Development of diagnosis and treatment method of man sterility based on analyses of spermatogenesis-assaciatal genes

• Project/Area Number: 17209052
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: TANAKA Hiromitsu (2006-2007) Osaka University, Research Institute for Microbial Diseases, Assistant Professor (10263310); 西宗 義武 (2005) 大阪大学, 微生物病研究所, 招聘教授 (80029793)
• Co-Investigator(Kenkyū-buntansha): OKUYAMA Akihiko Osaka University, Graduate School of Medicine, Professor (20093388) ; TSUJIMURA Akira Osaka University, Graduate School of Medicine, Associate Professor (40294053) ; MIYAGAWA Yasushi Osaka University, Graduate School of Medicine, Assistant Professor (70362704) ; 田中 宏光 大阪大学, 微生物病研究所, 助手 (10263310)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥49,530,000 (Direct Cost: ¥38,100,000、Indirect Cost: ¥11,430,000); Fiscal Year 2007: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2006: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2005: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
• Keywords: Testis / Sperm / male infertilit / Development / gene / protein / Specific / Treatment / 男性不妊症 / SNP

Research Abstract

As many as 15% of couples are unable to conceive, and males are known to be responsible for half of the instances of infertility. Although advances in medical technology have allowed some infertile couples to have children, the causes of infertility are, in most cases, unknown. Many genes are expressed exclusively in the testes, particularly in the testicular germ cells. It is reasonable to propose that functional deficiencies in the genes expressed exclusively in spermatogenesis should lead to male infertility when there is no observable abnormality in the somatic cells of various organs. Hence, in order to understand idiopathic male infertility, development of diagnosis and treatment method of male infertility based on analyses of spermatogenesis-associated genes is essential. In this study, we found infertility specific protein in human semen and identified polymorphisms associated with male infertility in human haploid germ cell specific genes.

Research Products

• [Journal Article] Isolation and Characterization of the Haploid Germ Cell-Specific Smrpl Gene for a Novel Manchette Protein in the Mouse. (2008)
  • Author(s): Matsuoka Y., et. al.(他11名)
  • Journal Title: Mol. Reprod. Dev. 75 Pages: 967-975
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Taip2 is a novel cell death-related gene expressed in the brain during development. (2008)
  • Author(s): Yamada K., et. al.(他6)
  • Journal Title: Biochem. Biophys. Res. Commun. 369 Pages: 426-431
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Relationship between the substances in seminal plasma and the Acrobeads test[○!R]. (2008)
  • Author(s): Komori K., et. al.(他8)
  • Journal Title: Fertil. & Steril. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nitric Oxide synthesis leads to Vascular Endothelial Growth Factor Synthesis via the NO/Cyclic Guanosine 3', 5'-Monophosphate(cGMP)Pathway in Human Corpus Cavernosal Smooth Muscle Cells. (2008)
  • Author(s): Komori K., et. al.(他7)
  • Journal Title: J. Sex. Med. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Androgen Decline in Patients with Nonobstructive Azoospemia After Microdissection Testicular Sperm Extraction. (2008)
  • Author(s): Takada S., et. al.(他12)
  • Journal Title: Urology. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Meichroacidin containing the MORN motif is essential for spermatozoa morphogenesis. (2008)
  • Author(s): Tokuhiro K., et. al.(他16)
  • Journal Title: J Biol Chem. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation and Characterization of the Haploid Germ Cell-Specific Smrp1 Gene for a Novel Manchette Protein in the Mouse (2008)
  • Author(s): Matsuoka, Y, Miyagawa, Y, Tokuhiro, K, Kitamura, K, Iguchi, N, Maekawa, M, Takahashi, T, Tsujimura, A, Matsumiya, K, Okuyama, A, Nishimune, Y, Tanaka, H
  • Journal Title: Mol Reprod Dev 75 Pages: 967-975
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Taip2 is a novel cell death-related gene expressed in the brain during development (2008)
  • Author(s): Yamada, K, Akiyama, N, Yamada, S, Tanaka, H, Saito, S, Hiraoka, M, Kizaka-Kondoh, S
  • Journal Title: Biochem Biophys Res Commun 369 Pages: 426-431
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Taip2 is a novel cell death-related gene expressed in the brain during development. (2008)
  • Author(s): Yamada K, 他6名
  • Journal Title: Biochem Biophys Res Commun (In press)
