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Microbial mechanism for sustainable nitrogen fertility in rice paddy soil

Research Project

Project/Area Number 17H01464
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Senoo Keishi  東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)

Co-Investigator(Kenkyū-buntansha) 早川 智恵  宇都宮大学, 農学部, 助教 (10725526)
豊田 栄  東京工業大学, 物質理工学院, 准教授 (30313357)
磯部 一夫  東京大学, 大学院農学生命科学研究科(農学部), 助教 (30621833)
服部 祥平  東京工業大学, 物質理工学院, 助教 (70700152)
伊藤 英臣  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (70748425)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2019: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2018: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2017: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Keywords水田土壌 / 窒素肥沃度 / 窒素固定 / DNRA / 鉄還元菌 / 鉄還元細菌 / 還元的窒素変換反応 / 土壌微生物
Outline of Final Research Achievements

We examined microbial mechanisms for sustainable nitrogen fertility in rice paddy soil. Soil analyses indicated that nitrogen fixation and DNRA (dissimilatory nitrate reduction to ammonia) driven by iron-reducing bacteria are important for the sustainable soil nitrogen fertility. Indeed, we verified nitrogen-fixing ability of iron-reducing bacteria isolated from paddy soils. In addition, application of iron to paddy soil could enhance nitrogen fixing activity of the soils. On the other hand, we found a Bradyrhizobium strain that might contribute to sustainable nitrogen fertility by fixing N2O, one of the end products of denitrification in paddy soil.

Academic Significance and Societal Importance of the Research Achievements

「稲は地力でとる」と言われる、水田土壌が本来有する窒素肥沃度維持につながる微生物メカニズムとして、鉄還元菌が行う窒素固定ならびにアンモニア生成反応が重要であることを世界で初めて明らかにした。窒素肥沃度維持の微生物基盤を刷新するものであり、水田土壌微生物学の大きな前進である。鉄の施用による土壌窒素固定活性増強の発見は、窒素肥料を低減した水稲生産技術につながり、地球・地域環境を保全した農業に大きく貢献する。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (25 results)

All 2021 2020 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 9 results,  Invited: 6 results)

  • [Int'l Joint Research] Nanjing Univ. Information Sci. Tech./Nanjing Tech University/Xi'an University of Technology(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Geomonas silvestris sp. nov., Geomonas paludis sp. nov. and Geomonas limicola sp. nov., isolated from terrestrial environments, and emended description of the genus Geomonas2021

    • Author(s)
      Itoh Hideomi、Xu Zhenxing、Masuda Yoko、Ushijima Natsumi、Hayakawa Chie、Shiratori Yutaka、Senoo Keishi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 71 Issue: 1

    • DOI

      10.1099/ijsem.0.004607

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of the nitrogen-fixing activity of paddy soils owing to iron application2021

    • Author(s)
      Masuda Yoko、Shiratori Yutaka、Ohba Hirotomo、Ishida Takanori、Takano Ryo、Satoh Sakura、Shen Weishou、Gao Nan、Itoh Hideomi、Senoo Keishi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 1 Issue: 3 Pages: 1-5

    • DOI

      10.1080/00380768.2021.1888629

    • NAID

      210000158861

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DiazotrophicAnaeromyxobacterIsolates from Soils2020

    • Author(s)
      Masuda Yoko、Yamanaka Haruka、Xu Zhen-Xing、Shiratori Yutaka、Aono Toshihiro、Amachi Seigo、Senoo Keishi、Itoh Hideomi
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 86 Issue: 16

    • DOI

      10.1128/aem.00956-20

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Description of Three Novel Members in the Family Geobacteraceae, Oryzomonas japonicum gen. nov., sp. nov., Oryzomonas sagensis sp. nov., and Oryzomonas ruber sp. nov.2020

    • Author(s)
      Xu Zhenxing、Masuda Yoko、Hayakawa Chie、Ushijima Natsumi、Kawano Keisuke、Shiratori Yutaka、Senoo Keishi、Itoh Hideomi
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 5 Pages: 634-634

    • DOI

      10.3390/microorganisms8050634

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predominant but Previously-overlooked Prokaryotic Drivers of Reductive Nitrogen Transformation in Paddy Soils, Revealed by Metatranscriptomics2017

    • Author(s)
      Masuda Y, Itoh H, Shiratori Y, Isobe K, Otsuka S, Senoo K.
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 180-183

    • DOI

      10.1264/jsme2.ME16179

    • NAID

      130006789873

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 増田曜子、石田敬典、山中遥加、Zhenxing Xu、伊藤英臣、白鳥豊、大峡広智、妹尾啓史2020

    • Author(s)
      水田土壌における鉄還元菌窒素固定の発見・検証と鉄・稲わら添加による増強
    • Organizer
      日本土壌肥料学会2020年度岡山大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 増田曜子、石田敬典、伊藤英臣、早川智恵、白鳥豊、妹尾啓史2020

