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Analysis of regulatory mechanisms of phototropism through protein phosphorylations

Research Project

Project/Area Number 17H03694
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNiigata University

Principal Investigator

Sakai Tatsuya  新潟大学, 自然科学系, 教授 (10360554)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords光屈性 / フォトトロピン / タンパク質リン酸化 / シグナル伝達 / 光寛容 / 光環境応答 / リン酸化解析 / 植物 / リン酸化修飾 / 光シグナル伝達 / 光シグナルリング
Outline of Final Research Achievements

Plant life is strongly dependent on light and Arabidopsis thaliana phototropin1 (phot1) is a blue-light photoreceptor, i.e. a blue-light-activated Ser/Thr-protein kinase that mediates various light responses including phototropism. Our research goal is an understanding of the phototropin signaling system. In this research, we were focusing on the protein phosphorylation of signaling factors in the phot1 signaling. Our study has revealed that RPT2 suppresses the phot1 autophosphorylation activity and that the phosphorylation status of NPH3 is involved in the photoadaptation mechanism of phototropism as an effector adaptation mechanism.

Academic Significance and Societal Importance of the Research Achievements

植物の芽生えは光照射側と陰側の光強度の差を認識し、光源方向に向けて地上部が成長する光屈性を示す。光屈性を誘導する光センサーシステムのダイナミックレンジは幅広いことが知られている。本研究で明らかになった知見は、タンパク質リン酸化調節を介したフォトトロピンシグナル伝達の強度調節であり、新たな光寛容システムの発見となった。本研究テーマは、光屈性におけるフォトトロピン分子機能の理解と発見を通じて、動くことができない植物が持っている柔軟かつ堅牢な光環境適応能力を学ぶともに、将来、分子デバイスとして光遺伝学等のバイオテクノロジー分野のツール開発に貢献することが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (8 results)

All 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Int'l Joint Research] University of Glasgow(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Glasgow(英国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Arabidopsis ROOT PHOTOTROPISM2 is a Light-Dependent Dynamic Modulator of Phototropin1.2020

    • Author(s)
      Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, and Tatsuya Sakai
    • Journal Title

      The Plant Cell

      Volume: 32 Issue: 6 Pages: 2004-2019

    • DOI

      10.1105/tpc.19.00926

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-fluence blue light-induced phosphorylation of Zmphot1 mediates the first positive phototropism.2019

    • Author(s)
      Hiromi Suzuki, Tomokazu Koshiba, Chiharu Fujita, Yoshio Yamauchi, Taro Kimura, Toshiaki Isobe, Tatsuya Sakai, Masato Taoka and Takashi Okamoto
    • Journal Title

      J. Exp. Bot.

      Volume: 70 Issue: 20 Pages: 5929-5941

    • DOI

      10.1093/jxb/erz344

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] De-etiolation Enhances Phototropism by Modulating NON-PHOTOTROPIC HYPOCOTYL 3 Phosphorylation Status2019

    • Author(s)
      Sullivan Stuart、Kharshiing Eros、Laird Janet、Sakai Tatsuya、Christie John
    • Journal Title

      Plant Physiology

      Volume: 印刷中 Issue: 2 Pages: 1119-1131

    • DOI

      10.1104/pp.19.00206

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナ黄化芽生え胚軸光屈性におけるNPH3脱リン酸化の意義2020

    • Author(s)
      木村太郎,芳賀健,野村有子,中神弘史,酒井達也
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 青色光に応じた葉柄運動におけるオーキシン輸送の多面的な役割2020

    • Author(s)
      大塚祐太,綿引雅昭,芳賀健,酒井達也,塚谷裕一
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナ胚軸光屈性において RPT2 が phot1 の光感受性を調節する仕組み2019

    • Author(s)
      木村太郎、間山(槌田)智子、酒井達也
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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