  • Peer Reviewed
• [Journal Article] Isolation and characterization of the spermatid-specific Smrpl gene encoding a novel manchette protein. (2008)
  • Author(s): Matsuoka M, 他11名
  • Journal Title: Mol Reprod Dev. (In press)
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of gene expression in testes producing haploid germ cell susing DNA microarray analysis. (2007)
  • Author(s): Ike A., et. al.(他5)
  • Journal Title: Int. J. Androl. 30 Pages: 462-475
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The 193-Base Pair Gsg2(Haspin)Promoter Region Regulates Germ Cell-Specific Expression Bidirectionally and Synchronously. (2007)
  • Author(s): Tbkuhiro k., et. al.(他6)
  • Journal Title: Biol. Reprod. 76 Pages: 407-414
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Production of Functional Spematids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules. (2007)
  • Author(s): Kita K., et. al.(他14)
  • Journal Title: Biol. Reprod. 76 Pages: 211-217
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Prostatic stem cell marker identified by cDNA microarray in mouse. (2007)
  • Author(s): Tsujimura A., et. al.(他7)
  • Journal Title: J. Urol. 178 Pages: 686-691
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential brain processing of audiovisual sexual stimuli in men: comparative positron emission tomography study of the iniitiation and maintenance of penile erection during sexual arousal. (2007)
  • Author(s): Miyagawa Y., et. al.(他13)
  • Journal Title: Neuroimage 36 Pages: 830-842
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Clinical comparison of successful and failed microdissection TESE in patients with non-mosaic Klinefelter syndrome. (2007)
  • Author(s): Koga M., et. al.(他11)
  • Journal Title: Urology 70 Pages: 341-345
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Decreased number of mast cells infiltrating into needle biopsy specimens leads to a better prognosis of prostate cancer. (2007)
  • Author(s): Nonomura N., et. al.(他9)
  • Journal Title: Br. J. Cancer 97 Pages: 952-956
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of gene expression in testes producing haploid germ cells using DNA microarray analysis (2007)
  • Author(s): Ike, A, Tokuhiro, K, Hirose, M, Nozaki, M, Nishimune, Y, Tanaka, H
  • Journal Title: Int J Androl 30 Pages: 462-475
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The 193-Base Pair Gsg2 (Haspin) Promoter Region Regulates Germ Cell-Specific Expression Bidirectionally and Synchronously (2007)
  • Author(s): Tokuhiro, K, Miyagawa, Y, Yamada, S, Hirose, M, Ohta, H, Nishimune, Y, Tanaka, H
  • Journal Title: Biol Reprod 76 Pages: 407-414
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules (2007)
  • Author(s): Kita, K, Watanabe, T, Obsaka, K, Hayashi, H, Kubota, Y, Nagashima, Y, Aoki, I, Taniguchi, H, Noce, T, Inoue, K, Mild, H, Ogonuki, N, Tanaka, H, Ogura, A, Ogawa, T
  • Journal Title: Biol Reprod 76 Pages: 211-217
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prostatic stem cell marker identified by cDNA microarray in mouse (2007)
  • Author(s): Tsujimura, A Fujita, K, Takao, T, Miyagawa, Y, Takada, S, Matsumiya, K, Nonomura, N, Okuyama, A
  • Journal Title: J. Urol 178 Pages: 686-691
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential brain processing of audiovisual sexual stimuli in men : comparative positron emission tomography study of the initiation and maintenance of penile erection during sexual arousal (2007)
  • Author(s): Miyagawa, Y, Tsujimura, A, Fujita, K, Matsuoka, Y, Takahashi, T, Takao, Ti, Takada, S, Matsumiya, K, Osaki, U, Takasawa, M, Oku, M, Hatazawa, J, Kaneko, S, Okuyama, A
  • Journal Title: Neuroimage 36 Pages: 830-842
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical comparison of successful and failed microdissection TESE in patients with non-mosaic Klinefelter syndrome (2007)
  • Author(s): Koga, M, Tsujimura, A., Takeyama, A, Kiuchi, H, Takao, T, Miyagawa, Y, Takada, S, Matsumiya, K, Fujioka, H, Okamoto, Y, Nonomura, N, Okuyama, A
  • Journal Title: Urology 70 Pages: 341-345
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Decreased number of mast cells infiltrating into needle biopsy specimens leads to a better prognosis of prostate cancer (2007)
  • Author(s): Nonomura, N, Takayama, H, Nishimura, K, Oka, D, Nakai, Y, Shiba, M, Tsujimura, A, Nakayama, M, Aozasa, K, Okuyama, A
  • Journal Title: Br. J. Cancer 97 Pages: 952-956
