The protective effects of fecal microbiota transplantation in a rat model of necrotizing enterocolitis.

• Project/Area Number: 16K21204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine; Bacteriology (including mycology)
• Research Institution: Kagawa University
• Principal Investigator: Fujii Takayuki 香川大学, 医学部, 助教 (00746696)
• Research Collaborator: Tanaka Aya ; Katami Hiroto ; Chiba Yoichi ; Ueno Masaki ; Imaohji Haruyuki ; Kuwahara Tomomi ; Onishi Shun ; Kaji Tatsuru ; Ieiri Satoshi ; Miki Takanori ; Shimono Ryuichi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 壊死性腸炎 / 短腸症候群 / Dysbiosis / 腸内細菌 / グアーガム / 腸内細菌叢 / guar gum / dysbiosis / 糞便移植 / 未熟児医学

Outline of Final Research Achievements

We investigated whether partially hydrolyzed guar gum ameliorates small intestinal mucosal damage and alters the intestinal microbiota using a rat small bowel resection (SBR) model.The mucosal degeneration rate was significantly higher in the SBR group. Partially hydrolyzed guar gum administration alleviated small intestinal mucosal damage, which could be associated with modulation of the intestinal microbiota.

Academic Significance and Societal Importance of the Research Achievements

新生児壊死性腸炎の重篤な合併症に短腸症候群がある。短腸症候群では腸管粘膜の萎縮や透過性の亢進から、腸内菌共生バランス失調を招き更なる合併症を引き起こすため、腸内環境の是正が重要である。食物繊維のグアーガムを用いることで小腸の粘膜が抑制され、腸内細菌叢の異常が是正される可能性を示した。このことは短腸症候群における新たな治療戦略の確立に寄与すると考えられる。

Research Products

• [Journal Article] Nutritional benefit of recycling bowel content in an infant with short bowel syndrome. (2017)
  • Author(s): Tanaka A, Nakayama-Imaohji H, Shimono R, Suzuki M, Fujii T, Kubo H, Yasuda S, Koyano K, Nakamura S, Katsuki N, Kuwahara T.
  • Journal Title: J Pediatr Gastroenterol Nutr Volume: 65 Issue: 3 Pages: 75-76
  • DOI: 10.1097/mpg.0000000000001630
  • Peer Reviewed / Open Access
• [Journal Article] Hypoxic-Ischemic Encephalopathy-Associated Liver Fatty Degeneration and the Effects of Therapeutic Hypothermia in Newborn Piglets. (2017)
  • Author(s): Kubo H, Shimono R, Nakamura S, Koyano K, Jinnai W, Yamato S, Yasuda S, Nakamura M, Tanaka A, Fujii T, Kanenishi K, Chiba Y, Miki T, Kusaka T, Ueno M.
  • Journal Title: Neonatology Volume: 111 Issue: 3 Pages: 203-210
  • DOI: 10.1159/000450721
  • Peer Reviewed / Open Access
• [Presentation] Partially hydrolyzed guar gum improves small intestinal damage in association with modulating intestinal microbiota following massive small bowel resection (2019)
  • Author(s): Takayuki Fujii, Yoichi Chiba, Haruyuki Nakayama-Imaohji, Shun Onishi, Aya Tanaka, Hiroto Katami, Tatsuru Kaji, Satoshi Ieiri, Takanori Miki, Masaki Ueno, Tomomi Kuwahara, Ryuichi ShimonoT
  • Organizer: 52nd Annual Scientific Meeting of the Pacific Association of Pediatric Surgeons
  • Int'l Joint Research
• [Presentation] 短腸症候群モデルにおけるグアーガムの腸管上皮保護作用と腸内細菌叢との関連 (2019)
  • Author(s): 藤井喬之，田中彩，形見祐人，上野正樹，今大路治之 ，桑原知巳，大西峻，加治健，家入里志，下野隆一
  • Organizer: 第119回日本外科学会学術集会
• [Presentation] 腸管機能不全児における腸内フローラ改善への取り組み (2017)
  • Author(s): 田中彩、下野隆一、藤井喬之、形見祐人、桑原知巳、今大路治之、鈴木基生
  • Organizer: 第117回日本外科学会定期学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2017-04-27