| Project/Area Number |
17076013
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MAZDA Osam Kyoto Prefectural University of Medicine, 医学研究科, 准教授 (00271164)
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| Co-Investigator(Kenkyū-buntansha) |
IMANISHI Jiro 京都府立医科大学, 医学研究科, 教授 (40112510)
FUSHIKI Shinnji 京都府立医科大学, 医学研究科, 教授 (80150572)
NAKAYA Takaaki 大阪大学, 微生物病研究所, 特任准教授 (80271633)
岸田 綱郎 京都府立医科大学, 医学研究科, 博士研究員 (00370205)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥101,000,000 (Direct Cost: ¥101,000,000)
Fiscal Year 2009: ¥19,700,000 (Direct Cost: ¥19,700,000)
Fiscal Year 2008: ¥20,600,000 (Direct Cost: ¥20,600,000)
Fiscal Year 2007: ¥20,600,000 (Direct Cost: ¥20,600,000)
Fiscal Year 2006: ¥20,200,000 (Direct Cost: ¥20,200,000)
Fiscal Year 2005: ¥19,900,000 (Direct Cost: ¥19,900,000)
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| Keywords | 幹細胞 / 細胞情報・動態 / 遺伝子発現 / 再生医学 / サイトカイン / アレルギー / 腫瘍免疫 / エピゲノム制御 / 遺伝子導入 / 遺伝子改変 / マルチスケール操作 / 免疫療法 / 再生医療 / 遺伝子 / 発現制御 / 細胞・組織 / ナノバイオ / 生体機能利用 / システム細胞工学 |
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| Research Abstract |
Gene transfection provides a fundamental technology to analyze, estimate and manipulate cellular and biomolecular functions. The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and oriP sequence offer characteristics of an artificial chromosome, drastically improving transduction rate as well as expression level in mammalian cells. Using multiscale manipulation technologies, we analyzed intracellular localization, interaction, replication and maintenance of EBNA1/oriP in living cells, and applied them to regulation of gene expression as well as to regenerative medicine.
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