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Identification of host-specific viral genetic markers for fecal contamination analysis of environmental water

Research Project

Project/Area Number 17H03332
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionUniversity of Yamanashi

Principal Investigator

HARAMOTO Eiji  山梨大学, 大学院総合研究部, 教授 (00401141)

Co-Investigator(Kenkyū-buntansha) 北島 正章  北海道大学, 工学研究院, 助教 (30777967)
端 昭彦  富山県立大学, 工学部, 講師 (70726306)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywordsウイルス / ファージ / 糞便汚染源解析 / 公共用水域 / 微生物起源解析
Outline of Final Research Achievements

This study aimed to identify host-specific viral genetic markers which can be used for fecal contamination analysis of the aquatic environments. Various types of fecal-source samples were used to find suitable viral genetic markers in the tested area. Furthermore, the selected markers were successfully used to determine the situations of fecal contamination of river water samples with different characteristics.

Academic Significance and Societal Importance of the Research Achievements

現在,環境省により水質環境基準における衛生指標を大腸菌群から大腸菌に変更することが検討されているが,大腸菌を測定するのみでは糞便汚染源となる宿主に関する情報を得ることはできない。本研究の成果は,宿主特異性の高いウイルスやファージを遺伝子マーカーとして用いることにより,これまでは明らかにされてこなかった水環境中の糞便汚染源に関する知見を得ることを可能とするものであり,世界的に見ても非常に高い学術的成果が得られている。
本研究の成果は,水環境中の糞便汚染源を特定し,適切な糞便汚染負荷低減策を採ることを可能とするものであり,健全な水環境の構築に貢献できるものであり,社会的意義も高いと言える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (18 results) (of which Int'l Joint Research: 6 results)

  • [Int'l Joint Research] トリブバン大学(ネパール)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] テュレーン大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Applicability of crAssphage, pepper mild mottle virus, and tobacco mosaic virus as indicators of reduction of enteric viruses during wastewater treatment2020

    • Author(s)
      Tandukar Sarmila、Sherchan Samendra P.、Haramoto Eiji
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 3616-3616

    • DOI

      10.1038/s41598-020-60547-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Host-specific mitochondrial DNA markers for tracking the sources of fecal pollution2020

    • Author(s)
      Malla Bikash、Haramoto Eiji
    • Journal Title

      Current Opinion in Environmental Science & Health

      Volume: 16 Pages: 34-46

    • DOI

      10.1016/j.coesh.2020.02.006

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Pathogenic Viruses, Pathogen Indicators, and Fecal-Source Markers within Tanker Water and Their Sources in the Kathmandu Valley, Nepal2019

    • Author(s)
      Malla Bikash、Ghaju Shrestha Rajani、Tandukar Sarmila、Bhandari Dinesh、Thakali Ocean、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Pathogens

      Volume: 8 Issue: 2 Pages: 81-81

    • DOI

      10.3390/pathogens8020081

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Performance Evaluation of Human-Specific Viral Markers and Application of Pepper Mild Mottle Virus and CrAssphage to Environmental Water Samples as Fecal Pollution Markers in the Kathmandu Valley, Nepal2019

    • Author(s)
      Malla Bikash、Ghaju Shrestha Rajani、Tandukar Sarmila、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Food and Environmental Virology

      Volume: 11 Issue: 3 Pages: 274-287

    • DOI

      10.1007/s12560-019-09389-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evaluation of Human- and Animal-Specific Viral Markers and Application of CrAssphage, Pepper Mild Mottle Virus, and Tobacco Mosaic Virus as Potential Fecal Pollution Markers to River Water in Japan2019

    • Author(s)
      Malla Bikash、Makise Koki、Nakaya Koki、Mochizuki Taizo、Yamada Takahiro、Haramoto Eiji
    • Journal Title

      Food and Environmental Virology

      Volume: 11 Issue: 4 Pages: 446-452

    • DOI

      10.1007/s12560-019-09398-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Presence of human enteric viruses, protozoa, and indicators of pathogens in the Bagmati River, Nepal2018

    • Author(s)
      Sarmila Tandukar, Jeevan B. Sherchand, Dinesh Bhandari, Samendra P. Sherchan, Bikash Malla, Rajani Ghaju Shrestha, Eiji Haramoto
    • Journal Title

      Pathogens

      Volume: 7 Issue: 2 Pages: 38-38

    • DOI

      10.3390/pathogens7020038

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A review on recent progress in the detection methods and prevalence of human enteric viruses in water2018

    • Author(s)
      Eiji Haramoto, Masaaki Kitajima, Akihiko Hata, Jason R. Torrey, Yoshifumi Masago, Daisuke Sano, Hiroyuki Katayama
    • Journal Title

