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Proteomic analysis of Crow-Fukase syndrome

Research Project

Project/Area Number 17590888
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionKagoshima University

Principal Investigator

HASHIGUCHI Teruto  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (70250917)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Ikuro  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (20082282)
ABEYAMA Kazuhiro  Kagoshima University, Graduate School of Medical and Dental Sciences, Visiting Associate Professor (30284897)
ARIMURA Kimiyishi  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (20159510)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCrow-Fukase syndrome / POEMS syndrome / proteome / proteomics / peptidome / VEGF / brain / nerve / Syndrome
Research Abstract

Objective : This study aimed to establish a diagnostic method for detecting what molecules have been degraded and what is going to progress next, by comprehensively and rapidly identifying peptide fragments in the patient's serum throughout subsequent pathological stages, thereby developing a diagnostic method that contributes to determining the appropriate direction of strategies for assessment of the pathological condition, nutritional support, and drug treatment. Methods : New proteome analysis technology, developed by Protosera Inc., was applied for the differential profiling of untreated serum samples from normal subjects and the same patient with Crow-Fukase Syndrome (POEMS Syndrome),an intractable inflammatory disorder, in the stable (at the time of diagnosis),exacerbation, DIC, and MOF stages, and MS/MS de novo sequencing of significantly different MS peaks was performed. A similar analysis was conducted of untreated serum samples from normal subjects and patients with six different types of early cancer. Results : We identified 94 low-molecular-weight peptide fragments that characteristically appeared in the DIC and MOF stages. These peptide fragments were derived from carrier proteins, such as transthyretin and protease inhibitors such as α1-antitrypsin. Moreover, some of these 94 peptide fragments coincided with the peptide fragment peaks that were characteristically observed in the respective early cancer patients. Discussion : The patterns of low-molecular-weight peptide fragments that appear in the blood reflect the activation of proteases characterizing the biological environment, including that of normal subjects and patients with cancer, DIC, or MOF, thereby providing valuable information on pathological conditions. We consider that these results will lead to the development of a method that contributes to determining the appropriate direction of strategies for pathological condition diagnosis, nutritional support, and drug treatment.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (21 results)

All 2007 2006 2005 Other

All Journal Article (15 results) Book (2 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Serum VEGF-As a prognostic factor of atherosclerosis2007

    • Author(s)
      Kimura K, Hashiguchi T, et. al.
    • Journal Title

      Atherosclerosis 194(1)

      Pages: 182-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serum VEGF-As a prognostic factor of atherosclerosis2007

    • Author(s)
      Kimura, K, et. al.
    • Journal Title

      Atherosclerosis 194, (1)

      Pages: 182-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition2006

    • Author(s)
      Nakalima Y, Hashiguchi T, Maruvama I, et. al.
    • Journal Title

      FEBS Letters 580(2)

      Pages: 613-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cepharanthine triggers apoptosis in a human hepatocellular carcinoma cell line(HuH-7)through the activation of JNK1/2 and the downxegulation of Akt2006

    • Author(s)
      BisNas KK, Hashiguchi T, Maruyama I, et. al.
    • Journal Title

      FEBS Letters 580(2)

      Pages: 703-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition2006

    • Author(s)
      Nakajima, Y, et. al.
    • Journal Title

      FEBS Letters 580, (2)

      Pages: 613-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cepharanthine triggers apoptosis in, a human hepatocellular carcinoma cell line (HuH-7) through the activation of JNK1/2 and the downreaulation of Akt2006

    • Author(s)
      Biswas, KK, et. al.
    • Journal Title

      FEBS Letters 580, (2)

      Pages: 703-10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition.2006

    • Author(s)
      Nakajima Y, Hashiguchi T, Maruyama I, et al.
    • Journal Title

      FEBS Letters 580(2)

      Pages: 613-619

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cepharanthine triggers apoptosis in a human hepatocellular carcinoma cell line (HuH-7) through the activation of JNK1/2 and the downregulation of Akt.2006

    • Author(s)
      Biswas KK, Hashiguchi T, Maruyama I, et al.
    • Journal Title

      FEBS Letters 580(2)

      Pages: 703-710

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Activated protein C, a natural anticoagulant protein, has antioxidant properties and inhibits lipid peroxidation and advanced glycation end products formation2005

    • Author(s)
      Yamaji K, Hashiguchi T, Maruyama I, et. al.
    • Journal Title

      Thrombosis Research 115(4)

      Pages: 319-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma (NB-1)cells by argatroban2005

    • Author(s)
      Sarker KP, Hashiguchi T, Maruyama I, et. al.
    • Journal Title

      Pathophysiology of Haemostasis and Thrombosis 34(1)

      Pages: 41-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Activated protein C, a natural anticoagulant protein, has antioxidant properties and inhibits lipid peroxidation and advanced glycation end products formation2005

    • Author(s)
      Yamaji, K, et. al.
    • Journal Title

      Thrombosis Reserch 115, (4)

      Pages: 319-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma (NB-1) cells by argatroban2005

    • Author(s)
      Sarker, KP, et. al.
    • Journal Title

      Pathophysiology of Haemostasis and Thrombosis 34, (1)

      Pages: 41-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Activated protein C, a natural anticoagulant protein, has antioxidant properties and inhibits lipid peroxidation and advanced glycation end products formation.2005

    • Author(s)
      Yamaji K, Hashiguchi T, Maruyama I, et al.
    • Journal Title

      Thrombosis Research 115(4)

      Pages: 319-325

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma(NB-1) cells by argatroban.2005

    • Author(s)
      Sarker KP, Hashiguchi T, Maruyama I, et al.
    • Journal Title

      Pathophysiology of Haemostasis and Thrombosis 34(1)

      Pages: 41-47

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Serum VEGF-As a prognostic factor of atherosclerosis.

    • Author(s)
      Kimura K, Hashiguchi T, et. al.
    • Journal Title

      Atherosclerosis (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Book] Crow-Fukase syndrome:Clinical Featores, Pathogenesis and Treatment in Japan2006

    • Author(s)
      Arimura K and Hashiguchi T
    • Total Pages
      5
    • Publisher
      MEDIMOND S.r.l.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Crow-Fukase syndrome: Clinical Features, Pathogenesis and Treatment in Japan2006

    • Author(s)
      Arimura K and Hashiguchi T
    • Total Pages
      5
    • Publisher
      MEDIMOND S.r.l.
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規病態マーカー及びそれを用いた病態診断2006

    • Inventor(s)
      橋口 照人, 他3名
    • Industrial Property Rights Holder
      橋口 照人, 他3名
    • Industrial Property Number
      2006-169359
    • Filing Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 新規疾患マーカー及びそれを用いた診断2006

    • Inventor(s)
      橋口 照人, 他4名
    • Industrial Property Rights Holder
      橋口 照人, 他4名
    • Industrial Property Number
      2006-335121
    • Filing Date
      2006-12-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 新規病態マーカー及びそれを用いた病態診断2006

    • Inventor(s)
      橋口照人, 他3名
    • Industrial Property Rights Holder
      橋口照人, 他3名
    • Industrial Property Number
      2006-169359
    • Filing Date
      2006-12-12
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規疾患マーカー及びれを用いた診断2006

    • Inventor(s)
      橋口照人, 他4名
    • Industrial Property Rights Holder
      橋口照人, 他4名
    • Industrial Property Number
      2006-335121
    • Filing Date
      2006-06-19
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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