Development of the novel theraphy for intractable preterm birth complications using amniotic fluid stem cells

• Project/Area Number: 17H04236
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Keio University
• Principal Investigator: Tanaka Mamoru 慶應義塾大学, 医学部(信濃町), 教授 (20207145)
• Co-Investigator(Kenkyū-buntansha): 落合 大吾 慶應義塾大学, 医学部(信濃町), 講師 (80348713) ; 升田 博隆 慶應義塾大学, 医学部(信濃町), 講師(非常勤) (80317198)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: ヒト羊水幹細胞 / 間葉系幹細胞 / 早産 / 敗血症 / 抗炎症作用 / 脳性麻痺 / 再生医療 / 幹細胞治療 / ニューロスフェア / 未熟児医学 / 幹細胞 / 羊水

Outline of Final Research Achievements

In Japan, there are 50,000 preterm deliveries per year, about 40% of which are caused by intrauterine inflammation. The development of the novel therapy for preterm birth and its complications is an urgent issue in the perinatal field.
We injected lipopolysaccharide into the peritoneal cavity of P3 rats and created rat model of neonatal sepsis. We investigated the effect of human amniotic fluid stem (hAFSC) on the neonatal sepsis rat. The improvement in survival, reduction of proinflammatory cytokines in the blood, and inhibition of inflammatory multiple organ injuries were observed by hAFSC treatment only before lipopolysaccharide administration. Intraperitoneal cell aggregates, which contain hAFSC and macrophages, were assumed to contribute to the therapeutic effect.

Academic Significance and Societal Importance of the Research Achievements

早産合併症には現時点で有効な治療法がない．しかし，我々は本研究課題を通じて，胎児由来のヒト羊水幹細胞が早産合併症に治療効果を有する可能性を見出した．この治療効果はヒト羊水幹細胞の抗炎症作用に起因すると考えられ，生存率の改善という最大のエンドポイントを改善した点は今後の臨床応用に向けて重要な結果であると考える．
また，治療機序に関する検討も進んでおり，抗炎症作用や細胞凝集体の寄与などといった，これまで明らかになっていないヒト羊水幹細胞による炎症性疾患の治療機序の解明に繋がる可能性がある．

