| Project/Area Number |
16K21283
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
General anatomy (including histology/embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Katsutoshi Taguchi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (60462701)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | α-Synuclein / prion-like propagation / seed / Parkinson's disease / プリオン様伝播モデル / α-シヌクレイン / Fibril / Seed構造 / パーキンソン病 / Lewy bodies / Seed / Cell-cell transmission / alpha-Synuclein / neurodegeneration / toxic oligomer |
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| Outline of Final Research Achievements |
α-Synuclein (αSyn) is one of major components of Lewy bodies and Lewy Neurites, the well-known pathological hallmarks of synucleinopathies including Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). According to recent studies, cell-to-cell transmission of pathological αSyn in the brain is critical event for neurodegeneration. However, biochemical properties of these pathological molecules remain to be elucidated. Here, we isolated some characteristic αSyn-oligomers derived from preformed fibrils of αSyn. Next, we further recovered extracellular αSyn-oligomers having an uniform molecular mass in Native-PAGE from pathogenic conditioned culture medium. These oligomers successfully induced LB-pathology in cultured hippocampal neurons. Now, we are characterizing the pathogenic species of αSyn. These molecules will be a better target to inhibit the disease propagation via cell-to-cell transmission in PD and DLB.
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