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nitric Oxide synthesis leads to Vascular Endothelial Growth Factor Synthesis via the NO/Cyclic Guanosine 3',5'-Monophosphate (cGMP) Pathway in Human Corpus Cavernosal Smooth Muscle Cells (2007)
  • Author(s): Komori, K., Tsujimura, A., Takao, T., Matsuoka, Y., Miyagawa, Y., Takada, S., Nonomura, N., Okuyama, A
  • Journal Title: J. Sex. Med, Biol Reprod 76(in press) Pages: 407-414
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular biological features of male germ cell differentiation (2007)
  • Author(s): Tanaka, H, Hirose, M, Tokuhiro, K, Tainaka, H, Miyagawa, Y, Tsujimura, A, Okuyama, A, Nishimune, Y
  • Journal Title: Reprod Med Biol 6 Pages: 1-9
  • NAID: 10018849469
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single nucleotide polymorphisms : discovery of the genetic causes of male infertility (2007)
  • Author(s): Tanaka, H, Hirose, M, Tokuhiro, K, Matsuoka, Y, Miyagawa, Y, Tsujimura, A, Okuyama, A, Nishimune, Y
  • Journal Title: Soc Reprod Fertil Suppl 65 Pages: 531-534
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Microdissection testicular sperm extraction : prediction, outcome, and complications (2007)
  • Author(s): Tsujimura, A
  • Journal Title: Int J Urol 14 Pages: 883-889
  • NAID: 10019947540
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single nucleotide polymorphisms: discovery of the genetic causes of male infertility. (2007)
  • Author(s): Tanaka H, 他7名
  • Journal Title: Soc Reprod Fertil Suppl. 65 Pages: 531-534
  • Peer Reviewed
• [Journal Article] Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules. (2007)
  • Author(s): Kita K, 他14名
  • Journal Title: Biol. Reprod. 76 Pages: 211-217
  • Peer Reviewed
• [Journal Article] The 193-base pair Gsg2 (haspin) promoter region regulates germ cell-specific expression bidirectionally and synchronously. (2007)
  • Author(s): Tokuhiro K, 他6名
  • Journal Title: Biol. Reprod. 76 Pages: 407-414
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of gene expression in testes producing haploid germ cells using DNA microarray analysis. (2007)
  • Author(s): Ike A, 他5名
  • Journal Title: Int J Androl 30 Pages: 462-475
  • Peer Reviewed
• [Journal Article] Molecular biological features of male germ cell differentiation (2007)
  • Author(s): Tanaka H, 他7名
  • Journal Title: Reprod Med Biol 6 Pages: 1-9
  • NAID: 10018849469
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of gene expression in testes producing haploid germ cells using DNA microarray analysis (2007)
  • Author(s): Ike Akiko 他 5名
  • Journal Title: International Journal of Andrology (in press)
• [Journal Article] The 193-Base Pair Gsg2 (Haspin) Promoter Region Regulates Germ Cell-Specific Expression Bidirectionally and Synchronously. (2007)
  • Author(s): Tokuhiro keizo 他 6名
  • Journal Title: Biology Reproduction 76 Pages: 407-414
• [Journal Article] Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules. (2007)
  • Author(s): Kita Kaoru 他 14名
  • Journal Title: Biology Reproduction 76 Pages: 211-217
• [Journal Article] Laparoscopic Nephroureterectomy for Upper Tract Transitional Cell Carcinoma : Comparison of Laparoscopic and Open Surgery (2006)
  • Author(s): Tsujihata, M, Nonomura, N, Tsujimura, A, Yoshimura, K, Miyagawa, Y, Okuyama, A
  • Journal Title: Eur. Urol 49 Pages: 332-336
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein (2006)
  • Author(s): Tanaka, H, Matsuoka, Y, Onishi, M, Kitamura, K, Miyagawa, Y, Nishimura, H, Tsujimura, A, Okuyama, A, Nishimure, Y
  • Journal Title: Int. J. Androl 29 Pages: 353-359
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Laparoscopic adrenalectomy for primary hyperaldosteronism : clinical experience with 60 cases (2006)
  • Author(s): Tsujihata, M, Nonomura, N, Tsujimura, A, Nishimura, K, Yoshimura, K, Okuyama, A
  • Journal Title: J. Endourol 20 Pages: 262-265
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inter/intra-variation in measurements of testicular volumes by ten investigators from five institutions (2006)
  • Author(s): Tatsunami, S, Itoh, N, Nishida, T, Miyano, S, Matsumiya, K, Tsujimura, A, Eguchi, J, Takehara, K, Ko, E, Maeda, Y, Sasao, T, Tabata, C, Iwamoto, T
  • Journal Title: Asian J. Androl 8 Pages: 373-378
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A case of renal cell carcinoma with multiple lung metastasis refractory to interferon-a showing complete remission by interleukin-2 monotherapy (2006)
  • Author(s): Hayashi, T, Miyagawa, Y, Tsujimura, A, Nonomura, N, Minami, M, Okuyama, A
  • Journal Title: Int. J. Urol 13 Pages: 805-808