Research Products

• [Journal Article] In Utero Amniotic Fluid Stem Cell Therapy Protects Against Myelomeningocele via Spinal Cord Coverage and Hepatocyte Growth Factor Secretion (2019)
  • Author(s): Abe Yushi、Ochiai Daigo、Masuda Hirotaka、Sato Yu、Otani Toshimitsu、Fukutake Marie、Ikenoue Satoru、Miyakoshi Kei、Okano Hideyuki、Tanaka Mamoru
  • Journal Title: STEM CELLS Translational Medicine Volume: 8 Issue: 11 Pages: 1170-1179
  • DOI: 10.1002/sctm.19-0002
  • Peer Reviewed / Open Access
• [Journal Article] Glycemic and metabolic features in gestational diabetes: singleton <i>versus</i> twin pregnancies (2019)
  • Author(s): Akiba Y, Miyakoshi K, Ikenoue S, Saisho Y, Kasuga Y, Ochiai D, Matsumoto T, Tanaka M.
  • Journal Title: Endocrine Journal Volume: 66 Issue: 7 Pages: 647-651
  • DOI: 10.1507/endocrj.EJ18-0575
  • NAID: 130007684328
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access
• [Journal Article] Human amniotic fluid stem cells have a unique potential to accelerate cutaneous wound healing with reduced fibrotic scarring like a fetus. (2019)
  • Author(s): Fukutake M, Ochiai D, Masuda H, Abe Y, Sato Y, Otani T, Sakai S, Aramaki-Hattori N, Shimoda M, Matsumoto T, Miyakoshi K, Kanai Y, Kishi K, Tanaka M.
  • Journal Title: Hum Cell. Volume: 32 Issue: 1 Pages: 51-63
  • DOI: 10.1007/s13577-018-0222-1
  • Peer Reviewed
• [Journal Article] Ultrasound Findings of Varices at the Site of Uterovaginal Anastomosis in a Pregnant Woman After Radical Trachelectomy (2019)
  • Author(s): Kasuga Yoshifumi、Miyakoshi Kei、Tanaka Mamoru
  • Journal Title: Journal of Ultrasound in Medicine Volume: 38 Issue: 12 Pages: 3363-3364
  • DOI: 10.1002/jum.15034
  • Peer Reviewed
• [Journal Article] Transvaginal ultrasound features of the residual cervix in pregnancy after radical trachelectomy. (2019)
  • Author(s): Kasuga Yoshifumi、Miyakoshi Kei、Tanaka Mamoru
  • Journal Title: Journal of Medical Ultrasonics Volume: － Issue: 2 Pages: 335-337
  • DOI: 10.1007/s10396-020-01005-x
  • Peer Reviewed
• [Journal Article] National survey of uterine rupture in Japan: Annual report of Perinatology Committee, Japan Society of Obstetrics and Gynecology, 2018. (2019)
  • Author(s): Makino S, Takeda S, Kondoh E, Kawai K, Takeda J, Matsubara S, Itakura A, Sago H, Tanigaki S, Tanaka M, Ikeda T, Kanayama N.
  • Journal Title: J Obstet Gynaecol Res. Volume: 45 Issue: 4 Pages: 763-765
  • DOI: 10.1111/jog.13924
  • Peer Reviewed
• [Journal Article] The orientation of a decellularized uterine scaffold determines the tissue topology and architecture of the regenerated uterus in rats (2019)
  • Author(s): Miki Fumie、Maruyama Tetsuo、Miyazaki Kaoru、Takao Tomoka、Yoshimasa Yushi、Katakura Satomi、Hihara Hanako、Uchida Sayaka、Masuda Hirotaka、Uchida Hiroshi、Nagai Toshihiro、Shibata Shinsuke、Tanaka Mamoru
  • Journal Title: Biology of Reproduction Volume: - Issue: 5 Pages: 1215-1227
  • DOI: 10.1093/biolre/ioz004
  • Peer Reviewed / Open Access
• [Journal Article] Risk of preterm birth after the excisional surgery for cervical lesions: a propensity-score matching study in Japan (2019)
  • Author(s): Miyakoshi Kei、Itakura Atsuo、Abe Takayuki、Kondoh Eiji、Terao Yasuhisa、Tabata Tsutomu、Hamada Hiromi、Tanaka Kyoko、Tanaka Mamoru、Kanayama Naohiro、Takeda Satoru
  • Journal Title: The Journal of Maternal-Fetal & Neonatal Medicine Volume: － Issue: 6 Pages: 1-7
  • DOI: 10.1080/14767058.2019.1619687
  • Peer Reviewed
• [Journal Article] Changes in bone metabolic profile associated with pregnancy or lactation. (2019)
