Analysis of autoimmune diseases pathogenesis with a novel reporter system
| Project/Area Number |
16KT0114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund |
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| Section | 特設分野 |
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| Research Field |
Complex Systems Disease Theory
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| Research Institution | National Center for Global Health and Medicine |
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Principal Investigator |
Sekiya Takashi 国立研究開発法人国立国際医療研究センター, その他部局等, 免疫応答修飾研究室長 (80519207)
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| Project Period (FY) |
2016-07-19 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 免疫学 / 制御性T細胞 / 生体イメージング / CD4T細胞 / in vivoレポーター / 自己免疫疾患 / T細胞 / レポーターシステム / 免疫寛容 / アレルギー・ぜんそく |
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| Outline of Final Research Achievements |
Helper T cells (Th) and regulatory T cells (Treg) play important roles in preventing false immune responses and activating the correct immune response depending on the type of antigen. It can be said that research on Th and Treg in the early stage of differentiation was delayed, but one of the main reasons for this is thought to be that a powerful system for detecting Th and Treg in the early stage of differentiation in vivo has not been established.
Therefore, in this study, we constructed a new reporter system that can monitor Th and Treg at the differentiation stage in vivo minimally invasively and can be isolated and analyzed as living cells, and we analyzed the pathogenesis of human multiple sclerosis mouse model EAE. By the analysis describing above, We succeeded in capturing the activation of the immune response during the course of EAE pathogenesis.
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| Academic Significance and Societal Importance of the Research Achievements |
分化初期段階のThやTregの研究は遅れていたと言えるが、本研究により、分化初期段階にあるThやTregをin vitro, in vivoの両方で検出できる強力な実験システムを構築することに成功した。このレポーターマウスは将来的に多くの研究組織にdistributeされ、様々な疾患を対象に解析されることで、炎症性疾患のみならず、多くの疾患の発症機構の解明、ひいては新規治療法の開発に寄与されると期待される。さらに本研究は、ヒト多発性硬化症マウスモデルEAEの解析により、既知の免疫応答のみならず、腸管や口腔など、解析の進んでいない部位からの免疫応答を検出しており、今後の研究展開に期待が持たれる。
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Generation of alloantigen-specific induced-Treg stabilized by vitamin C treatment and its application for prevention of acute graft versus host disease model2017
Author(s)
Hidenori Kasahara, Taisuke Kondo, Hiroko Nakatsukasa, Shunsuke Chikuma, Minako Ito, Makoto Ando, Yutaka Kurebayashi, Takashi Sekiya, Taketo Yamada, Shinichiro Okamoto, Akihiko Yoshimura
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Journal Title
International Immunology
Volume: 29
Issue: 10
Pages: 457-469
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Notch-mediated conversion of activated T cells into stem cell memory-like T cells for adoptive immunotherapy2017
Author(s)
Taisuke Kondo, Rimpei Morita, Yuumi Okuzono, Hiroko Nakatsukasa, Takashi Sekiya, Shunsuke Chikuma, Takashi Shichita, Mitsuhiro Kanamori, Masato Kubo, Keiko Koga, Takahiro Miyazaki, Yoshiaki Kassai, Akihiko Yohimura
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 15338-15338
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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