    • Author(s)
      水田土壌の鉄還元菌窒素固定の増強-稲わらと鉄添加の効果
    • Organizer
      日本土壌微生物学会2020年度大会(大阪)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification and isolation of active N2O reducers in rice paddy soil, and application to N2O mitigation2018

    • Author(s)
      Keishi Senoo
    • Organizer
      China-Japan Symposium on Rhizosphere Cross-talk (NFSC-JST Cooperative Research Projects)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microbial Drivers of Nitrogen Transformation in Rice Paddy Soil: Identification, Isolation and Application to N2O Mitigation2018

    • Author(s)
      Keishi Senoo
    • Organizer
      Seminar at Nanjing University of Information Science and Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microbial Drivers of Nitrogen Transformation in Rice Paddy Soil: Identification, Isolation and Application to N2O Mitigation2018

    • Author(s)
      Keishi Senoo
    • Organizer
      Seminar at Nanjing Agricultural University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microbial Ecology of Nitrogen Dynamics in Agricultural Soils: Key Drivers of Reductive Nitrogen Transformation in Paddy Field and N2O Generation in Upland Field2018

    • Author(s)
      Keishi SENOO
    • Organizer
      2018 International Symposium and Annual Meeting of the KSABC
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nitrogen fixing activity of iron reducing bacteria in paddy soils: potent agents for low nitrogen rice production in East Asia2018

    • Author(s)
      Yoko Masuda, Hideomi Itoh, Yutaka Shiratori, Keishi Senoo
    • Organizer
      NARO-MARCO International Symposium on Nitrogen Cycling and Its Environmental Impacts in East Asia
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 農耕地土壌の窒素循環微生物の群集構造と機能、環境保全型農業への応用2018

    • Author(s)
      妹尾啓史
    • Organizer
      (一財)バイオインダストリー協会 植物バイオ研究会 第16回会合「植物バイオの先端技術(1)セミナー」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 陸域土壌における鉄還元菌窒素固定遺伝子の分布-水田だけではない!?鉄還元菌による窒素固定-2018

    • Author(s)
      増田曜子、伊藤英臣、白鳥豊、天知誠吾、妹尾啓史
    • Organizer
      日本微生物生態学会、10th Asian Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 鉄還元細菌の窒素固定能の検証2018

    • Author(s)
      山中遥加、増田曜子、伊藤英臣、青野俊裕、天知誠吾、白鳥豊、妹尾啓史
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 還元的な陸域環境下における鉄還元細菌の窒素固定活性の検証2018

    • Author(s)
      松島賢吾、藤村玲子、増田曜子、山中遥加、伊藤英臣、白鳥豊、青野俊裕、大塚重人、妹尾啓史
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水田土壌の優占種、鉄還元細菌の新機能 ~窒素肥沃度を支えるキープレーヤー~2018

    • Author(s)
      増田曜子、伊藤英臣、白鳥豊、妹尾啓史
    • Organizer
      第11回北陸バイオシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Isolation and characterization of Geobacter species, potential drivers of reductive nitrogen transformation in paddy soils2018

    • Author(s)
      Zhenxing Xu, Yoko Masuda, Hideomi Itoh, Keishi Senoo
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Microbial drivers of nitrogen transformation in rice paddy soil: Identification, isolation, and application to N2O mitigataion2017

    • Author(s)
      Keishi Senoo
    • Organizer
      Seminar at Jiangsu University
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Predominant but previously-unseen prokaryotic drivers of reductive nitrogen transformation in paddy soils, unveiled by metatranscriptomics2017

    • Author(s)
      Keishi Senoo
    • Organizer
      US-Japan Joint Seminar on Microbial Biotechnology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Predominant but previously-overlooked diazotrophs in wetland rice soils, unveiled by metatranscriptomics2017

    • Author(s)
      Yoko Masuda, Hideomi Itoh, Yutaka Shiratori, Keishi Senoo
    • Organizer
      20th International Congress on Nitrogen Fixation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水田土壌オミクス解析から見えた陸域生態系における鉄還元菌の新たな機能-還元的窒素変換2017

    • Author(s)
      増田曜子、白鳥豊、磯部一夫、大塚重人、妹尾啓史
    • Organizer
      日本地球惑星連合2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水田土壌の鉄還元菌のもう一つの顔:窒素肥沃度を支えるキープレーヤー2017

    • Author(s)
      増田曜子、妹尾啓史、伊藤英臣、白鳥豊
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水田土壌微生物の優占種、鉄還元菌の窒素肥沃度維持への寄与の可能性―オミクス解析から見えた鉄還元菌が駆動する窒素固定、DNRA、脱窒―2017

    • Author(s)
      増田曜子、伊藤英臣、白鳥 豊、石井勝博、磯部一夫、藤村玲子、早川智恵、大塚重人、妹尾啓史
    • Organizer
      日本土壌肥料学会2017年度仙台大会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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