  • Author(s): Miyamoto T, Miyakoshi K, Sato Y, Kasuga Y, Ikenoue S, Miyamoto K, Nishiwaki Y, Tanaka M, Nakamura M, Matsumoto M.
  • Journal Title: Sci Rep. Volume: 9 Issue: 1 Pages: 6787-6787
  • DOI: 10.1038/s41598-019-43049-1
  • Peer Reviewed / Open Access
• [Journal Article] Zscan5b Deficiency Impairs DNA Damage Response and Causes Chromosomal Aberrations during Mitosis. (2019)
  • Author(s): Ogawa S, Yamada M, Nakamura A, Sugawara T, Nakamura A, Miyajima S, Harada Y, Ooka R, Okawa R, Miyauchi J, Tsumura H, Yoshimura Y, Miyado K, Akutsu H, Tanaka M, Umezawa A, Hamatani T.
  • Journal Title: Stem Cell Reports Volume: 12 Issue: 6 Pages: 1366-1379
  • DOI: 10.1016/j.stemcr.2019.05.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of Oxidative Stress on Age-Associated Decline in Oocyte Developmental Competence (2019)
  • Author(s): Sasaki Hiroyuki、Hamatani Toshio、Kamijo Shintaro、Iwai Maki、Kobanawa Masato、Ogawa Seiji、Miyado Kenji、Tanaka Mamoru
  • Journal Title: Frontiers in Endocrinology Volume: 10 Pages: 811-811
  • DOI: 10.3389/fendo.2019.00811
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation. (2019)
  • Author(s): Sasaki H, Kawamura K, Kawamura T, Odamaki T, Katsumata N, Xiao JZ, Suzuki N, Tanaka M.
  • Journal Title: Reprod Biomed Online. Volume: 38 Issue: 4 Pages: 570-578
  • DOI: 10.1016/j.rbmo.2018.12.026
  • Peer Reviewed / Open Access
• [Journal Article] 分娩後に急性腎不全を発症し非典型溶血性尿毒症症候群(aHUS)と診断した1例 (2019)
  • Author(s): 竹田将人，池ノ上学，坂井健良，遠藤豊英，大石真希，落合大吾，宮越敬，田中守，青木大輔
  • Journal Title: 東京産科婦人科学会会誌 Volume: 68 Pages: 339-343
  • Peer Reviewed / Open Access
• [Journal Article] Membrane protein CD9 is repositioned and released to enhance uterine function. (2019)
  • Author(s): Iwai M, Hamatani T, Nakamura A, Kawano N, Kanai S, Kang W, Yoshii N, Odawara Y, Yamada M, Miyamoto Y, Saito T, Saito H, Miyado M, Umezawa A, Miyado K, Tanaka M.
  • Journal Title: Laboratory Investigation Volume: 99 Issue: 2 Pages: 200-209
  • DOI: 10.1038/s41374-018-0145-1
  • Peer Reviewed / Open Access
• [Journal Article] The neurorestorative effect of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic-ischemic encephalopathy in mice. (2019)
  • Author(s): Otani T, Ochiai D, Masuda H, Abe Y, Fukutake M, Matsumoto T, Miyakoshi K, Tanaka M.
  • Journal Title: Pediatr Res. Volume: 85 Issue: 1 Pages: 97-104
  • DOI: 10.1038/s41390-018-0131-8
  • Peer Reviewed
• [Journal Article] Safe and easy technique for the laparoscopic application of Seprafilm in gynecologic surgery. (2018)
  • Author(s): Ota K, Sato K, Ogasawara J, Takahashi T, Mizunuma H, Tanaka M.
  • Journal Title: Asian J Endosc Surg. Volume: 印刷中 Issue: 2 Pages: 242-245
  • DOI: 10.1111/ases.12621
  • Peer Reviewed / Open Access
• [Journal Article] Impaired fetal growth in mothers with inadequate gestational weight gain: a retrospective study in Japanese uncomplicated pregnancy. (2018)
  • Author(s): Ikenoue S, Miyakoshi K, Kasuga Y, Ochiai D, Matsumoto T, Tanaka M.
  • Journal Title: J Matern Fetal Neonatal Med. Volume: 2 Issue: 13 Pages: 1-5
  • DOI: 10.1080/14767058.2018.1545835
  • Peer Reviewed
• [Journal Article] Chemotactic behavior of egg mitochondria in response to sperm fusion in mice (2018)
  • Author(s): Iwai Maki、Harada Yuichirou、Miyabayashi Rinako、Kang Woojin、Nakamura Akihiro、Kawano Natsuko、Miyamoto Yoshitaka、Yamada Mitsutoshi、Hamatani Toshio、Miyado Mami、Yoshida Keiichi、Saito Hidekazu、Tanaka Mamoru、Umezawa Akihiro、Miyado Kenji
  • Journal Title: Heliyon Volume: 4 Issue: 11 Pages: e00944-e00944
  • DOI: 10.1016/j.heliyon.2018.e00944
  • Peer Reviewed / Open Access
