Channel micro-assembly in junctional membrane complexes

• Project/Area Number: 17081001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2006-2009); Tohoku University (2005)
• Principal Investigator: TAKESHIMA Hiroshi Kyoto University, 薬学研究科, 教授 (70212024)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥69,400,000 (Direct Cost: ¥69,400,000); Fiscal Year 2009: ¥14,700,000 (Direct Cost: ¥14,700,000); Fiscal Year 2008: ¥14,700,000 (Direct Cost: ¥14,700,000); Fiscal Year 2007: ¥14,700,000 (Direct Cost: ¥14,700,000); Fiscal Year 2006: ¥18,800,000 (Direct Cost: ¥18,800,000); Fiscal Year 2005: ¥6,500,000 (Direct Cost: ¥6,500,000)
• Keywords: ジャンクトフィリン / 結合膜構造 / 小胞体Ca^<2+>放出 / 小胞体 / 膜修復 / ミツグミン53 / TRICチャネル / 肺胞上皮細胞 / 筋ジストロフィー / RBCCファミリー / TRIMファミリー / Ca2+放出 / リアノジン受容体 / Ca2+結合タンパク質 / 神経可塑性 / 後科分極 / カウンターイオン / 後過分極 / カルシウムストア / ノックアウトマウス / Ca2+チャネル

Research Abstract

The aim of this project is to understand the molecular basis of channel crosstalk in junctional membrane complexes between the plasma membrane and the endo-/sarcoplasmic reticulum. In particular, this project focused on i) biological roles of junctophilin subtypess as proteins producing junctional membrane complexes, ii) physiological abnormalities observed in knockout mice lacking junctophilins, iii) human diseases caused by mutated junctophilins, and iv) the new mechanisms supporting the junctophilin-mediated channel crosstalk.