      Water Research

      Volume: 135 Pages: 168-186

    • DOI

      10.1016/j.watres.2018.02.004

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters2018

    • Author(s)
      Akihiko Hata, Masaaki Kitajima, Eiji Haramoto, Suntae Lee, Masaru Ihara, Charles P. Gerba, Hiroaki Tanaka
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11837-11837

    • DOI

      10.1038/s41598-018-30217-y

    • NAID

      120006529727

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pepper mild mottle virus as a water quality indicator2018

    • Author(s)
      M. Kitajima, H. P. Sassi, J. R. Torrey
    • Journal Title

      npj Clean Water

      Volume: 1 Issue: 1 Pages: 19-19

    • DOI

      10.1038/s41545-018-0019-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Virological quality of irrigation water sources and pepper mild mottle virus and tobacco mosaic virus as index of pathogenic virus contamination level2017

    • Author(s)
      Sadhana Shrestha, Shankar Shrestha, Junko Shindo, Jeevan B. Sherchand, Eiji Haramoto
    • Journal Title

      Food and Environmental Virology

      Volume: 10 Issue: 1 Pages: 107-120

    • DOI

      10.1007/s12560-017-9324-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Enteric virus contamination in hospital wastewater of the Kathmandu Valley, Nepal2019

    • Author(s)
      Sarmila Tandukar, Jeevan B. Sherchand, Bikash Malla, Rajani Ghaju Shrestha, Ocean Thakali, Eiji Haramoto
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 河川水中の糞便汚染指標としての体表面吸着大腸菌ファージの有効性評価2019

    • Author(s)
      伊藤聖晃, 原本英司
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Performance evaluation of animal-specific viral and mitochondrial DNA markers and their application to microbial source tracking of drinking water sources in the Kathmandu Valley, Nepal2019

    • Author(s)
      Bikash Malla, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of applicability of somatic coliphages as indicators of fecal contamination in river water2019

    • Author(s)
      Masaaki Ito, Taizo Mochizuki, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reduction of enteropathogens at two conventional wastewater treatment plants in Nepal2019

    • Author(s)
      Sarmila Tandukar, Jeevan B. Sherchand, Bikash Malla, Rajani Ghaju Shrestha, Ocean Thakali, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reduction of crAssphage and enteric viruses during conventional wastewater treatment2019

    • Author(s)
      Samendra Sherchan, Sarmila Tandukar, Eiji Haramoto
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pepper mild mottle virus and crAssphage as fecal pollution markers in aquatic environments of the Kathmandu Valley, Nepal2019

    • Author(s)
      Bikash Malla, Rajani Ghaju Shrestha, Sarmila Tandukar, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 塩素消毒処理による大腸菌ファージ野生株の遺伝子型別の不活化効果2019

    • Author(s)
      堂山貴広, 原本英司
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 河川水中の糞便汚染指標としてのCrAssphageの有効性の検討2019

    • Author(s)
      牧瀬晃基, Bikash Malla, 原本英司, 坂本康
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 環境水中における体表面吸着大腸菌ファージの糞便汚染指標としての有効性評価2019

    • Author(s)
      伊藤聖晃, 望月大蔵, 原本英司
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 河川水中におけるF特異DNA大腸菌ファージの存在実態および糞便汚染源指標としての有効性の検討2019

    • Author(s)
      古賀皓晟, 堂山貴広, 原本英司, 坂本康
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ミトコンドリアDNAおよびバクテロイデス遺伝子マーカーを用いた河川水中の糞便汚染源解析2019

    • Author(s)
      山本一輝, Bikash Malla, 原本英司, 坂本康
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 宿主特異的微生物遺伝子マーカーを用いた水環境中の糞便汚染源解析2018

    • Author(s)
      原本英司
    • Organizer
      第21回日本水環境学会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Presence of enteric viruses and protozoa in different sources of water in the Kathmandu Valley, Nepal2018

    • Author(s)
      Sarmila Tandukar, Bikash Malla, Rajani Ghaju Shrestha, Dinesh Bhandari, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      ASM Microbe 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 宿主特異的ウイルス遺伝子マーカーを用いた甲府盆地河川水中の糞便汚染源の解析2018

    • Author(s)
      中谷航己, 山田貴大, Bikash Malla, 原本英司
    • Organizer
      第45回土木学会関東支部技術研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] F特異RNA大腸菌ファージ野生株の遺伝子群別の塩素消毒耐性2018

    • Author(s)
      堂山貴広, 原本英司
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 河川水中の糞便汚染指標としての体表面吸着大腸菌ファージの遺伝子群別検出の有効性2018

    • Author(s)
      望月大蔵, 原本英司
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 下水および河川水中におけるF特異大腸菌ファージの遺伝子群別解析2017

    • Author(s)
      堂山貴広, 望月大蔵, 原本英司
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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