| Project/Area Number |
17380108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Kyoto University |
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Principal Investigator |
HAYASHI Takahisa Kyoto University, RISH, Associate professor (70231529)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Keiichi Kyoto UNIV, RISH, Assistant (20238223)
TAKEDA Takumi Kyoto UNIV, RISH, Researcher (80423036)
YOSHIDA Masato Nagoya UNIV, Graduate School of Bioagricultural Sciences School Agricultural Sciences Biosphere Resources Science, Associate professor (30242845)
TANIGUCHI Toru 林木育種センター, 育種部育種工学課, 室長 (00360470)
KAIDA Rumi Japan Women's UNIV, Department of Science, Researcher (00398885)
金子 堯子 (金子 尭子) 日本女子大学, 理学部, 教授 (80060680)
近藤 禎二 京都大学, ・材木育種センター育種部育種工学課, 課長 (20370827)
望月 正雄 大阪大学, 大学院・生命機能研究所, 特任助手 (20379085)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2006: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2005: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | G-layer / xyloglucan / cellulose / xyloglucan endotransglycosylase / gravitropical response / tension / エンドトランスグルコシラーゼ / キシログルカン架橋 / G層 / pttXTH6 / pttXET16B |
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| Research Abstract |
We produced transgenic poplar plants overexpressing several endoglycanases to reduce each specific polysaccharide in the cell wall, as the secondary xylem consists of primary and secondary wall layers. When placed horizontally, the basal regions of stems of transgenic poplars overexpressing xyloglucanase alone could not bend upward due to low strain in the tension side of the xylem. In the wild-type plants, xyloglucan was found in the inner surface of G-layers during multiple layering. In situ xyloglucan endotransglucosylase (XET) activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, began at the inner surface layers S1 and S2 and was retained throughout G-layer development, while the incorporation of xyloglucan heptasaccharide (XXXG) for wall loosening occurred in the primary wall of the expanding zone. We propose that the xyloglucan network is reinforced by XET to form a further connection between wall-bound and secreted xyloglucans in order to withstand the tensile stress created within the cellulose G-layer microfibrils.
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