Research Products

• [Journal Article] Essential role of TRIC-B channel in Ca^<2+>-handling of alveolar epithelium and perinatal lung maturation. (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R., Takeshima, H.
  • Journal Title: Development 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] (*co-corresponding authors) MG53 nucleates assembly of cell membrane repair machinery. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H.*, Ma, J.*
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] Mitsugumin 53-mediated maintenance of K+ currents in cardiac myocytes. (2009)
  • Author(s): Masumiya.H., Asaumi. Y., Nishi, M., Minamisawa.S., Adachi. Akahane. S., Yoshida, M., Kangawa, K., Ito, K., Kagaya, Y., Yanagisawa, T., Yamazaki, T., Ma, J., Takeshima, H.
  • Journal Title: Channels 3 Pages: 6-11
  • Peer Reviewed
• [Journal Article] Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolon-3 and (2009)
  • Author(s): Cai, C., Weisleder.N., Ko, J.K., Komazaki, S.,Sunada. Y.,Nishi.M., Takeshima, H, Ma.J.
  • Journal Title: J.Biol.Chem. 284 Pages: 15894-15902
  • Peer Reviewed
• [Journal Article] Essentlal role of TRIC-B channel ln Ca2+-handllng of alveolar eplthellum and perlnatalhlng (2009)
  • Author(s): Yamazaki. D., Komazaki. S., Nakanishi. H., Mishima. A., Nishi. M., Yazawa. M.,Yamazaki. T., Taguchi, R.Takeshuma. H.
  • Journal Title: Develpment 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] MG53 nucleates assembly of cell membrane repair machinery (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, Hwang, M., Ko, J-K., Lin, P., Thornton, A., Zhao, X. , Pan, Z., Komazaki, S., Brotto, M., Takeshima, H. & Ma, J.
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] MG53 regulates membrane budding and exocytosis in muscle cells (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Pan, Z., Nishi, M., Ko, J-K., Komazaki, S., Takeshima, H. & and Ma, J.
  • Journal Title: J. Biol. Chem 284 Pages: 3314-3322
  • Peer Reviewed
• [Journal Article] Genetic evidence for functional role of ryanodine receptor 1 in pulmonary artery smooth muscle cells (2009)
  • Author(s): Li, X. Q., Zheng, Y. M., Rathore, R., Ha, J., Takeshima, H. & Wang, Y. X
  • Journal Title: Pflugers Arch. 457 Pages: 771-783
  • Peer Reviewed
• [Journal Article] Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP. (2008)
  • Author(s): Stewart, R., Song, L., Carter, S.M., Sigalas, C., Zaccai, N.R., Kanamarlapudi, V., Bhat, M.B., Takeshima, H., Sitsapesan, R.
  • Journal Title: J. Membr. Biol. 222 Pages: 65-77
  • Peer Reviewed
• [Journal Article] Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. (2007)
  • Author(s): Ikeda, A., Miyazaki, T., Kakizawac, S., Okuno, Y., Tsuchiya, S., Myomoto, A., Saito, S., Yamamoto, T., Yamazaki, T., Iino, M., Tsujimoto, G., Watanabe, M., Takeshima, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 363 Pages: 835-839
  • Peer Reviewed
• [Journal Article] TRIC channels are essential for Ca^<2+> handling in intracellular stores. (2007)
  • Author(s): Yazawa, M., Ferrante, C., Feng, J., Mio, K., Ogura, T., Zhang, M., Lin, P-H., Pan, Z., Komazaki, S., Kato, K., Nishi, M., Zhao, X., Weisleder, N., Sato, C., Ma, J., Takeshima, H.
  • Journal Title: Nature 448 Pages: 78-82
  • Peer Reviewed
• [Journal Article] Mutations of junctophilin type 2 associated with hypertrophic cardiomyopathy. (2007)
  • Author(s): Matsushita, Y., Furukawa, T., Kasanuki, H., Nishibatake, M., Kurihara, Y., Ikeda, A., Kamatani, N., Takeshima, H., Matsuoka, R.
  • Journal Title: J. Hum. Genet. 52 Pages: 543-548
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca^<2+>-binding transmembrane protein on the endoplasmic reticulum. (2007)
  • Author(s): Zhang, M., Yamazaki, T., Yazawa, M., Treves, S., Nishi, M., Murai, M., Shibata, E., Zorzato, F., Takeshima, H.
  • Journal Title: Cell Calcium 42 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. (2007)
  • Author(s): Kakizawa, S., Kishimoto, Y., Hashimoto, K., Miyazaki, T., Furutani, K., Shimizu, H., Fukaya, M., Nishi, M., Sakagami, H., Ikeda, A., Kondo, H., Kano, M., Watanabe, M., Iino, M., Takeshima, H.
  • Journal Title: EMBO J. 26 Pages: 1924-1933
  • Peer Reviewed
• [Journal Article] Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. (2007)
  • Author(s): Kakizawa, S., Kishimoto, Y., Hashimoto, K., Miyazaki, T.,
  • Journal Title: EMBO J. 26 Pages: 1927-1933
  • Peer Reviewed
• [Journal Article] Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. (2007)
  • Author(s): Ikeda, A., Miyazaki, T., Kakizawac, S., Okuno, Y.,
  • Journal Title: Biochem. Biophys. Res. Commun. 363 Pages: 835-839
  • Peer Reviewed
• [Journal Article] TRIC channels are essential for Ca2+ handling in intracellular stores. (2007)
