Intrinsic stem cell promote neuronal regeneration by oligomerization of A β 1-42monomer
| Project/Area Number |
17591513
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
KONDOH Takeshi Kobe University, School of Medicine, Associate Professor (50273769)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTOQ Akira Foundation for Biomedical Research and Innovation, 脳疾患病態解析部, Director (80181759)
KOHMURA Eiji Kobe University, Graduate School of Medicire, Professor (30225388)
三宅 茂 神戸大学, 医学部附属病院, 助手 (30362784)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
|
|---|
| Keywords | Bone marrow stromal cells / organotopic hipocampal slice / 海馬切片培養 / 幹細胞 / ベータアミロイド / 骨髄間質細胞 |
|---|
| Research Abstract |
Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers of Sca-1 and Thy-1. The cells were then co-cultured on organotopic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.
|
Report
(4 results)
Research Products
(8 results)
-
-
-
-
-
-
-
[Journal Article] Impaired response of perforating arteries to hypercapnia in chronic hyperglycemia2006
Author(s)
Oizumi XS, Akisaki T, Kouta Y, Song XZ, Takata T, Kondoh T, Umetani K, Hirano M, Yatnasaki K, Kohmura E, Yokono K, Sakurai T.
-
Journal Title
Kobe J Med Sci. 52(2)
Pages: 27-35
Related Report
-
