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Isolation and structure determination of chemotactic regulatory substances as basic technology for the development of new plant protection method.

Research Project

Project/Area Number 17H03812
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Natsume Masahiro  東京農工大学, (連合)農学研究科(研究院), 教授 (10201683)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Keywords青枯病 / 走化性 / トマト / 根滲出物
Outline of Final Research Achievements

A chemoattractant of Ralstonia solanacearum isolated from the activated charcoal-adsorbed fraction of tomato root exudates was identified as ethyl β-D-glucopyranoside by instrumental analyses and comparison with synthetic preparations. Ethyl β-D-glucopyranoside showed unambiguous activity at above 1 μmol/disc. Its stereoisomers and D-glucose were inactive.
The effect of secondary metabolites and plant hormones produced by tomato on the chemotaxis of R. solanacearum was studied. Several hydroxylated aromatic carboxylic acids and a plant hormone, jasmonic acid, showed chemoattractant activity. Salicylic acid showed both chemoattractant and repellent activity depending on the concentration.

Academic Significance and Societal Importance of the Research Achievements

細菌の走化性誘引物質に関する研究は古くから行われているが、従来は根滲出物の成分の走化性誘引活性を調べるにとどまっており、活性を指標に根滲出物から走化性誘引物質を単離構造決定した研究は本報告が初めてであり、その学術的意義は大きい。
トマトの二次代謝産物として報告されている水酸化された芳香族カルボン酸が走化性誘引活性や忌避活性を示すことを見出したことは、既知の走化性誘引物質がアミノ酸、有機酸や糖など水溶性物質であることを考えると、新しい発見であり、走化性誘引物質や忌避物質の農業への応用に新たな道を拓くものであると期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (10 results)

All 2019 2018 2017 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Effect of Secondary Metabolites of Tomato (Solanum lycopersicum) on Chemotaxis of Ralstonia solanacearum, Pathogen of Bacterial Wilt Disease2019

    • Author(s)
      Hasegawa Takuya、Kato Yusuke、Okabe Atsushi、Itoi Chie、Ooshiro Atsushi、Kawaide Hiroshi、Natsume Masahiro
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 67 Issue: 7 Pages: 1807-1813

    • DOI

      10.1021/acs.jafc.8b06245

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethyl β-d-glucoside: a novel chemoattractant of Ralstonia solanacearum isolated from tomato root exudates by a bioassay-guided fractionation2018

    • Author(s)
      Hasegawa Takuya、Okabe Atsushi、Kato Yusuke、Ooshiro Atsushi、Kawaide Hiroshi、Natsume Masahiro
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 12 Pages: 2049-2052

    • DOI

      10.1080/09168451.2018.1515618

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates2018

    • Author(s)
      Hasegawa Takuya、Okabe Atsushi、Kato Yusuke、Ooshiro Atsushi、Kawaide Hiroshi、Natsume Masahiro
    • Journal Title

      Journal of General Plant Pathology

      Volume: 84 Issue: 1 Pages: 20-26

    • DOI

      10.1007/s10327-017-0752-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] トマトが生産する二次代謝産物に対する青枯病菌の走化性応答2019

    • Author(s)
      長谷川 琢也, 井樋 千絵, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      平成31年度 日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 青枯病菌の走化性誘引物質の探索(第七報)2018

    • Author(s)
      長谷川 琢也, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      日本農薬学会第43回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 青枯病菌の走化性誘引物質の探索(第 5 報)2018

    • Author(s)
      長谷川 琢也, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青枯病菌の走化性誘引物質の探索(第6報)2018

    • Author(s)
      長谷川 琢也, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      平成30年度 日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青枯病菌の走化性誘引物質の探索(第3報)2017

    • Author(s)
      長谷川 琢也, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青枯病菌の走化性誘引物質の探索(第4報)2017

    • Author(s)
      長谷川 琢也, 岡部 淳, 加藤 雄亮, 川出 洋, 夏目 雅裕
    • Organizer
      日本農芸化学会関東支部2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 植物病原細菌の走化性誘引物質に関する研究

    • URL

      http://web.tuat.ac.jp/~chemreg/natsume2-2.html

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report

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Published: 2017-04-28   Modified: 2023-12-25  

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