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative Study of Sperm Motility Analysis System[○!R] (SMAS[○!R]) and Conventional Microscopic Semen Analysis (2006)
  • Author(s): Komori, K, Tsujimura, A, Ishijima, S, Tanjapatku, P, Fujita, K, Matsuoka, Y, Takao, T, Miyagawa, Y, Takada, S, Okuyama, A
  • Journal Title: Reprod. Med. & Biol 5 Pages: 195-200
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficacy and safety of tadalafil 5, 10, and 20mg in Japanese men with erectile dysfunction : Results of a Multicenter, Randomized, Double-Blind, Placebo-Controlled Study (2006)
  • Author(s): Nagao, K, Kimoto, Y, Marumi, K, Tsujimura, A, Vail, G. M., Watts, S, Ishii, N, Kamidono, S
  • Journal Title: Urology 68 Pages: 845-851
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation of germ cells from leukemia and lymphoma cells in a human/ in vitro/ model : potential clinical application for restoring human fertility after anticancer therapy (2006)
  • Author(s): Fujita, K, Tsujimura, A, Miyagawa, Y, Kiuchi, H, Matsuoka, Y, Takao, T, Takada, S, Nonomura, N, Okuyama, A
  • Journal Title: Cancer Res 66 Pages: 11166-11671
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain processing of audiovisual sexual stimuli inducing penile erection : A positron emission tomography study (2006)
  • Author(s): Tsujimura, A, Miyagawa, Y, Fujita, K, Matsuoka, Y, Takahashi, T, Takao, T, Matsumiya, K, Osaki, Y, Takasawa, M, Oku, N, Hatazawa, J, Kaneko, S, Okuyama, A
  • Journal Title: J. Urol 176 Pages: 679-683
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rare case of aggressive angiomyxoma presenting as a retrovesical tumor (2006)
  • Author(s): Hatano, K., Tsujimoto, Y., Ichimaru, N., Miyagawa, Y., Nonomura, N., Okuyama, A
  • Journal Title: Int. J. Urol 13 Pages: 1012-4
  • NAID: 10018173616
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of Mina53, a product of a Myc target gene in mouse testis (2006)
  • Author(s): Tsuneoka, M, Nishimune, Y, Ohta, K, Teye, K, Tanaka, H, Soejima, M, Iida, H, Inokuchi, T, Kimura, H, Koda, Y
  • Journal Title: Int J Androl 29 Pages: 323-330
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mitochondria-related male infertility (2006)
  • Author(s): Nakada, K, Sato, A, Yoshida, K, Morita, T, Tanaka, H, Inoue, S, Yonekawa, H, Hayashi, J
  • Journal Title: Proc Natl Acad Sci U S A 103 Pages: 15148-15153
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis (2006)
  • Author(s): Fraisl, P, Tanaka, H, Forss-Petter, S, Lassmann, H, Nishimune, Y, Berger, J. A
  • Journal Title: Arch Biochem Biophys 451 Pages: 23-33
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Infertility Caused by Polymorphisms or Mutations in Spermatogenesis-Specific Genes (2006)
  • Author(s): Nishimune, Y, Tanaka, H
  • Journal Title: J Androl 27 Pages: 326-334
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mitochondria-related male infertility (2006)
  • Author(s): Nakada Kazuto 他 7名
  • Journal Title: Proc Natl Acad Sci USA 103 Pages: 15148-15153
• [Journal Article] A novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis. (2006)
  • Author(s): Fraisl Peter 他 5名
  • Journal Title: Arch Biochem Biophys 451 Pages: 23-33
• [Journal Article] Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein. (2006)
  • Author(s): Tanaka Hiromtsu 他 8名
  • Journal Title: International Journal of Andrology 29 Pages: 353-359
• [Journal Article] Expression of Mina53, a product of a Myc target gene in mouse testis. (2006)
  • Author(s): TsuneoKa M 他 9 名
  • Journal Title: International Journal of Andrology 29 Pages: 323-330
• [Journal Article] Infertility Caused by Polymorphisms or Mutations in Spermatogenesis-Specific Genes. (2006)
  • Author(s): Nishimune Yoshitake, Tanaka Hiromitsu
  • Journal Title: Journal of Andrology 27 Pages: 326-334
• [Journal Article] Molecular biological features of male germ cell differentiation. (2006)
  • Author(s): Tanaka Hiromitsu 他 7名
  • Journal Title: Reproductive Medicine and Biology 6 Pages: 1-9
  • NAID: 10018849469
• [Journal Article] 精子形成のメカニズム (2006)
  • Author(s): 田中宏光
  • Journal Title: 内分泌・糖尿病科 23 Pages: 142-154
• [Journal Article] Expression of Mina53, a product of a Myc target gene in mouse testis. (2006)
  • Author(s): Tsuneoka M 他9名
  • Journal Title: Int.J.Androl. (In Press)
• [Journal Article] Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein. (2006)
  • Author(s): Tanaka H 他8名
  • Journal Title: Int.J.Androl. (In Press)
• [Journal Article] Infertility caused by polymorphisms or mutations in spermatogenesis-specific genes. (2006)
  • Author(s): Nishimune Y, Tanaka H.
