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The protective mechanism of an anti-nucleocapsid protein monoclonal antibody against influenza virus infections.

Research Project

Project/Area Number 16K21214
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Virology
Research InstitutionKagoshima University (2018)
Kyushu University (2016-2017)

Principal Investigator

Fujimoto Yoshikazu  鹿児島大学, 農水産獣医学域獣医学系, 准教授 (20613631)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsインフルエンザウイルス / トランスジェニックマウス / 抗体 / 感染抵抗性 / 感染防御抗体
Outline of Final Research Achievements

The purpose of this research subject was elucidation of antiviral mechanism of an anti-nucleocapsid protein (NP) antibody against influenza virus infections. It has been known that one of antiviral mechanisms of non-neutralizing antibodies is antibody-dependent cellular cytotoxicity (ADCC) and / or complement-dependent cellular cytotoxicity (CDC) activity. Transgenic (TG) mice expressing an anti-NP monoclonal antibody with mutations at several sites that are important for ADCC and CDC activities were generated, and experimental infections with an influenza virus were performed. As compared to wild type mice, TG mice expressing the ADCC / CDC activity-deficient antibody showed resistance to the viral infection. These results suggested that a different mechanism from ADCC and CDC activities may be responsible for protective effects of anti-NP antibodies against influenza virus infection.

Academic Significance and Societal Importance of the Research Achievements

将来発生する鳥インフルエンザの原因ウイルス亜型を予想することは困難である。亜型を問わず感染防御効果を示す万能ワクチンは、緊急時のウイルス感染拡大防止策の有効な手段となることや、罹患家禽と密に接する防疫従事者への感染予防に利用されることが期待される。ヌクレオカプシド蛋白質(NP)には、インフルエンザウイルスに共通した抗原領域が多く保存されているため、万能ワクチンの標的蛋白質として適しているが、その感染防御機序は明らかにはされてない。本研究成果は、抗NP抗体による感染防御機序に関する新たな知見を得られた点に学術的意義があり、NPを標的とした万能ワクチン開発に繋がる重要な知見であると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Antiviral effects against influenza A virus infection by a short hairpin RNA targeting the non-coding terminal region of the viral nucleoprotein gene2019

    • Author(s)
      Fujimoto Y, Kyogoku K, Takeda K, Ozaki K, Yamamoto S, Suyama H, Ono E.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 3 Pages: 383-388

    • DOI

      10.1292/jvms.18-0436

    • NAID

      130007614053

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cross-protective potential of anti-nucleoprotein human monoclonal antibodies against lethal influenza A virus infection2016

    • Author(s)
      Fujimoto Y., Tomioka Y., Takakuwa H., Uechi GI., Yabuta T., Ozaki K., Suyama H., Yamamoto S., Morimatsu M., Mai L.Q., Yamashiro T., Ito T., Otsuki K., Ono E.
    • Journal Title

      Journal of General Virology

      Volume: 97 Issue: 9 Pages: 2104-2116

    • DOI

      10.1099/jgv.0.000518

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] ヒト由来抗体発現トランスジェニックマウスを利用した抗NPモノクローナル抗体におけるインフルエンザウイルス感染防御能の解析2016

    • Author(s)
      藤本佳万、尾崎絹代、上地玄一郎、高桑弘樹、富岡幸子、藪田淑予、陶山晴香、山本沙代、森松正美、伊藤壽啓、大槻公一、Le Quynh Mai、山城哲、小野悦郎
    • Organizer
      第34回九州実験動物研究会
    • Place of Presentation
      産業医科大学(北九州市)
    • Year and Date
      2016-10-29
    • Related Report
      2016 Research-status Report
  • [Presentation] Anti-nucleocapsid protein antibody is sufficient to confer resistance to lethal infection with influenza A viruses of several subtypes in transgenic mice2016

    • Author(s)
      Fujimoto Y., Tomioka Y., Takakuwa H., Uechi GI., Yabuta T., Ozaki K., Suyama H., Yamamoto S., Morimatsu M., Mai L.Q., Yamashiro T., Ito T., Otsuki K., Ono E.
    • Organizer
      International Symposium for Southern China Laboratory Animal Sciences
    • Place of Presentation
      中華人民共和国・広東省
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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