  • Author(s): Yazawa, M., Ferrante, C., Feng, J., Mio, K., Ogura, T., Zhang,
  • Journal Title: Nature 448 Pages: 78-82
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca2+-binding transmembrane protein on the endoplasmic reticulum. (2007)
  • Author(s): Zhang, M., Yamazaki, T., Yazawa, M., Treves, S., Nishi, M., Murai
  • Journal Title: Cell Calcium (in press)
• [Journal Article] Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. (2007)
  • Author(s): Kakizawa, S., Kishimoto, Y., Hashimoto, K., Miyazaki, T.
  • Journal Title: EMBO J. (in press)
• [Journal Article] Uncoupling of store-operated Ca^<2+> entry and altered Ca^<2+> release from sarcoplasmic reticulum through silencing of junctophilins. (2006)
  • Author(s): Hirata, Y., Brotto, M., Weisleder, N., Chu, Y., Lin, P., Zhao, X., Thornton, A., Komazaki, S., Takeshima, H., Ma, J., Pan, Z.
  • Journal Title: Biophys. J. 90 Pages: 4418-4427
  • Peer Reviewed
• [Journal Article] Functional uncoupling between Ca^<2+> release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins. (2006)
  • Author(s): Moriguchi, S., Nishi, M., Komazaki, S., Sakagami, H., Miyazaki, T., Masumiya, H., Saito, S., Watanabe, M., Kondo, H., Yawo, H., Fukunaga, K., Takeshima, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 10811-10816
  • Peer Reviewed
• [Journal Article] Muscle aging is associated with compromised Ca2+ spark signaling and segregated intracellular Ca2+ release. (2006)
  • Author(s): Weisleder, N., Brotto, M., Komazaki, S., Pan, Z., Zhao, X.
  • Journal Title: J.Cell Biol. 174 Pages: 639-645
• [Journal Article] Methamphetamine sensitization in nociceptin receptor knockout mice : locomotor and c-fos expression. (2005)
  • Author(s): Okabe ほか3名
  • Journal Title: Eur.J.Pharmacol. 507 Pages: 57-67
• [Journal Article] Impaired Ca^<2+> store functions in skeletal and cardiac muscle cells from sarcalumenin-deficient mice. (2005)
  • Author(s): Yoshida ほか11名
  • Journal Title: J.Biol.Chem. 280 Pages: 3500-3506
• [Journal Article] Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells. (2005)
  • Author(s): Matsuya ほか11名
  • Journal Title: J.Neurochem. 93 Pages: 674-685
• [Journal Article] Enhanced hippocampal acetylcholine release in nociceptin receptor knockout mice. (2005)
  • Author(s): Umezu ほか9名
  • Journal Title: Brain Res. 1050 Pages: 118-123
• [Journal Article] Defective place cell activity in nociceptin receptor knockout mice with elevated NMDA receptor-dependent long-term potentiation. (2005)
  • Author(s): Taverna ほか9名
  • Journal Title: J.Physiol. 565 Pages: 579-591
• [Journal Article] Enhanced resistance to fatigue and altered calcium handling properties of sarcalumenin knockout mice. (2005)
  • Author(s): Zhao ほか8名
  • Journal Title: Physiol.Genomics 23 Pages: 72-78
• [Presentation] Immuno-proteomic approach to E-C coupling: junctophilin and TRIC channel in cardiac Ca^<2+> release (2009)
  • Author(s): Takeshima, H.
  • Organizer: IUPS Whole-day Symposium
  • Place of Presentation: in Kyoto
  • Year and Date: 2009-07-28
• [Presentation] TRIC channel subtypes and SR/ER Ca^<2+> release. (2009)
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference; Muscle Excitation-Contraction Coupling
  • Place of Presentation: in Waterville Valley, USA
  • Year and Date: 2009-06-18
• [Presentation] Bichemical and biophysical characterization of mitsugumin 33 (2008)
  • Author(s): Younis, N., Okuda, K., Nishi, M., Yamazaki, T., Aoki, S., Carter,
  • Organizer: Biophysics Society Meeting
  • Place of Presentation: Long Beach, California
  • Year and Date: 2008-02-04
• [Presentation] Ryanodine receptor-mediated Ca^<2+> release from sarcoplasmic reticulum (2006)
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference; Muscle Excitation-Contraction Coupling
  • Place of Presentation: in New London, USA
  • Year and Date: 2006-06-09
• [Presentation] Mitsugumins, a series of novel membrane proteins in skeletal muscle (2006)
  • Author(s): Takeshima, H.
  • Organizer: Muscle Symposium in Basel
  • Place of Presentation: in Basel, Switzerland
  • Year and Date: 2006-05-05
• [Presentation] Junctophilin-mediated channel coupling during muscle E-C coupling (2005)
  • Author(s): Takeshima, H.
  • Organizer: European Muscle Conference
  • Place of Presentation: in Debrecen, Hungary
  • Year and Date: 2005-09-17
• [Book] Junctophilin. Encyclopedia of movement disorders. (2009)
  • Author(s): Takeshima, H.
  • Publisher: Elsevier Limitted
• [Book] 医歯薬系学生のための基礎生命科学 (2008)
  • Author(s): 竹島浩、山崎哲男、池田篤史、輿水崇鏡
  • Total Pages: 214
  • Publisher: 京都廣川書店
• [Book] イオンチャネル最前線(リアノジン受容体, 医学のあゆみ別冊) (2005)
  • Author(s): 竹島浩
• [Book] イオンチャネル最前線(医学のあゆみ別冊),pp.171-175担当 (2005)
  • Author(s): 竹島 浩
  • Total Pages: 273
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/