  • Journal Title: J.Androl. (In Press)
• [Journal Article] Vascular endothelial growth factor restores erectile function through an inhibition of apoptosis in diabetic rat penile crura (2005)
  • Author(s): Yamanaka, M, Shirai, M, Shiina, H, Tanaka, Y, Enokida, H, Tsujimura, A, Matsumiya, K, Okuyama, A, Dahiya, R
  • Journal Title: J. Urol 173 Pages: 318-323
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Outcome of gonadotropin therapy for male hypogonadotropic hypogonadism at University-affiliated male infertility centers : A 30-years retrospective study (2005)
  • Author(s): Miyagawa, Y, Tsujimura, A, Matsumiya, K, Takao, T, Tohda, A, Koga, M, Takeyama, M, Fujioka, H, Takada, S, Koide, T, Okuyama, A
  • Journal Title: J. Urol 173 Pages: 2072-2075
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of inhibin alpha, GDNF and SCF in Sertoli cell-only syndrome : relation to successful sperm retrieval by microdissection testicular sperm extraction (2005)
  • Author(s): Fujita, K, Tsujimura, A, Takao, T, Miyagawa, Y, Matsumiya, K, Koga, M, Takeyama, M, Fujioka, H, Aozasa, K, Okuyama, A
  • Journal Title: Hum. Reprod 20 Pages: 2289-2294
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation and transplantation of spermatogonial stem cells for treatment of azoospermia after chemotherapy in a leukemic mouse model (2005)
  • Author(s): Fujita, K, Ohta, H, Tsujimura, A, Takao, T, Miyagawa, Y, Takada, S, Matsumiya, K, Wakayama, T, Okuyama, A
  • Journal Title: Hum. Reprod 20 Pages: 2289-2294
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sperm flagella protein components : Human meichroacidin constructed by the MORN motif (2005)
  • Author(s): Matsuoka, Y, Nishimura, H, Numazawa, K, Tsuchida, J, Miyagawa, Y, Tsujimura, A, Matsumiya, K, Okuyama, A, Nishimune, Y, Tanaka, H
  • Journal Title: Reprod. Med. & Biol 4 Pages: 213-219
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single-nucleotide polymorphisms and mutation analyses of the TNP1 and TNP2 genes of fertile and infertile human male populations (2005)
  • Author(s): Miyagawa, Y, Nishimura, H, Tsujimura, A, Matsuoka, Y, Matsumiya, K, Okuyama, A, Nishimune, Y, Tanaka, H
  • Journal Title: J. Androl 26 Pages: 779-786
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Impact of age, FSH and Johnsen's score on successful sperm retrieval by microdissection testicular sperm extraction (2005)
  • Author(s): Tsujimura, A, Miyagawa, Y, Takao, T, Fujita, K, Komori, K, Matsuoka, Y, Takada, S, Koga, M, Takeyama, M, Fujioka, H, Matsumiya, K, Okuyama, A
  • Journal Title: Reprod. Med. & Biol 4 Pages: 53-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative study on evaluation methods for serum testosterone level for PADAM diagnosis (2005)
  • Author(s): Tsujimura, A, Matsumiya, K, Miyagawa, Y, Takao, T, Fujita, K, Takada, S, Koga, M, Iwasa, A, Takeyama, M, Okuyama, A
  • Journal Title: Int. J. Imp. Res 17 Pages: 259-263
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human chorionic gonadotropin treatment for PADAM : A preliminary report (2005)
  • Author(s): Tsujimura, A, Matsumiya, K, Takao, T, Miyagawa, Y, Takada, S, Koga, M, Iwasa, S, Takeyama, M, Okuyama, A
  • Journal Title: Aging Male 8 Pages: 175-179
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Retroperitoneoscopic living related-donor nephrectomy : clinical outcomes of 50 consecutive cases and comparison with open donor nephrectomy (2005)
  • Author(s): Yoshimura, K, Takahara, S, Kyakuno, M, Yamaguchi, S, Yoshioka, T, Miyake, O, Tsujihata, M, Ichimaru, N, Miyagawa, Y, Imamura, R, Okuyama, A
  • Journal Title: J. Endourol 19 Pages: 808-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Association study of homologous RNA-binding-motif-gene on X chromosome (RBMX) and like sequence on chromosome 9 (RBMXL9) with non-obstructive azoospermia. Asian J. Androl. 8(2) : 213-8 (2005)
  • Author(s): Tsujimura, A., Fujita, K., Komori, K., Miyagawa, Y., Takada, S., Matsumiya, K., Sada, M., Katsuyama, Y., Ota, M., Okuyama, A
  • Journal Title: Asian J. Androl 8(2) Pages: 213-218
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Salvage microdissection testicular sperm extraction after failed conventional TESE for patients with non-obstructive azoospermia (2005)
  • Author(s): Tsujimura, A., Miyagawa, Y., Takao, T., Takada, S., Koga, M., Takeyama, M., Matsumiya, K., Fujioka, H., Okuyama, A
  • Journal Title: J. Urol 175(4) Pages: 1446-1495
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tisp40, a spermatid specific bZip transcription factor, functions by binding to the unfolded protein response element via the Rip pathway (2005)
