| Project/Area Number |
17591440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
ANDO Nobutoshi Tokyo Dental College, Dental, Professor (90101972)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Michio Tokyo Dental College, Dental, Assistant Professor (50215852)
OGAWA Shinji Tokyo Dental College, Dental, Assistant Professor (80224103)
HARADA Hiohisa Tokyo Dental College, Dental, Assistant Professor (70265841)
AOKI Seishi Tokyo Dental College, Dental, Assistant Professor (50296589)
正村 滋 東京歯科大学, 歯学部, 助教授 (40190342)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | implantation / regenerative medicine / cell culture / biotechnology |
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| Research Abstract |
1.Isolalon and culture of rat's esophageal keratinocytes
1) Donor rats were sacrifioed and pieces of esophageal mucosa were obtained. They were incubated in dispase solution and then stirred in trypsin solution. Rat's esophageal keratinocytes were successfully harvested.
2) Esophageal keratiocyies were successfully cultured by 3T3 feeder method on collagen gel.
2.Implantation of the esophageal keratinocytes sheet to the omentun or to the latissimus dorsi muscle and regeneration of neoesophagus
1) The collagen sheets with cuttured keratinocytes were implanted in recipient rat's latissimus dorsi muscle or in omentun and rolled and sutured. One 2, 3, week after implantation the tissue were taken and examined. They formed tube and neoesophagi were confirmed to regenerate macroscopically and microscopically.
3. Anastomosis between Neoesophagus and small intestine and replacement of the cervical esophagus with neoesophagus
1)Neoesophagus male in omentum were anastomosed to native jejunum in an end
… More
to end fashion. These rats also received Roux-Y operation and anastomoses were kept from food passage. Operated rats were all survived and the anastomoses were successfully made. Mucosa of neoesophagi were maintained and microscopically grew and became simliar to normal esophageal mucosa.
2) Neoesophagus made in latissimus dorsi muscle was pulled up to neck at the time of implantation. One week after implantation the 1cm cervical esophagus of recipient rat were resected and replaced with neoesophagus and anastomosed them an end to end fashion. The rats survived 6 days after the operation and anastomoses were successfully made.
4. Human esophageal keratinocyte culture and implantation to nude mice
1) Normal human esophageal pieces of mucosa were obtained from esophageal cancer patients and keratinocytes were harvested as above method. They were cultured on collagen gel with serum free medium.
2) The collagen sheets were implanted on latissimus dorsi muscle of nude mice. By 16 days the epithetial cel layer was similar to normal human esophageal epithetum. Less
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