Autophagy disruption by sugar chain in injury neurons

• Project/Area Number: 16K21083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience; Nerve anatomy/Neuropathology
• Research Institution: Nagoya University
• Principal Investigator: Ozaki Tomoya 名古屋大学, 医学系研究科, 助教 (40710588)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: コンドロイチン硫酸プロテオグリカン / 糖鎖 / dystrophic growth cone / オートファジー中断 / 軸索再生 / 脊髄損傷 / 軸索再伸長阻害 / オートファジー

Outline of Final Research Achievements

Our research analyzed hallmark of injury axon dystrophic growth cone and its formation. This study demonstrated that (i) autophagosomes accumulate in the dystrophic growth cone in vivo injury spinal cord of mouse, (ii) The formation of dystrophic growth cone was induced by gene knockdown of SNARE proteins which work at fusion step between autophagosomes and lysosomes in autophagic flux and (iii) chemically synthetized sugar chain or some compounds can rescue dystrophic growth cone cultured on chondroitin sulfate proteoglycan gradient. These result suggest autophagy as treatment target to axon regeneration failure.

Research Products

• [Int'l Joint Research] Academia Sinica(台湾)
• [Int'l Joint Research] Academia Sinica(台湾)
• [Presentation] Autophagy disruption and axon regeneration failure by chondroitin sulfate (2018)
  • Author(s): 尾崎智也、坂元一真、Yuanhao Gong、門松健治
  • Organizer: 第41回日本神経科学大会
• [Presentation] Sugar chain signals lead to autophagy disruption and axon regeneration failure (2017)
  • Author(s): 尾﨑 智也 、 坂元 一真 、 Yuanhao Gong、 内村 健治 、 門松 健治
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2017-07-20
• [Presentation] Autophagy disruption induced by sugar chain leads to axon regeneration failure (2017)
  • Author(s): Tomoya Ozaki, Kazuma Sakamoto, Yuanhao Gong, Kenji Uchimura, Kenji Kadomatsu
  • Organizer: 第81回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋市立 大学（愛知県名古屋市）
  • Year and Date: 2017-05-20
• [Presentation] Autophagy disryption induced by sygar chain leads to axon regeneration failure (2017)
  • Author(s): Tomoya Ozaki, Kazuma Sakamoto, Yuanhao Gong, Kenji Uchimura, Kenji Kadomatsu
  • Organizer: 第81回日本生化学会中部支部例会・シンポジウム
• [Presentation] Sugar chain signals lead to autophagy disruption and axon regeneration failure (2017)
  • Author(s): 尾崎智也、坂元一真、Yuanhao Gong、内村健治、門松健治
  • Organizer: 第40回日本神経科学大会
• [Presentation] 損傷した神経で起こる糖鎖によるオートファジー中断 (2016)
  • Author(s): 尾﨑 智也 、 坂元 一真 、 Yuanhao Gong、 内村 健治 、 門松 健治
  • Organizer: 第35回日本糖質学会年会
  • Place of Presentation: 高知市文化プラザ かるぽーと（高知県高知市）
  • Year and Date: 2016-09-01
• [Presentation] Chondroitin sulfate proteoglycan gradients disrupt autophagy and inhibit axon regeneration (2016)
  • Author(s): Tomoya Ozaki
  • Organizer: Toyama University International Symposium - Asian Network for Neuroscience -
  • Place of Presentation: 富山大学（富山県富山市）
  • Year and Date: 2016-07-20
  • Int'l Joint Research / Invited
• [Presentation] A proteoglycan gradient induce autophagy disruption and axon regeneration failure (2016)
  • Author(s): 尾﨑 智也 、 坂元 一真 、 Yuanhao Gong、 内村 健治 、 門松 健治
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20