Demonstration of integrated microfluidic system that realizes selection / placement / 3-dimensional complex of cells
| Project/Area Number |
17H03463
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Chiba University |
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Principal Investigator |
Seki Minoru 千葉大学, 大学院工学研究院, 教授 (80206622)
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| Co-Investigator(Kenkyū-buntansha) |
山田 真澄 千葉大学, 大学院工学研究院, 准教授 (30546784)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | マイクロ・ナノデバイス / 細胞選抜 / 3次元組織 / 細胞外マトリックス / 細胞アッセイ / マイクロナノデバイス |
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| Outline of Final Research Achievements |
In constructing a three-dimensional tissue model by assembling individual cells, the technology of selecting the required cells, accurately arranging multiple types of cells, and fixing them in a three-dimensional space is essential.
In this study, we developed a microfluidic device that automatically and efficiently achieves these individual operations, demonstrated its usefulness, and developed a connected system that integrates them. Then, we actually demonstrated the production of composite liver tissues and multi-layered blood vessel models.
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| Academic Significance and Societal Importance of the Research Achievements |
通常の手作業の実験系では不可能な，精密な細胞操作を可能とする新規システムの開発を行うことができた。特に細胞の選抜においては，高効率な新規マイクロ流体デバイスを提案でき，また細胞の配置と組織化については，細胞親和性のあるハイドロゲル材料に細胞を生きたまま導入し，その機能を向上できることが確認された。これらの手法は，再生医療や創薬のみならず，診断医療や基礎生物学にも役立つ基盤技術になりえるものである。
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Report
(4 results)
Research Products
(65 results)
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[Presentation] Separated Co-culture of Heterotypic Cells Using Microfabricated Thin ECM Membranes2018
Author(s)
Hideki Iwadate, Naoki Kimura, Rina Hashimoto, Yuya Yajima, Rie Utoh, Masumi Yamada, and Minoru Seki
Organizer
29th 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS 2018), Nagoya University, Japan, Dec. 9-12, 2018
Related Report
Int'l Joint Research
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[Presentation] Wrapping of Linear Cell Assemblies with Tubular Collagen Membranes Using Multilayered Microfluidic Devices2018
Author(s)
Kotone Saeki, Masumi Yamada, Sakiko Enomoto, Yuya Yajima, Rie Utoh, and Minoru Seki
Organizer
The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Nov. 11-15, 2018
Related Report
Int'l Joint Research
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[Presentation] Invasion Assay of Cancer Cells Using Hierarchically-patterned Hydrogel Sheets2017
Author(s)
Manami Sugimoto, Yoichi Kitagawa, Yuya Yajima, Rie Utoh, Masumi Yamada, and Minoru Seki
Organizer
RSC Tokyo International Conference 2017 - Analytical Technology Towards Future Society - (JASIS 2017), Makuhari Messe, Chiba, Japan, Sep. 7-8, 2017
Related Report
Int'l Joint Research
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[Presentation] Collagen Gelation on Phosphate Particle-embedding PDMS (PP-PDMS) Channel: An Efficient Approach to Vascular Tissue Engineering2017
Author(s)
Mayu Fukushi, Yuya Yajima, Rie Utoh, Masumi Yamada, Kazuya Furusawa and Minoru Seki
Organizer
The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), Savannah International Trade & Convention Center, Savannah, GA, USA, Oct. 22-26, 2017
Related Report
Int'l Joint Research
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