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Methane fermentation of inedible biomass by highly concentrated and compacted functional rumen microbial communities

Research Project

Project/Area Number 17H01512
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNiigata Agro-Food University (2018-2020)
Tohoku University (2017)

Principal Investigator

Yutaka Nakai  新潟食料農業大学, 食料産業学科, 教授 (80155655)

Co-Investigator(Kenkyū-buntansha) 多田 千佳  東北大学, 農学研究科, 准教授 (30413892)
福田 康弘  東北大学, 農学研究科, 助教 (50527794)
馬場 保徳  石川県立大学, 生物資源環境学部, 講師 (70747823)
阿部 憲一  新潟食料農業大学, 食料産業学科, 講師 (80618835)
浅野 亮樹  新潟食料農業大学, 食料産業学科, 講師 (20646137)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2020: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2019: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2018: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2017: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywordsメタン / ルーメン微生物 / バイオマス / メタゲノム / セルラーゼ / リグノセルロース / キシラン / メタン発酵
Outline of Final Research Achievements

After the treatment of crop residues or waste paper by cattle rumen fluid, methane fermentation of these materials was accelerated. Enzymes and microbial communities in the process was investigated. Fiber‐degrading cellulase was diverse in the process; however, specific enzymes of xylanase showed high enzymatic activity continuously. Cellulase activity in the rumen treatment tank was continued for more than one month. At least five types of cellulases having different molecular sizes were existed. Microbes producing or involving these cellulases were unclassified species and not dominant in the microbial community.

Academic Significance and Societal Importance of the Research Achievements

申請者らは牛のルーメン(第一胃)液を活用したルーメン・ハイブリッド型メタン発酵システムを開発し、草本のリグノセルロース分解効率を高め、高効率なバイオガス化を実現してきた。さらに本研究により、ルーメン微生物の植物繊維分解の活性を長期間維持できること、さらにこれらに関わる酵素の種類、構造などをあきらかにした。以上より、本研究は非食用バイオマスのエネルギー化システム開発に必要な重要な情報を提供した。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (23 results)

All 2020 2019 2018 2017

All Journal Article (10 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Change of Endoglucanase Activity and Rumen Microbial Community During Biodegradation of Cellulose Using Rumen Microbiota2020

    • Author(s)
      Takizawa Shuhei、Asano Ryoki、Fukuda Yasuhiro、Feng Mengjia、Baba Yasunori、Abe Kenichi、Tada Chika、Nakai Yutaka
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 603818-603818

    • DOI

      10.3389/fmicb.2020.603818

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of bacteria involved in the decomposition of lignocellulosic biomass treated with cow rumen fluid by metagenomic analysis2020

    • Author(s)
      Lee Chol Gyu、Baba Yasunori、Asano Ryoki、Fukuda Yasuhiro、Tada Chika、Nakai Yutaka
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 130 Issue: 2 Pages: 137-141

    • DOI

      10.1016/j.jbiosc.2020.03.010

    • NAID

      40022324657

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 牛ルーメン微生物を用いた高効率バイオガス生産技術の開発2020

    • Author(s)
      馬場保徳
    • Journal Title

      JATAFFジャーナル

      Volume: 8 Pages: 18-24

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Recovery of the fibrolytic microorganisms from rumen fluid by flocculation for simultaneous treatment of lignocellulosic biomass and volatile fatty acid production2020

    • Author(s)
      Takizawa Shuhei、Abe Kenichi、Fukuda Yasuhiro、Feng Mengjia、Baba Yasunori、Tada Chika、Nakai Yutaka
    • Journal Title

      Journal of Cleaner Production

      Volume: 257 Pages: 120626-120626

    • DOI

      10.1016/j.jclepro.2020.120626

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition.2020

    • Author(s)
      Shuhei Takizawa,Yasunori Baba,Chika Tada,Yasuhiro Fukuda &Yutaka Nakai
    • Journal Title

      Journal of Material Cycles and Waste Management,

      Volume: 22 Pages: 656-663

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in Bacterial Communities in Seawater-Flooded Soil in the Four Years After the 2011 Tohoku Tsunami in Japan2020

    • Author(s)
      Asano Ryoki、Hayakawa Atsushi、Fukushima Jun、Nakai Yutaka、Shimura Yoichiro、Abe Midori、Inamoto Tamio
    • Journal Title

      Journal of Marine Science and Engineering

      Volume: 8 Issue: 2 Pages: 76-76

    • DOI

      10.3390/jmse8020076

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pretreatment of Lignocellulosic Biomass with Cattle Rumen Fluid for Methane Production: Fate of Added Rumen Microbes and Indigenous Microbes of Methane Seed Sludge2019

    • Author(s)
      Baba Yasunori、Matsuki Yu、Takizawa Shuhei、Suyama Yoshihisa、Tada Chika、Fukuda Yasuhiro、Saito Masanori、Nakai Yutaka
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 4 Pages: 421-428

