Analysis of spatio-temporal functions and regulatory mechanisms of DREB2A, a drought- and heat-inducible transcription factor in Arabidopsis thaliana

• Project/Area Number: 16K21626
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: SATO Hikaru 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (90756058)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 植物 / シロイヌナズナ / 環境ストレス / 乾燥 / 高温 / 転写因子 / 転写複合体 / トランスクリプトーム解析 / 植物分子生理学 / 細胞種特異的制御 / INTACT / 乾燥ストレス / 高温ストレス / 転写制御 / 遺伝子発現制御

Outline of Final Research Achievements

A transcription factor DREB2A that is induced by drought and heat stress specifically regulates its target genes according to environmental conditions, however it remains unclear how the transcription factor specifically functions under drought and heat stress conditions. In the present study, we have identified two novel proteins that regulate DREB2A-target genes specifically under drought and heat stress conditions. They are specifically induced by drought or heat stress, respectively. They are suggested to form drought or heat stress-specific transcriptional complexes with DREB2A and activate DREB2A-target genes specifically under drought or heat stress conditions.

Academic Significance and Societal Importance of the Research Achievements

シロイヌナズナにおいてDREB2Aという因子は乾燥と高温ストレスの両方の応答を制御している。この因子に着目することで、乾燥と高温ストレスの2つの環境ストレスが細胞内でどのように別々に認識され、その応答が制御されているかという新たな知見を得られた。また、地球温暖化で引き起こされる干ばつや高温などの異常気象に対しても高い耐性を持つ作物の育種に役立つ情報を得ることができた。

Research Products

• [Journal Article] BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis (2017)
  • Author(s): Morimoto K, Ohama N, Kidokoro S, Mizoi J, Takahashi F, Todaka D, Mogami J, Sato H, Qin F, Kim JS, Fukao Y, Fujiwara M, Shinozaki K, Yamaguchi-Shinozaki K.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114 Issue: 40
  • DOI: 10.1073/pnas.1704189114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transcriptional Regulatory Network of Plant Heat Stress Response. (2017)
  • Author(s): Naohiko Ohama, Hikaru Sato, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki
  • Journal Title: Trends in Plant Science Volume: 1 Issue: 1 Pages: 53-65
  • DOI: 10.1016/j.tplants.2016.08.015
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] NF-YB2 and NF-YB3 have specific functions in response to drought and heat stress in cooperation with DREB2A in Arabidopsis thaliana (2017)
  • Author(s): Hikaru Sato, Takamasa Suzuki, Kazuo Shiniozaki, Kazuko Yamaguchi-Shinozaki
  • Organizer: Plant Genome Evolution 2017
  • Int'l Joint Research
• [Presentation] Enhancement of heat stress tolerance in rice by overexpressing Arabidopsis transcriptional regulator DPB3-1/NF-YC10 (2016)
  • Author(s): Hikaru Sato, Daisuke Todaka, Madoka Kudo, Junya Mizoi, Satoshi Kidokoro, Yu Zhao, Kazuo Shinozaki1 and Kazuko Yamaguchi-Shinozaki
  • Organizer: 27th International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research
• [Book] Survival Strategies in Extreme Cold and Desiccation (Chapter 12: Regulatory Gene Networks in Drought Stress Responses and Resistance in Plants) (2018)
  • Author(s): Fuminori Takahashi, Takashi Kuromori, Hikaru Sato, Kazuo Shinozaki
  • Publisher: Springer
• [Remarks] イネの高温ストレス耐性を向上する方法の開発
  • URL: http://www.a.u-tokyo.ac.jp/topics/2016/20160204-1.html
• [Remarks] 植物に乾燥・高温耐性を付与する転写因子DREB2Aが活性化する仕組みを解明
  • URL: http://www.a.u-tokyo.ac.jp/topics/2017/20170919-1.html