Transplantation of GDNF-producing adult neural stem cells for cervical spondylosis model
| Project/Area Number |
17591514
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Okayama University |
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Principal Investigator |
MIYOSHI Yasuyuki Okayama University, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, SENIOR ASSISTANT PROFESSOR (00362997)
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| Co-Investigator(Kenkyū-buntansha) |
DATE Isao OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, PROFESSOR (70236785)
SUGIU Kenji OKAYAMA UNIVERSITY, HOSPITALS, SENIOR ASSISTANT PROFESSOR (40325105)
TOKUNAGA Kouji OKAYAMA UNIVERSITY, HOSPITALS, SENIOR ASSISTANT PROFESSOR (40294467)
ONO Shigeki OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, ASSISTANT PROFESSOR (40335625)
ICHIKAWA Tomotsugu OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, ASSISTANT PROFESSOR (10362964)
小野田 惠介 岡山大学, 大学院・医歯薬学総合研究科, 助手 (20379837)
松井 利浩 岡山大学, 大学院医歯薬学総合研究科, 助手 (80362995)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | GDNF / adult / neural stem cell / dial cells / Neuron / inflammatory cells / agarose / BBB scores / グリア / 頚髄症 / アドノウイルス / グリオーシス |
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| Research Abstract |
The appropriate model of cervical spondylosis has not been established, although the number of patients with cervical spondylosis was increasing. We attempted to make the model to put agarose beads extradurally. On making cervical spondylosis model of rat, slipped agarose beads might be the major problem. In order to oppress surely spine for a long lime, slipped agarose beads were prevented by Gore Tex sheet and wide laminotomy were also performed for the lateral compression. Fibrin glue was applied to the agarose beads placed epidurally with firm attachment of Gore Tex sheet. After using this method, no slipped agarose beads were recognized at euthanasia. In this cervical spondylosis model of rats, BBB scores were significantly deteriorated and histologically spinal damages were larger than that of the control rats. By the transplantation of GDNF-secreling neural stem cells, BBB scores were ameliorated, although the improvement was not significant Transplanted GDNF-secreting cells survived for at least 1 month and secreted GDNF. GDNF-concentration near the transplanted site was amplified about 5 times, compared to the normal tissue. Histologically, transplantation could not reduce damaged area significantly and inflammatory cells increased around the transplanted site. Prevention of enhanced inflammatory cells might be a key to improve the cell therapy far cervical spondylosis model of rats.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Comparison of the therapeutic potential of adult and embryonic neural precursor cells in a rat model of Parkinson disease2008
Author(s)
Muraoka, K., Shingo, T., Yasuhara, T., Kameda, M., Yuen, WJ., Uozumi, T., Matsui, T., Miyoshi, Y., Date, I
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Journal Title
Journal of Neurosurgery 108(1)
Pages: 149-159
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Adult neural stem and progenitor cells modified to secrete GDNF can protect, migrate and integrate aftex intracerebral transplantation in rats with transient forebrain ischemia2007
Author(s)
Kameda, M., Shingo, T., Takahashi, K., Muraoka, K., Kurozumi, K., Yasuhara, T., Maruo, T., Tsuboi, T., Uozumi, T., Matsui, T., Miyoshi, Y., Hamada, H., Date, I
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Journal Title
European Journal of Neuroscience 26
Pages: 1462-1478
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Encapsulated BDNF-producing cell graft has a neuroprotective effect and enhances neurogenesis in the damaged hippocampus2007
Author(s)
Shingo, T., Kuramoto, S., Yuan, W., Kondo, A., Kadota, T., Tajiri, N., Morimoto, T., Yoshida, H., Miyoshi, Y., Date, I
Organizer
Brain '07 & Brain PET '07 (The 23rd International Symposium on Cerebral Blood Flow, Metabolism & Function and The 8th International Conference on Quantification of Brain Function with PET)
Place of Presentation
Osaka
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Suppression of apoptosis by electrical stimulation on cerebral infarct model of rats2007
Author(s)
Baba, T., Shingo, T., Kameda, M., Morimoto, T., Tajiri, N., Miyoshi, Y., Yasuhara, T., Matsumae, M., Date, I
Organizer
8th Annual Meeting of Molecular Neurosurgery
Place of Presentation
Nishinomiya
Description
「研究成果報告書概要(欧文)」より
Related Report
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