  • Author(s): Nagamori, I, Yabuta, N, Fujii, T, Tanaka, H, Yomogida, K, Nishimune, Y, Nojima, H
  • Journal Title: Genes Cells 10 Pages: 575-594
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of Human HAPRIN Potentially Involved in the Acrosome Reaction (2005)
  • Author(s): Kitamura, K, Nishimura, H, Nishimune, Y, Tanaka, H
  • Journal Title: J Androl 26 Pages: 511-518
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of the mammalian sperm (2005)
  • Author(s): Ihara, M, Kinoshita, A, Yamada, S, Tanaka, H, Tanigaki, A, Kitano, A, Goto, M, Okubo, K, Nishiyama, H, Ogawa, O, Takahashi, C, Itohara, S, Nishimune, Y, Noda, M, Kinoshita, M
  • Journal Title: Developmental Cell 8 Pages: 343-352
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain processing of audiovisual sexual stimuli inducing penile erection : A positron emission tomography study (2005)
  • Author(s): Tsujimura, A., Miyagawa, Y., Fujita, K., Matsuoka, Y., Takahashi, T., Takao, T., Matsumiya, K., Osaki, Y., Takasawa, M., Oku, N., Hatazawa, J., Kaneko, S., Okuyama, A
  • Journal Title: J. Urol 176 Pages: 679-83
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Significance of electro stimulation in detecting neurovascular bundle during radical prostatectomy (2005)
  • Author(s): Tsujimura, A., Miyagawa, Y., Takao, T., Matsumiya, K., Nakayama, M., Tsujimoto, Y., Takaha, N., Nishimura, K., Nonomura, N., Takada, T., Fujioka, H., Kurokawa, K., Aozasa, K., Okuyama, A
  • Journal Title: Int. J. Urol 13(7) Pages: 926-31
  • NAID: 10018173286
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The RING-fmger protein Haprin : domains and f niction in the acrosome reaction (2005)
  • Author(s): Kitamura, K, Tanaka, H, Nishimune, Y
  • Journal Title: CPPS : Current Protein and Peptide Science 6 Pages: 567-574
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene expression in spermiogenesis (2005)
  • Author(s): Tanaka, H, Baba, T
  • Journal Title: CMLS : Cellular and Molecular Life Sciences 62 Pages: 344-354
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa. (2005)
  • Author(s): Ihara M 他14名
  • Journal Title: Dev.Cell 8 Pages: 343-352
• [Journal Article] Tisp40, a spermatid specific bZip transcription factor, functions by binding to the unfolded protein response element via the Rip pathway. (2005)
  • Author(s): Nagamori I 他6名
  • Journal Title: Genes Cells 10 Pages: 575-594
• [Journal Article] cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis. (2005)
  • Author(s): Somboonthum P 他6名
  • Journal Title: Nucleic Acids Res. 33 Pages: 3401-3411
• [Journal Article] Identification of human HAPRIN potentially involved in the acrosome reaction. (2005)
  • Author(s): Kitamura K 他3名
  • Journal Title: J.Androl. 26 Pages: 511-518
• [Journal Article] HANP1/H1T2, a novel histone H1-like protein involved in nuclear formation and sperm fertility. (2005)
  • Author(s): Tanaka H 他11名
  • Journal Title: Mol.Cell.Biol. 25 Pages: 7107-7119
• [Journal Article] Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions. (2005)
  • Author(s): Masuyama S 他8名
  • Journal Title: Genes Cells 10 Pages: 753-762
• [Journal Article] Single-nucleotide polymorphisms and mutation analyses of the TNP1 and TNP2 genes of fertile and infertile human male populations. (2005)
  • Author(s): Miyagawa Y 他7名
  • Journal Title: J.Androl. 26 Pages: 779-786
• [Journal Article] The RING-finger protein haprin : domains and function in the acrosome reaction. (2005)
  • Author(s): Kitamura K 他2名
  • Journal Title: Curr.Protein Pept.Sci. 6 Pages: 567-574
• [Journal Article] Sperm flagella protein components : Human meichroacidin constructed by the MORN motif. (2005)
  • Author(s): Tanaka H 他9名
  • Journal Title: Reprod.Med.Biol. 4 Pages: 213-219
• [Journal Article] Relationship between the substances in seminal plasma and the Acrobeads test[○!R]
  • Author(s): Komori, K., Tsujimura, A., Hirai, T., Okamoto, Y., Matsuoka, Y., Takao, T., Miyagawa, Y., Takada, S., Okuyama, A
  • Journal Title: Fertil & Steril (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Androgen Decline in Patients with Nonobstructive Azoospemia After Microdissection Testicular Sperm Extraction
  • Author(s): Takada, S, Tsujimura, A, Ueda, T, Matsuoka, Y, Takao, T, Miyagawa, Y, Koga, M, Takeyama, M, Okamoto, Y, Matsumiya, K, Fujioka, H, Nonomura, N, Okuyama, A
  • Journal Title: Urology (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Meichroacidin containing the MORN motif is essential for spermatozoa morphogenesis