    • DOI

      10.1264/jsme2.ME19113

    • NAID

      130007776137

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preservation of rumen fluid for the pretreatment of waste paper to improve methane production2019

    • Author(s)
      Takizawa Shuhei、Baba Yasunori、Tada Chika、Fukuda Yasuhiro、Nakai Yutaka
    • Journal Title

      Waste Management

      Volume: 87 Pages: 672-678

    • DOI

      10.1016/j.wasman.2019.02.043

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pretreatment with rumen fluid improves methane production in the anaerobic digestion of paper sludge2018

    • Author(s)
      Takizawa Shuhei、Baba Yasunori、Tada Chika、Fukuda Yasuhiro、Nakai Yutaka
    • Journal Title

      Waste Management

      Volume: 78 Pages: 379-384

    • DOI

      10.1016/j.wasman.2018.05.046

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 東日本大震災で津波浸水した農地土壌の5年後における微生物群集解析.2017

    • Author(s)
      浅野亮樹,早川敦,阿部みどり,志村洋一郎,小林弥生,福島淳.
    • Journal Title

      秋田県立大学ウェブジャーナル.

      Volume: 4 Pages: 147-152

    • NAID

      120006354896

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Presentation] 凝結凝集法によって回収したルーメン微生物のエンドグルカナーゼ活性の特徴2019

    • Author(s)
      瀧澤修平, 阿部憲一, 福田康弘, 多田千佳, 中井裕.
    • Organizer
      . 第18回 日本畜産環境学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 凝結凝集法がルーメン微生物の回収効率および植物系バイオマス分解特性におよぼす影響.2019

    • Author(s)
      瀧澤修平, 阿部憲一, 福田康弘, 多田千佳, 中井裕
    • Organizer
      第30回 廃棄物資源循環学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fibrolytic Enzyme Activity During Pretreatment using Rumen Fluid for the Anaerobic Digestion of Lignocellulose.2019

    • Author(s)
      S. Takizawa, Y. Fukuda, C. Tada, Y. Nakai.
    • Organizer
      16th International Water Association World Conference on Anaerobic Digestion.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ルーメン液を利用したペーパースラッジのバイオガス化におけるルーメン原虫の影響2018

    • Author(s)
      瀧澤修平,多田千佳,福田康弘,中井裕
    • Organizer
      第12回バイオマス科学会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] ルーメン液を利用した植物系バイオマスの前処理におけるセルラーゼの挙動2018

    • Author(s)
      瀧澤修平,福田康弘,多田千佳,中井裕
    • Organizer
      第17回日本畜産環境学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ルーメン微生物群集による植物系バイオマス分解過程における多糖分解酵素活性の推移2018

    • Author(s)
      瀧澤修平,浅野亮樹,福田康弘,多田千佳,中井裕
    • Organizer
      第125回日本畜産学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Change in Cellulase Activity during Pretreatment with Rumen Fluid for the Anaerobic Digestion of Lignocellulosic Biomass2018

    • Author(s)
      Takizawa S, Fukuda Y, Tada C, Nakai Y
    • Organizer
      Water and Environment Technology Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Continuous solubilization of organic waste by cattle rumen fluid for methane production2018

    • Author(s)
      Adachi, H., Goto, N., Kusube, T., Kawai, S., Baba, Y
    • Organizer
      2018 Joint Seminar on Environmental Ecology and Restoration between Taiwan and Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メタン発酵効率化のためのルーメン微生物を利用した植物バイオマスの連続可溶化2018

    • Author(s)
      足立明香, 藤暢宏, 楠部孝誠, 河井重幸, 馬場保徳.
    • Organizer
      第11回北陸合同バイオシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシルーメン液処理によるメタン発酵効率化 ;複合微生物系の理解に向けた NGSの活用2018

    • Author(s)
      馬場保徳
    • Organizer
      日本生物工学会バイオインフォマティクス相談部会講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ウシルーメン液によるメタン発酵前処理過程の微生物群集構造解析.2017

    • Author(s)
      馬場保徳,松木悠,馬場(森)裕美,陶山佳久,多田千佳,福田康弘,三宅克英,齋藤雅典,中井裕.
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウシルーメン液による植物バイオマス可溶化処理およびその後のメタン発酵過程の微生物群集構造解析.2017

    • Author(s)
      馬場保徳,松木悠,馬場(森)裕美,陶山佳久,多田千佳,福田康弘,三宅克英,齋藤雅典,中井裕.
    • Organizer
      第2回環境微生物系学会合同大会
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] Method of organic acid fermentation by rumen fluid using cellulose-containing waste matter2019

    • Inventor(s)
      中井裕,馬場保徳,多田千佳,福田康弘
    • Industrial Property Rights Holder
      中井裕,馬場保徳,多田千佳,福田康弘
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Acquisition Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas

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Published: 2017-04-28   Modified: 2022-01-27  

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