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Neural tissue array with blood brain barrier (BBB) for the drug screening assay

Research Project

Project/Area Number 17H03197
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionMusashino University

Principal Investigator

Negishi Midori  武蔵野大学, 薬学部, 助教 (30300750)

Co-Investigator(Kenkyū-buntansha) 尾上 弘晃  慶應義塾大学, 理工学部(矢上), 教授 (30548681)
川原 正博  武蔵野大学, 薬学部, 教授 (40224828)
森本 雄矢  東京大学, 大学院情報理工学系研究科, 准教授 (60739233)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywordsマイクロデバイス / 組織チップ / 薬剤スクリーニング / 神経科学 / ドラッグスクリーニング / 組織工学 / 再生医療 / 薬物スクリーニング
Outline of Final Research Achievements

We succeeded in fabricating a 3D tissue array suitable for drug screening to analyze the effects of a vast array of drugs on neural tissue in a short time. The 3D tissue arrays constructed using nerve tissue with a tubular-like structure had the following characteristics: no cell death was observed at the tissue center, the number of cells was uniform, there were enough cells for the assay, and the shape was uniform. The drug assay using this 3D tissue array was able to pass the acceptance criteria for drug screening. It was also suggested that the assay could be used to evaluate cell proliferation as well as cell death. We also established a perfusion culture system for neural tissue and vascular endothelial cells. In the future, this system will be used to search for novel drugs for neural diseases.

Academic Significance and Societal Importance of the Research Achievements

2次元培養と組織では薬物の効果が異なることが報告されており、ドラッグスクリーニングに適した3次元組織アレイの開発が求められていた。これまで3次元組織アレイとしては、スフェロイドを利用した抗がん剤のスクリーニングが主流であったが、本研究で開発した3次元組織アレイは、薬剤スクリーニングの受け入れ基準をクリアし、細胞死や細胞増殖の評価を行うことができる新規の組織アレイである。神経組織アレイと血管内皮の共培養系を利用することで、血液中からの組織への薬物取り込みを評価することが可能となり、将来的には大量の薬物スクリーニングに利用できる可能性があると考えられる。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (12 results)

All 2022 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Book (2 results)

  • [Journal Article] Cell fiber-based 3D tissue array for drug response assay2022

    • Author(s)
      Midori Kato-Negishi, Jun Sawayama, Masahiro Kawahara & Shoji Takeuchi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 7870-7870

    • DOI

      10.1038/s41598-022-11670-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neurotoxicity of aluminum and its link to neurodegenerative diseases2021

    • Author(s)
      Masahiro Kawahara, Ken-ichiro Tanaka, Midori Kato-Negishi
    • Journal Title

      Metallomics Research

      Volume: 1 Issue: 1 Pages: rev-47-rev-65

    • DOI

      10.11299/metallomicsresearch.MR202104

    • NAID

      130008145805

    • ISSN
      2436-5173
    • Year and Date
      2021-12-15
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Copper as a Collaborative Partner of Zinc-Induced Neurotoxicity in the Pathogenesis of Vascular Dementia2021

    • Author(s)
      Kawahara Masahiro、Tanaka Ken-ichiro、Kato-Negishi Midori
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 14 Pages: 7242-7242

    • DOI

      10.3390/ijms22147242

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Amyloids: Regulators of Metal Homeostasis in the Synapse2020

    • Author(s)
      Kawahara M, Kato-Negishi M, Tanaka KI
    • Journal Title

      Molecules

      Volume: 25 Issue: 6 Pages: 1441-1441

    • DOI

      10.3390/molecules25061441

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Carnosine as a Possible Drug for Zinc-Induced Neurotoxicity and Vascular Dementia.2020

    • Author(s)
      Kawahara M, Sadakane Y, Mizuno K, Kato-Negishi M, Tanaka KI.
    • Journal Title

      Int J Mol Sci.

      Volume: 21 Issue: 7 Pages: 2570-2570

    • DOI

      10.3390/ijms21072570

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 機能的な3次元神経組織構築のための細胞ファイバ技術2021

    • Author(s)
      根岸みどり,竹内昌治
    • Organizer
      第44回日本神経科学大会 / CJK第1回国際会議
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ハイスループットスクリーニングのための3次元組織アレイ2020

    • Author(s)
      根岸 みどり、澤山 淳、川原 正博、竹内 昌治
    • Organizer
      日本薬学会第140年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] FIiber-shaped 3D tissue in a 96 well plate for high-throughput drug screening2019

    • Author(s)
      Midori Kato-Negishi, Jun Sawayama, and Shoji Takeuchi
    • Organizer
      23rd International Conference on Miniaturized Systems for Chemistry and Life Science
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of coaxial hierarchical-layered cell-laden fiber2018

    • Author(s)
      Y. Morimoto, M. Kiyosawa, M. Kato-Negishi, S. Takeuchi
    • Organizer
      22th MicroTAS 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経幹細胞を利用したドラッグスクリーニングのための3次元神経組織構築2018

    • Author(s)
      根岸みどり、松代悠暉、尾上弘晃
    • Organizer
      日本薬学会第138年回
    • Related Report
      2017 Annual Research Report
  • [Book] 脳血管性認知症発症における亜鉛と銅のクロストーク2019

    • Author(s)
      川原正博、根岸みどり、田中健一郎
    • Total Pages
      10
    • Publisher
      亜鉛栄養治療
    • Related Report
      2019 Annual Research Report
  • [Book] 再生医療・創薬のための3次元細胞培養技術2018

    • Author(s)
      根岸みどり, 森本雄矢, 竹内昌治
    • Total Pages
      203
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313306
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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