  • Author(s): Tokuhiro, K, Hirose, M, Miyagawa, Y, Tsujimura, A, Irie, S, Isotani, A,Okabe, M, Toyama, Y, Ito, C, Toshimori, K, Takeda, K, Oshio, S, Tainaka, H, Tsuchida, J, Okuyama, A, Nishimune, Y, Tanaka, H
  • Journal Title: J Biol Chem (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] マウス精子形成におけるmeichroacidin (MCA)の機能解析 (2007)
  • Author(s): 徳弘 圭造, 廣瀬 未果, 宮川 康, 辻村 晃, 磯谷 綾子, 岡部 勝, 外山 芳郎, 伊藤 千鶴, 年森 清隆, 武田 健, 押尾 茂, 田井中均, 土田 順次, 奥山 明彦, 西宗 義武, 田中 宏光
  • Organizer: 日本アンドロロジー学会第26回学術大会
  • Place of Presentation: 千葉県
  • Year and Date: 2007-07-06
• [Presentation] Progress of ED research and practice in Asia. (2007)
  • Author(s): Akira Tsujimura
  • Organizer: The 4th congress of The Asia Pacific Society for the Study of Aging Male
  • Place of Presentation: Indonesia
  • Year and Date: 2007-05-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Progress on invasive management for erectile dysfunction. Main symposium 1 "ED & sexual function. (2007)
  • Author(s): Akira Tsujimura
  • Organizer: The 4th congress of The Asia Pacific Society for the Study of Aging Male
  • Place of Presentation: Indonesia
  • Year and Date: 2007-05-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Causal relationship between obesity and dyslipidemia with ED "ED and LUTS/BPH: Possible Relationship with Metabolic Syndrome". (2007)
  • Author(s): Akira Tsujimura
  • Organizer: The 11th Biennial Meeting of the Asia Pacific Society for Sexual Medicine
  • Place of Presentation: Korea
  • Year and Date: 2007-05-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Progress of ED research and practice in Asia (2007)
  • Author(s): Tsujimura, Akira
  • Organizer: The 4th congress of The Asia Pacific Society for the Study of Aging Male
  • Place of Presentation: Indonesia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Progress on invasive management for erectile dysfunction. Main symposium 1"ED & sexual function". Akira Tsujimura (2007)
  • Author(s): Tsujimura, Akira
  • Organizer: The 4th congress of The The 4th congress of The Asia Pacific Society for the Study of Aging Male
  • Place of Presentation: Indonesia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Causal relationship between obesity and dyslipidemia with ED "ED and LUTS/BPH : Possible Relationship with Metabolic Syndrome" (2007)
  • Author(s): Akira, Tsujimura
  • Organizer: The 11th Biennial Meeting of the Asia Pacific Society for Sexual Medicine
  • Place of Presentation: Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 精子細胞特異的遺伝子群の解析とその臨床応用 (2007)
  • Author(s): 田中宏光 廣瀬 未果 徳弘 圭造, 田井中均 松岡庸洋 宮川 康, 辻村 晃 奥山 明彦, 西宗 義武,
  • Organizer: 大阪大学 イノベーションセンター 研究シーズ発表の場
  • Place of Presentation: 大阪府
• [Presentation] 精子細胞核Kinase Haspinのpromoterは排卵後の卵でタンパク質発現を誘導する (2007)
  • Author(s): 田中宏光 徳弘 圭造, 廣瀬未果, 宮川 康
  • Organizer: 文部科学省 科学研究補助金 特定領域研究「生殖細胞の発生プロセス・再プログラム化とエピジェネティクス」公開シンポジウム
  • Place of Presentation: 東京都
• [Presentation] Challenging trends in managing metabolic disorders in the Asian Pacific Region. (2006)
  • Author(s): Akira, Tsujimura, Akihikom Okuyama
  • Organizer: The 5th International Society for Study of Aging
  • Place of Presentation: Austria
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Salvage microdissection testicular sperm extraction after failed conventional TESE for patients with non-obstructive azoospermia (2006)
  • Author(s): Akira, Tsujimura, Yasushi, Miyagawa, Kazutoshi, Fujita, Tetsuya, Takao, Suita, Masami, Takeyama, Masaki, Yamanaka, Kiyomi, Matsumiya, Hideki, Fujioka, Minoru, Koga, Mino, Akihiko, Okuyama
  • Organizer: AUA 101th Annual Meeting
  • Place of Presentation: USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] THE CLINICAL SIGNIFICANCE OF POLYMORPHISMS IN THE PROTAMINE & TRANSITION NUCLEAR PROTAIN GENES IN MALE INFERTILITY (2006)
  • Author(s): Yasushi, Miyagawa, Hiromitsu, Tanaka, Yasuhiro, Matsuoka, Tomohiro, Ueda, Kiuchi, Hiroshi, Phanu, Tanjapatkul, Kazutoshi, Fujita, Kazuhiko, Komori, Tetsuya, Takao, Shingo, Takada, Akira, Tsujimura, Yoshitake, Nishimune, Akihiko, Okuyama
  • Organizer: AUA 101th Annual Meeting
  • Place of Presentation: USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Experience from Japan, Symposium "Men's Health in ASEAN-Japan" (2006)
  • Author(s): Akira, Tsujimura
  • Organizer: The 1st Japan-ASEAN Men's Health and Aging Conference
  • Place of Presentation: Malaysia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Single nucleotide polymorphism (SNP)s discovery of Genetic causes on male fertility (2006)
  • Author(s): Hiromitsu, Tanaka
  • Organizer: 10th International Symposium on Spermatology
  • Place of Presentation: Madrid
