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Formation of isolated DNA bases pairs and their water clusters

Research Project

Project/Area Number 17550017
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionGraduate School, Yokohama City University

Principal Investigator

SAIGUSA Hiroyuki  Yokohama City University, Graduate School of Arts and Sciences, Professor, 国際総合科学研究科, 教授 (90162180)

Co-Investigator(Kenkyū-buntansha) TACHIKAWA Masanori  Yokonarna city Vnversity, Graduate School of Artsand Scoences, Associate Professor, 国際総合科学研究科, 教授 (00267410)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsLaser Desoption / DNA bases / Hydrated clusters / Nucleosides / Tautomerization
Research Abstract

We have developed a novel laser desorption method in combination with the supersonic-jet technique for producing cold isolated clusters of the DNA bases and their hydrated clusters. This method is found to be especially effective in producing hydrated clusters of the nonvolatile molecules and thus useful in the microscopic understanding of hydration structures of DNA. The results show that excited-state dynamics of the base pairs are strongly dependent on the tautomers of the respective bases. For example, the purine base guanine reveals a tautomers of the canonical form whose excited-state lifetime is on the order of subpicosecond while other tautomers of non-canonical forms have nanosecond lifetimes. Furthermore, excited-state dynamics of adenine are altered significantly upon hydration. This is explained by the ultrafast relaxation of the initially excited state to a low-lying excited state and subsequent dissociation. This is found to be consistent with the result for 2-aminopurine in which no ultrafast dynamics occurs and thus is known to be strongly fluorescent.
We have also succeeded in producing hydrated clusters of the DNA nucleoside guanosines with high efficiency by employing this desorption technique. This is the first report of the observation of cold hydrated clusters of the nucleosides under isolated conditions. The electronic spectra of guanosines are found to be significantly red-shifted upon hydration, suggesting that the excited state-dynamics are affected by the solvation.
This laser desorption technique is expected to be effective for vaporizing thermally fragile biomolecules

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2007 2006 2005

All Journal Article (17 results)

  • [Journal Article] Tautomerization behavior in guanine and methylated guanines, and its relation to the base pairing and hydration2007

    • Author(s)
      K.Miyazaki, N.Mizuno, T.Katayama, K.Takahashi, M.Tachikawa, H.Saigusa
    • Journal Title

      Physical Chemistry and Chemical Physics (submitted)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Excited-State Dynamics of Nucleic Acid Bases and Their Clusters2006

    • Author(s)
      H.Saigusa
    • Journal Title

      Journal of Photochemistry and Photobiology C : Photochemistry Reviews 7

      Pages: 197-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      H.Saigusa, A.Tomioka, T.Katayama, E.Iwase
    • Journal Title

      Chemical Physics Letters 418・1

      Pages: 119-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Excited-state dynamics of nucleic acid base and their clusters2006

    • Author(s)
      H.Saigusa
    • Journal Title

      Journal of Photochemistry and Photobiology C : Photochemistry Reviews 7

      Pages: 197-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      H.Saigusa, A.Tomioka, T.Katayama, E.Iwase
    • Journal Title

      Chemical Physics Letters 418(1)

      Pages: 119-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Excited-State Dynamics of Nucleic Acid Bases and Their Clusters2006

    • Author(s)
      Hiroyuki Saigusa
    • Journal Title

      journal of Photochemistry and Photobiology C : Photochemistry Reviews 7

      Pages: 197-210

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      Hiroyuki Saigusa, Azusa Tomioka, Takumi katayama, Eijiro Iwase
    • Journal Title

      Chemical Physics Letters 418・1

      Pages: 119-125

    • Related Report
      2006 Annual Research Report
  • [Journal Article] レーザー脱離法を用いたDNA塩基分子の気相分光2006

    • Author(s)
      三枝洋之
    • Journal Title

      日本化学会東北支部郡山地区講演会招待講演(11月17日、日本大学工学部)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] グアニンにおける水和と塩基対生成との関連 : メチル変換による検証2006

    • Author(s)
      宮崎 謙二, 片山 巧, 三枝 洋之
    • Journal Title

      分子構造総合討論会2006要旨集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] グラファイトマトリクスを用いたアデニン類のレーザー脱離2006

    • Author(s)
      三枝洋之, 水野信輝, 宮崎謙二
    • Journal Title

      分子構造総合討論会2006要旨集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 気相中のグアニン異性体のダイマー形成に関する理論的分析2006

    • Author(s)
      高橋研志, 三枝洋之, 立川仁典
    • Journal Title

      分子構造総合討論会2006要旨集

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pairing and Hydration of Guanine Tautomers2006

    • Author(s)
      H.SAIGUSA
    • Journal Title

      Sendai International Symposium on Molecular Cluster Spectroscopy, Sendai (poster, May 29, 2006)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Excited-state dynamics of hydrated clusters of the nucleobases2006

    • Author(s)
      H.SAIGUSA
    • Journal Title

      XXIst IUPAC Symposium on Photochemistry, Kyoto (poster, Apr.4, 2006)

      Pages: 52-52

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      Hiroyuki Saigusa, Azusa Tomioka, Takumi Katayama, Eijiro Iwase
    • Journal Title

      Chemical Physics Letters 418・1

      Pages: 119-125

    • Related Report
      2005 Annual Research Report
  • [Journal Article] DNA塩基水和クラスターの構造と励起ダイナミクス2005

    • Author(s)
      三枝洋之, 片山匠, 林晃夫, 徳村邦弘
    • Journal Title

      分子構造総合討論会2005要旨集

    • Related Report
      2005 Annual Research Report
  • [Journal Article] レーザー脱離法によるグアニン-シトシン塩基クラスターとその水和物の生成2005

    • Author(s)
      片山匠, 三枝洋之
    • Journal Title

      分子構造総合討論会2005要旨集

    • Related Report
      2005 Annual Research Report
  • [Journal Article] DNA塩基の微細水和構造とダイナミクス2005

    • Author(s)
      三枝洋之
    • Journal Title

      分子研研究会「多様な水素結合系と量子効果」要旨集

      Pages: 13-13

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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