline of Final Research Achievements

The ribosomal RNA genes (rDNA) in budding yeast are organized into a single tandem array of about 150 repeats. Fob1 unidirectionally inhibits replication fork progression at the replication fork barrier and induces DNA double-strand break (DSB) in the rDNA. The DSB leads to unequal sister-chromatid recombination and hence rDNA instability.
We performed chromatin immunoprecipitation assay to determine the localization of rDNA within the nucleus. We showed that rDNA binds to the nuclear pore in a manner dependent on Fob1 and DNA damage checkpoint kinase Tel1. The factors which are required for the rDNA-nuclear envelope binding are also important for the rDNA stability. We speculate that Fob1 sequesters rDNA at the nuclear periphery to inhibit aberrant recombination events.

Research Products

• [Presentation] リボソームRNA遺伝子はDNA複製阻害タンパク質Fob1に依存して核膜に結合する (2017)
  • Author(s): 堀籠智洋
  • Organizer: 第50回酵母遺伝学フォーラム
• [Presentation] Fob1-dependent binding of ribosomal RNA genes to the nuclear periphery in budding yeast (2017)
  • Author(s): 堀籠智洋
  • Organizer: 第55回日本生物物理学会年会
• [Presentation] Fob1-dependent binding of ribosomal RNA genes to the nuclear periphery in budding yeast (2016)
  • Author(s): Chihiro Horigome, Eri Unozawa and Takehiko Kobayashi
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 （神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Fob1-dependent binding of ribosomal RNA genes to the nuclear periphery in budding yeast (2016)
  • Author(s): Chihiro Horigome, Eri Unozawa and Takehiko Kobayashi
  • Organizer: 10th 3R International Symposium
  • Place of Presentation: ホテル一畑 （島根県・松江市）
  • Year and Date: 2016-11-13
  • Int'l Joint Research