• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Screening of new abused drugs by Solid-Phase Microextraction

Research Project

Project/Area Number 17590587
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionFujita Health University

Principal Investigator

HAMAJIMA Makoto  Fujita Health University, Department of Legal Medicine, School of Medicine, assistant professor (20189608)

Co-Investigator(Kenkyū-buntansha) KANEKO Rina  Fujita Health University, Department of Legal Medicine, School of Medicine, assistant professor (70367697)
HIRATA Yukari  Fujita Health University, Department of Legal Medicine, School of Medicine, assistant professor (50156676)
ISHII Akira  Nagoya University, Department of Legal Medicine and Bioethics, Graduate School of Medicine, professor (30252175)
勝又 義直  名古屋大学, 大学院・医学系研究科, 教授 (30109326)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,690,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥90,000)
Fiscal Year 2007: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2006: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsSolid-phase microextraction(SPME) / Organoahosphate / Flame photometric detector(FPD) / Triage DOA / Liquid-liquid extraction / Abused drugs / スクリーニング
Research Abstract

In this report, we present a Solid-phase microextraction (SPME) method for determination of Organophosphate pesticides in human whole blood. The whole blood samples containing eight petsticides (dichlorvos, fenitnothion, malathion, fenthion, profenofos, isoxathion, ethion and phosalone) were heated at 50℃ in a septum-cappedvial in the presence of K_2CO_3, and an SPME fiber was exposed in the vial for 30 min. Immediately after withdrawal of the fiber, it was analyzed by gas chromatography with FPD (GC/FPD). Recoveries of the eight pesticides were 0.03-2.19%. The calibration curves for the pesticides showed linearity in the range of 25-400ng/0.5ml whole blood except for phosalone (200-800ng/0.5ml whole blood) with detection limits of 0.2-25ng/0.5ml whole blood except for phosalone (20ng/ 0.5ml whole blood).
In conclusion, we have been able to extract and detect organophosphate pesticides in human whole bloods by use of the headspace SPME - GC/FPD. From the view points of simplicity, background noises, detection limit and quantitative, the present method can be recommended before analyses by GC in forenc, clinical, veterinary and environmental toxicology.
We have devised a liquid-liquid extraction method for Triage DOA screening of the abused drugs in whole bloods. The drugs were extracted from the matrices by fluid-fluid extraction using diethyl ether. The dection limits were 10ng/ml(Phencyclidine), 90ng/ml (Alprazolam), 140ng/ml (Diazepam), 300ng/ml (Nitrazepam), 80ng/ml (triazolam), 520ng/ml (Amphetamine), 400ng/ml (Methamphetaniine), 300ng/ml (Morphine), 120ng/ml (Codeine), 60ng/ml (Pentobarbital), 100ng/ml (Barbital), 160ng/ml (Phenobarbital), 200ng/ml (Amitriptyline), 200ng/ml (Imipramine), 400ng/ml (Nortriptyline).
In conclusion, our method is recommendable for the detection in forensic toxicology, because of its precision and sufficient sensitivity.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (42 results)

All 2007 2006 2005

All Journal Article (14 results) (of which Peer Reviewed: 3 results) Presentation (23 results) Book (5 results)

  • [Journal Article] Rapid analysis of seetraline, fluvoxamine, and paroxetine in serum specimens by LC-MS-MS using a new polymer column.2007

    • Author(s)
      Kumazawa, T., Ito, K., Iwai, M., Arinobu, T., Sato, K., et. al.
    • Journal Title

      Forensic Toxicol 25

      Pages: 100-103

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 法中毒学における新しい分析技術2006

    • Author(s)
      石井 晃
    • Journal Title

      藤田学園医学会誌 29

      Pages: 91-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sensitive analysis of aconitine,hypaconitine,mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS2006

    • Author(s)
      Kaneko, R.
    • Journal Title

      J Mass Spectrom. 41

      Pages: 810-814

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sensitive analysis of aconitine, hypaconitine, mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS.2006

    • Author(s)
      Kaneko, R.
    • Journal Title

      J. Mass Spectrom. 41

      Pages: 810-814

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sensitive analysis of aconitine, hypaconitine, mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS.2006

    • Author(s)
      金子理奈
    • Journal Title

      JOURNAL OF MASS SPECTROMETRY 41

      Pages: 810-814

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Seneitive analysis of aconitine, hypaconitine, and jesaconitine in human body fluids and Aconitum tubers by LC/ESI-TOF-MS2006

    • Author(s)
      Kaneko, R., hattori, S., Furuta, S., et al.
    • Journal Title

