Establishment of an efficient F1 breeding system based on cytoplasmic male sterility in tomato

• Project/Area Number: 17H03761
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticultural science
• Research Institution: University of Tsukuba
• Principal Investigator: Ariizumi Tohru 筑波大学, 生命環境系, 准教授 (70575381)
• Co-Investigator(Kenkyū-buntansha): 矢野 亮一 筑波大学, 生命環境系, 助教 (00443044)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: トマト / 細胞質雄性不稔生 / 花粉 / F1種子採種 / ミトコンドリア / 細胞質雄性不稔性 / 次世代シークエンサー / バイオインフォマティクス / 遺伝学 / 育種学 / 園芸学

Outline of Final Research Achievements

This study aimed to identify fertility restoring genes (RFs) for cytoplasmic male sterility (CMS) in tomato. First, we constructed a highly accurate genome sequence of two fertility restoration lines. The major loci contributing to fertility restoration were identified and DNA markers were developed. We also found that the RF genes from Solanum pimpinellifolium and S.l.cerasiforme are loci for the same locus.

Academic Significance and Societal Importance of the Research Achievements

RF遺伝子の座乗領域を特定でき、稔性回復と強度に連鎖するDNAマーカーの開発に至った。この結果、迅速にRF遺伝子座が導入された花粉親の開発が可能である。現在、CMSを利用したF1採種システムはトマトで開発されていないが、このDNAマーカーを活用することで、高純度の種子生産が可能なF1採種システムの開発に繋がると期待される。