| Project/Area Number |
16K21243
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
General medical chemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Research Collaborator |
NISHINO Koichiro
YASUDA Masahiro
LOVELAND Kate
ARAI Yoshikazu
TAKASAWA Ken
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 多能性幹細胞 / FGF / Nodal / Cripto / DNAメチル化 / ヒトiPS細胞 / Nodal-Activin / BMP / 幹細胞 / 再生医学 |
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| Outline of Final Research Achievements |
Human induced pluripotent stem (iPS) cells requires fibroblast growth factor (FGF) to maintain its proliferation and stemness. To clarify the role of FGF, analysis of gene expression kinetics of human iPS cells after FGF signal inhibition was performed. The results showed that disfunction of Cripto, which is essential for maintenance of human iPS cells, occurred before down-regulation of Cripto, suggesting that FGF maintains activation of Cripto via posttranscriptional regulation. We also revealed that Cripto gene expression is regulated by DNA methylation, but the down-regulation of Cripto after differentiation of human iPS cells did not involve DNA methylation.
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