      J.Mass Spectrom. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] TOF-MSの法中毒への応用2005

    • Author(s)
      石井 晃
    • Journal Title

      法中毒 23

      Pages: 192-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sensitive determination of acetonitrile and propionitrile in human whole blood by headspace capillary gas chromatography with cryogenic oven trapping2005

    • Author(s)
      Kaneko, R
    • Journal Title

      Jpn.J.Forensic Toxicol. 23

      Pages: 37-40

    • NAID

      120005314067

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Application of TOF-MS to forensic toxicology2005

    • Author(s)
      Ishii, A.
    • Journal Title

      Jpn. J. Forensic Toxicol 23

      Pages: 192-197

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sensitive determination of acetonitril and propionitrile in human whole blood by headspace capillary gas chromatography with cryogenic oven trapping.2005

    • Author(s)
      Kaneko, R.
    • Journal Title

      Jpn. J. Forensic Toxicol 23

      Pages: 37-40

    • NAID

      120005314067

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] New analytical Techniques in forensic toxicology.2005

    • Author(s)
      Ishii, A.
    • Journal Title

      Bulletin. Fujit&Med. Soc. 29, (2)

      Pages: 91-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Problems in blood alcohol testing of severely injured drivers brought to emergency departments in Japan.2005

    • Author(s)
      Higuchi, A., Kurihara, R., Yoshimoto, T., Sano, T., Katsumata, K., Ishii, A., Katsumata, Y.
    • Journal Title

      Legal Medicine 7

      Pages: 299-305

    • NAID

      10027261637

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simultaneous analysis of nitrite and nitrate in whole blood by ion chromatography.2005

    • Author(s)
      Suzuki, O., Watanabe, K., Okamoto, N., Nozawa, H., Ishii, A.
    • Journal Title

      J.Liquid Chromatogr.Related Technolgies 28

      Pages: 3077-3085

    • Related Report
      2005 Annual Research Report
  • [Journal Article] TOF-MSの法中毒学への応用.2005

    • Author(s)
      石井 晃, 金子理奈, 平田ゆかり, 浜島 誠
    • Journal Title

      法中毒 23

      Pages: 192-197

    • Related Report
      2005 Annual Research Report
  • [Presentation] 背部切開が有用であった非交通外傷例について2007

    • Author(s)
      石井 晃
    • Organizer
      第29回日本法医学会中部地方会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-10-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 背部切開が有効であった非交通外傷例について2007

    • Author(s)
      石井 晃、浜島 誠、平田ゆかり
    • Organizer
      第29回日本法医学会中部地方会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-10-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] 低温オーブントラッピングGC/MSを用いた揮発性有機化合物の高感度一斉分析2007

    • Author(s)
      金子 理奈
    • Organizer
      第32回日本医用マススペクトル学会年会
    • Place of Presentation
      京都
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] レーザースプレーイオン化法によるヒト体液中向精神薬の高感度分析2007

    • Author(s)
      石井 晃
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 第二世代アレルギー薬のLC/MS/MSによる血中からの検出2007

    • Author(s)
      服部秀樹、岩井雅枝、他5名
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田
    • Year and Date
      2007-05-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Sensitive analysis of amanitins by LC-Biological fluids by liquid chromatography/electrospray-ion trap mass spectrometry.2007

    • Author(s)
      Ishii, A
    • Organizer
      The 91st Congress of the Japanese Society of Legal Medicine
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Highly sensitive and systematic asnalysis of volatile organic compounds using cryogenic oven trapping with GC/MS2007

    • Author(s)
      Kaneko, R.
    • Organizer
      The 30th Annual Meeting of Japanese Society for Biomedical Mass Spectrometry
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] LC/MSを用いた向精神薬の一斉分析2006

    • Author(s)
      金子 理奈
    • Organizer
      第28回日本法医学会中部地方会
    • Place of Presentation
      津
    • Year and Date
      2006-10-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] トライエージDOA[O!R],を用いた液-液抽出によるヒト全血中乱用薬物の簡易スクリーニング法の検討2006

    • Author(s)
      浜島 誠
    • Organizer
      日本法中毒学会第25年会
    • Place of Presentation
      東京
    • Year and Date
      2006-06-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] LC/MSによるヒト体液中アマニチン類の定量2006

    • Author(s)
      棚橋 昌一
    • Organizer
      第90次日本法医学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2006-04-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Simple screening for drugs of abuse in human whole blood using Triage DOA$ and liquid-liquid extraction.2006

