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Comprehensive analysis of ncRNA in blood for evaluation of fish health condition

Research Project

Project/Area Number 16K21208
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic bioproduction science
Aquatic life science
Research InstitutionEhime University

Principal Investigator

Shimizu Sonoko  愛媛大学, 南予水産研究センター, 准教授 (90531369)

Research Collaborator CHAKRABORTY Tapas  
TAKEUCHI Hisato  
KAWAKAMI Hidemasa  
MOHAPATRA Sipra  
HARAKAWA Shogo  
MATSUBARA Takahiro  
OHTA Kohei  
YOSHIHARA Yusaku  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsmiRNA / マダイ / 魚病 / 早期検出 / スモールRNA / エドワジエラ症 / ノンコーディングRNA / 水産学 / 魚病学 / 魚類生理学
Outline of Final Research Achievements

Edwardsiellois is a bacterial infection and causes mass motalitiy and quality deterioration of red seabream. Hence this disease become serious problem for aquaculture of Red seabream in Japan. To reduce the terible damages, it is important for early detection and counterpart for this disease. In this research, we comprehensively analyzed the micro RNA (miRNA) expressed in blood of infected and non-infected red seabream by NGS. In analysis using NGS, some miRNA significantly expressed in blood of infected fish in comparison with non-infected fish. Decrease of expression also showed in a few miRNA in infected fish. We also detected significant differences of some miRNA expression between non-infected and infected fish using quatative PCR methods. These results suggest that miRNA expression in blood of red seabream changes after infection of edwardsiellosis, and these miRNA may be useful as a molecular marker for early detection of infection with minimmal invasive diagnostic procedure.

Academic Significance and Societal Importance of the Research Achievements

我が国の魚類養殖において、魚病の発生は慢性化しており、安定的な養殖生産を行う上で大きな障害となっている。魚病による大量斃死や養殖魚の品質劣化を低減させるには、早期検出および早期対策が重要になる。本研究課題では、マダイの血液を用いて、マイクロRNA(miRNA)を網羅的に解析し、マダイ養殖で問題となっているエドワジエラ症感染時に発現が変化するmiRNAを明らかにした。本研究で得られたmiRNAのデータを分子マーカーとして用いることで、将来的には、魚類の血液を用いた簡便かつ定量的に魚病の早期発見や健康状態の把握が可能となり、被害の大幅軽減に繋がると期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge2018

    • Author(s)
      Noman Reza Mohammad Ali、Mohapatra Sipra、Shimizu Sonoko、Kitamura Shin-Ichi、Harakawa Shogo、Kawakami Hidemasa、Nakayama Kei、Sawayama Eitaro、Matsubara Takahiro、Ohta Kohei、Chakraborty Tapas
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 82 Pages: 286-295

    • DOI

      10.1016/j.fsi.2018.08.027

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major)2017

    • Author(s)
      Mohapatra Sipra、Chakraborty Tapas、Reza Mohammad Ali Noman、Shimizu Sonoko、Matsubara Takahiro、Ohta Kohei
    • Journal Title

      Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology

      Volume: 206 Pages: 42-53

    • DOI

      10.1016/j.cbpb.2017.01.009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] マダイのエドワジエラ症感染早期検出技術の開発2017

    • Author(s)
      松本真依・Sipra Mohapatra・Tapas Chakraborty・川上秀昌・原川翔悟・Reza Mohammad Ali Noman・太田耕平・松原孝博・清水園子
    • Organizer
      日本水産増殖学会第16回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Characterization of complement system association in stress responsive immunity modulation in red sea bream, Pagrus major2017

    • Author(s)
      Mohammad Ali Noman Reza, Tapas Chakraborty, Sipra Mohapatra, Shogo Harakawa, Hidemasa Kawakami, Takahiro Matsubara, Kohei Ohta, Sonoko Shimizu
    • Organizer
      日本水産増殖学会第16回大会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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