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Single-Nuceotide Polymorphisms and Mutation Analyses of haploid germ cell-specific genes (2006)
  • Author(s): Hiromitsu, Tanaka
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The role of cerebellar activation in the initiation of human penile erection (2005)
  • Author(s): Akira, Tsujimura, Kazutoshi, Fujita, Kazuhiko, Komori, Yasuhiro, Matsuoka, Tohru, Takahashi, Tetsuya, Takao, Shingo, Takada, Kiyomi, Matsumiya, Yasnhiro, Osaki, Naohiko, Oku, Jun, Hatazawa, Akihiko, Okuyama
  • Organizer: AUA 100th Annual Meeting
  • Place of Presentation: USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparative study between sperm motility analysis system and conventional microscopic semen analysis based on WHO laboratory manual (2005)
  • Author(s): Akira, Tsujimura, Kazuhiko, Komori, Kazutoshi, Fujita, Tetsuya, Takao, Yasushi, Miyagawa, Shingo, Takada, Kiyomi, Matsumiya, Akihiko, Okuyama
  • Organizer: The 8th Intemational Congress of Anthology
  • Place of Presentation: Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification and Characterization of genes encoding testis-specific proteins involved in making spermatozoa (2005)
  • Author(s): Yoshitake, Nishimune, Hiromitsu, Tanaka
  • Organizer: 8th International Congress ofAndrology
  • Place of Presentation: Korea
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ED with lifestyle disease of aging male in Japan (2005)
  • Author(s): Akira, Tsujimura, Akihiko, Okuyama
  • Organizer: The 10th Biennial meeting of the Asia Pacific Society for Sexual & Impotence Research
  • Place of Presentation: Australia
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Hanp 1/H1T2, a novel histone-like protein involved in the formation of nucleus and the fertility of sperm (2005)
  • Author(s): Hiromitsu, Tanaka
  • Organizer: International Symposium on Germ Cells, Epigenetics, Reprogramming and Embryonic Stem Cells
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 蛋白質核酸酵素 (2007)
  • Author(s): 田中宏光
  • Publisher: 共立出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Reprod Med Biol (2007)
  • Author(s): Tanaka H
  • Publisher: Blackwell Science Asia
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Soc Reprod Fertil Suppl. (2007)
  • Author(s): Tanaka H
  • Publisher: Nottingham University Press,
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Int. J. Urol. (2007)
  • Author(s): Tsujimura A
  • Publisher: Blackwell Science Asia
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 蛋白質核酸酵素 「生殖細胞の発生・エピジェネティクスと再プログラム化」 (2007)
  • Author(s): 田中宏光
  • Total Pages: 2006
  • Publisher: 共立出版
• [Book] 精子形成関連遺伝子とその特異的発現 (精子学) (2006)
  • Author(s): 田中宏光, 西宗義武
  • Total Pages: 14
  • Publisher: 東京大学出版会
• [Book] 精子学 第10章 精子形成関連遺伝子とその特異的発現 (2006)
  • Author(s): 田中宏光, 西宗義武
  • Publisher: 東京大学出版会(In Press)
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.geocities.jp/magical_yellow_boots/
• [Remarks]
  • URL: http://www.geocities.jp/magical_yellow_boots/
• [Patent(Industrial Property Rights)] 妊孕性診断キット、変異検出方法、ポリヌクレオチド、ポリペプチド、発現ベクター、及び発現キット (2007)
  • Inventor(s): 田中宏光, 西宗義武, 奥山明彦, 辻村晃, 宮川康
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 2007-082574
  • Filing Date: 2007-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 妊孕性診断キット、変異検出方法、ポリヌクレオチド、ポリペプチド、発現ベクター、及び発現キット (2007)
  • Inventor(s): 田中宏光, 西宗義武, 奥山明彦, 辻村晃, 宮川康, 入江新司
  • Industrial Property Rights Holder: 大阪大学凸版印刷
  • Industrial Property Number: 2007-184946
  • Filing Date: 2007-07-13
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] マウス精子形成遺伝子とヒト男性不妊関連遺伝子、ならびにこれらを用いた診断システム (2007)
  • Inventor(s): 西宗義武, 田中宏光, 野崎正美
  • Industrial Property Rights Holder: 科学技術振興事業団
  • Acquisition Date: 2007-03-30
  • Description: 「研究成果報告書概要(和文)」より
  • Overseas
• [Patent(Industrial Property Rights)] 疾患モデル動物、細胞、組織、精巣、交配用動物、生殖細胞生産方法、生殖細胞、培養細胞、スクリーニング方法、ポリヌクレオチド、ポリペプチド、及び抗体 (2007)
  • Inventor(s): 田中宏光、西宗義武、奥山明彦、辻村晃、宮川康、入江新司
  • Industrial Property Rights Holder: 大阪大学凸版印刷
  • Industrial Property Number: 2007-184946
  • Filing Date: 2007-07-13
• [Patent(Industrial Property Rights)] 妊よう性診断キット、変異検出法、ポリヌクレオチド、及びポリペプチド (2006)
  • Inventor(s): 田中宏光, 他4名
  • Industrial Property Rights Holder: 大阪大学
  • Filing Date: 2006-09-14
• [Patent(Industrial Property Rights)] 疾患モテル動物、細胞、組織、精巣、交配用動物、生殖細胞生産方法、生殖細胞、培養細胞、妊よう性診断キット、変異検出法 (2006)
  • Inventor(s): 田中宏光, 西宗義武
  • Industrial Property Rights Holder: 大阪大学
  • Filing Date: 2006-02-28
• [Patent(Industrial Property Rights)] 特許 (2005)
  • Inventor(s): 田中宏光, 西宗義武
  • Industrial Property Rights Holder: 大阪大学知的財産本部
  • Industrial Property Number: 2005-279598
  • Filing Date: 2005-09-27