    • Author(s)
      Hamajima, M.
    • Organizer
      The 25th Annual Meeting of Japanese Association of Forensic Toxiocology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 固相抽出を用いたヒト全血からのトライエージDOAによる薬物スクリーニング2005

    • Author(s)
      岩井 雅枝
    • Organizer
      第27回日本法医学会中部地方会
    • Place of Presentation
      名古屋
    • Year and Date
      2005-10-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] トライエージDOA,を用いた液-液抽出によるヒト全血中乱用薬物の簡易スクリーニング法の検討2005

    • Author(s)
      浜島 誠
    • Organizer
      第27回日本法医学会中部地方会
    • Place of Presentation
      名古屋
    • Year and Date
      2005-10-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 液体クロマトグラフィー/タンデム質量分析によるヒト体液中アマニチン類の高感度分析2005

    • Author(s)
      棚橋 昌一
    • Organizer
      第30回日本医用マススペクトル学会年会
    • Place of Presentation
      千里
    • Year and Date
      2005-09-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] SPME法によるピペラジン系乱用薬物の迅速抽出2005

    • Author(s)
      浜島 誠
    • Organizer
      第89次日本法医学会総会
    • Place of Presentation
      高松
    • Year and Date
      2005-04-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sensitive analysis of amanitins by LC-electrospray and laserspray ionization-MS2005

    • Author(s)
      Ishi A.
    • Organizer
      The 43rd Meeting of the International Association of Forensic Toxicologists
    • Place of Presentation
      ソウル
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sensitive determination of cyanid in human body fluids by cryogenic oven trapping(COT)-MS2005

    • Author(s)
      Kaneko R.
    • Organizer
      The 43rd Meeting of the International Association of Forensic Toxicologists
    • Place of Presentation
      ソウル
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 法医学研究における質量分析の応用2005

    • Author(s)
      石井 晃
    • Organizer
      第53回質量分析総合討論会
    • Place of Presentation
      さいたま
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sensitive asnalysis of amanitins by LC-electrospray and laserspray ionization-MS.2005

    • Author(s)
      Ishii, A.
    • Organizer
      The 43rd meeting of the International Association of Forensic Toxiocologists
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Application of Mass Spectrometry to Forensic Toxicology.2005

    • Author(s)
      Ishii, A.
    • Organizer
      The 53rd Annual Conference on Mass Spectrometry
    • Place of Presentation
      Saitama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Simple Analysis of Piperazine-like Abused Drugs by Microextraction(SPME) and GC.2005

    • Author(s)
      Hamajima, M.
    • Organizer
      The Japanese Jornal of Legal Medicine
    • Place of Presentation
      Takamatsu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sensitive analysis of amanitins in human Biological fluids by liquid chromatography/electrospray-ion trap mass spectrometry.2005

    • Author(s)
      Tanahashi, M.
    • Organizer
      The 30th Annual Meeting of Japanese Society for Biomedical Mass Spectrometry
    • Place of Presentation
      Senri
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Rapid and simple determination of selective serotonin reuptake inhibitor(SSRI) in human serum or plasma by LC/MS and LC/MS.2005

    • Author(s)
      Hattori, H.
    • Organizer
      The 24th Annual Meeting of Japanese Association of Forensic Toxiocology
    • Place of Presentation
      Fukuoka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Elsevier Science Ltd.Encyclopedia of Mass Spectrometry, Vol.82006

    • Author(s)
      Ishii, A.
    • Total Pages
      1067
    • Publisher
      Wilfred Niessen
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Surface ionization and its application to GC-MS. In ; Encyclopedia of Mass Spcetrometry Vo.82006

    • Author(s)
      Ishii, A., Suzuki, O., Katsumata, Y.
    • Publisher
      Elsevier Science Ltd.(In press)
    • Related Report
      2005 Annual Research Report
  • [Book] II. 2.8. Phencyclidine. In : Drugs and Poisons in Humans. A Handbook of Practical Analysis.2005

    • Author(s)
      Ishii, A, Katsumata, Y.
    • Publisher
      Springer-Verlag.
    • Related Report
      2005 Annual Research Report
  • [Book] II. 3.1. Phenothiazines. In : Drugs and Poisons in Humans. A Handbook of Practical Analysis.2005

    • Author(s)
      Ishii, A, Katsumata, Y.
    • Publisher
      Springer-Verlag.
    • Related Report
      2005 Annual Research Report
  • [Book] II. 7.7. Diazine and triazine herbicidese. In : Drugs and Poisons in Humans. A Handbook of Practical Analysis.2005

    • Author(s)
      Ishii, A, Katsumata, Y.
    • Publisher
      Springer-Verlag.
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi