Study on noncoding RNA-dependent architecture of nuclear 3D structure
| Project/Area Number |
17H03630
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
Hirose Tetsuro 北海道大学, 遺伝子病制御研究所, 教授 (30273220)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
|---|
| Keywords | noncoding RNA / 相分離 / 核内構造体 / クロマチン / 遺伝子発現制御 / RNA / エピジェネティクス / クロマチン動態 / 核構造・機能 / 超分子複合体 / ノンコーディングRNA / タンパク質 / 遺伝子 / 核酸 / ゲノム / 細胞・組織 / 蛋白質 / 核内構造 / エピジェネティック制御 |
|---|
| Outline of Final Research Achievements |
Our research revealed that nuclear body paraspeckle is formed through intracellular phase separation induced by NEAT1 lncRNA and play significant function in formation of 3D chromatin structure. Meanwhile thermal stress induced nuclear stress bodies formed with HSATIII lncRNA serve as the scaffold of temperature dependent phosphorylation of specific regulators for the regulation of pre-mRNA splicing.
|
| Academic Significance and Societal Importance of the Research Achievements |
混み合った細胞核内において、クロマチン構造や転写後遺伝子発現制御を効率よくかつ精密に制御するために、相分離した核内構造体が様々な役割を果たしていることが明らかになった。特にこの相分離空間が特定のncRNAを骨格にして形成されていることによって、核内の特定部位にそのRNAに親和性のあるタンパク質因子を集約することができ、さらにはその制御因子を介してクロマチン高次構造を規定したり、制御因子の翻訳後修飾の場として働くことを明らかにし、未だ明確ではない核内における相分離環境の意義を明確に示すことができた。
|
Report
(4 results)
Research Products
(70 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Long noncoding RNA NEAT1 modulates immune cell functions and is suppressed in early onset myocardial infarction patients.2019
Author(s)
Gast M, Rauch B, Haghikia A, Nakagawa S, Haas J, Stroux A, Schmidt D, Schumann P, Weiss S, Jensen L, Kratzer A, Kraenkel N, Mller C, B;rnigen D, Hirose T, Blankenberg S, Escher F, Khl A, Kuss A, Meder B, Landmesser U, Zeller T, Poller W.
-
Journal Title
Cardiovasc Res.
Volume: in press
Issue: 13
Pages: 1886-1906
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
[Journal Article] Long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1) is critical for phenotypic switching of vascular smooth muscle cells.2018
Author(s)
Ahmed ASI, Dong K, Liu J, Wen T, Yu L, Xu F, Kang X, Osman I, Hu G, Bunting KM, Crethers D, Gao H, Zhang W, Liu Y, Wen K, Agarwal G, Hirose T, Nakagawa S, Vazdarjanova A, Zhou J.
-
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 115
Issue: 37
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
[Journal Article] Neat1 is a p53-inducible lincRNA essential for transformation suppression.2017
Author(s)
Mello SS, Sinow C, Raj N, Mazur PK, Bieging-Rolett K, Broz DK, Imam JFC, Vogel H, Wood LD, Sage J, Hirose T, Nakagawa S, Rinn J, Attardi LD.
-
Journal Title
Genes Dev.
Volume: 31(11)
Issue: 11
Pages: 1095-1108
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Dissection of NEAT1 lncRNA domains to establish distinct, highly ordered, phase-separated paraspeckle nuclear body2018
Author(s)
山崎 智弘, Sylvie Souquere, 吉野 彪羅, 高桑 央, 木立 尚孝, Archa H. Fox, Charles S. Bond, 中川 真一, Gerard Pierron、廣瀬 哲郎
Organizer
Keystone symposium Noncoding RNAs: Form, Function, Physiology
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
