| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Outline of Final Research Achievements |
Gametocidal gene in wheat causes chromosome breakage in the gametophyte without itself and thus causing selective transmission of the chromosome carrying it. The objective of the study was to identify the gene through the map-based cloning approaches. Through various approaches of molecular genetics and genomics, we have successfully identified the candidate sequence. We have genetically proved that the candidate sequence encodes the chromosome-breaking function. We have tried to identify the tissues and stages of the wheat plant where the candidate gene is expressing, however, all our efforts of RNA in situ hybridization of the candidate sequence and immunostaining with the antibody raised against the whole protein sequence produced failed to identify its specificity. Through our analyses, we recognized that the other factor is required for full function of the candidate sequence.
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