• [Journal Article] Lower serum total bilirubin concentration is associated with higher prevalence of gestational diabetes mellitus in Japanese pregnant women (2018)
  • Author(s): Nishimura T, Tanaka M, Saisho Y, Miyakoshi K, Tanaka M, Itoh H.
  • Journal Title: Endocrine Journal Volume: 65 Issue: 12 Pages: 1199-1208
  • DOI: 10.1507/endocrj.EJ17-0533
  • NAID: 130007553320
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Clinical and genetic characteristics of abnormal glucose tolerance in Japanese women in the first year after gestational diabetes mellitus. (2018)
  • Author(s): Kasuga Y, Miyakoshi K, Tajima A, Saisho Y, Ikenoue S, Ochiai D, Matsumoto T, Arata N, Hata K, Tanaka M.
  • Journal Title: J Diabetes Investig. Volume: 印刷中 Issue: 3 Pages: 817-826
  • DOI: 10.1111/jdi.12935
  • Peer Reviewed / Open Access
• [Journal Article] Degradation of phosphate polymer polyP enhances lactic fermentation in mice. (2018)
  • Author(s): Nakamura A, Kawano N, Motomura K, Kuroda A, Sekiguchi K, Miyado M, Woojin K, Miyamoto Y, Hanai M, Iwai M, Yamada M, Hamatani T, Saito T, Saito H, Tanaka M, Umezawa A, Miyado K.
  • Journal Title: Genes Cells. Volume: 23(10) Issue: 10 Pages: 904-914
  • DOI: 10.1111/gtc.12639
  • Peer Reviewed / Open Access
• [Journal Article] Human Amniotic Fluid Stem Cells: Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease (2018)
  • Author(s): Ochiai D, Masuda H, Abe Y, Otani T, Fukutake M, Matsumoto T, Miyakoshi K, Tanaka M
  • Journal Title: The Keio Journal of Medicine Volume: 67 Issue: 4 Pages: 57-66
  • DOI: 10.2302/kjm.2017-0019-IR
  • NAID: 130007552319
  • ISSN: 0022-9717, 1880-1293
  • Peer Reviewed
• [Journal Article] Association of Maternal Factors with Perinatal Complications in Pregnancies Complicated with Diabetes: A Single-Center Retrospective Analysis. (2018)
  • Author(s): Endo S, Saisho Y, Miyakoshi K, Ochiai D, Matsumoto T, Kawano Y, Mitsuishi M, Irie J, Tanaka M, Meguro S, Tanaka M, Itoh H.
  • Journal Title: J Clin Med Volume: 7 Issue: 1 Pages: 5-5
  • DOI: 10.3390/jcm7010005
  • Peer Reviewed / Open Access
• [Journal Article] Retrospective multicenter study of leaving the placenta in situ for patients with placenta previa on a cesarean scar. (2018)
  • Author(s): Miyakoshi K, Otani T, Kondoh E, Makino S, Tanaka M, Takeda S; Perinatal Research Network Group in Japan..
  • Journal Title: Int J Gynaecol Obstet. Volume: 140 Issue: 3 Pages: 345-351
  • DOI: 10.1002/ijgo.12397
  • Peer Reviewed
• [Journal Article] 羊水幹細胞を用いた胎児治療（現状と将来像） (2018)
  • Author(s): 落合大吾, 田中 守
  • Journal Title: 産科と婦人科 Volume: 85 Pages: 1085-1088
  • Peer Reviewed
• [Journal Article] 妊娠中の母体体重増加不良と出生体重に関する後方視的検討 (2018)
  • Author(s): 池ノ上 学, 宮越 敬, 佐藤 佑, 秋葉 洋平, 大谷 利光, 福武 麻里絵, 春日 義史, 落合 大吾, 松本 直, 田中 守
  • Journal Title: 糖尿病と妊娠 Volume: 18
  • Peer Reviewed
• [Journal Article] 多胎妊娠と糖代謝異常より良い管理を目指して 双胎妊娠における妊娠糖尿病の糖代謝異常重症度に関する検討 (2018)
  • Author(s): 秋葉 洋平, 宮越 敬, 税所 芳史, 佐藤 佑, 池ノ上 学, 落合 大吾, 松本 直, 田中 守
  • Journal Title: 糖尿病と妊娠 Volume: 18
  • Peer Reviewed
• [Journal Article] 妊娠糖尿病管理中の低カリウム性四肢麻痺を契機に検出されたCushing症候群合併妊娠の1例 (2018)
  • Author(s): 高橋 美央, 秋葉 洋平, 宮越 敬, 安康 真由香, 今枝 慶蓉, 落合 大吾, 松本 直, 田中 守, 青木 大輔
  • Journal Title: 東京産科婦人科学会会誌 Volume: 67 Pages: 280-283
  • Peer Reviewed
• [Journal Article] Human Amniotic Fluid Stem Cells: Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease. (2018)
  • Author(s): Ochiai D, Masuda H, Abe Y, Otani T, Fukutake M, Matsumoto T, Miyakoshi K, Tanaka M.
  • Journal Title: Keio J Med Volume: 印刷中
  • NAID: 130007552319
  • Peer Reviewed
• [Journal Article] Umbilical cord hemangioma: Sonographic features by HDlive Flow. (2018)
  • Author(s): Akiba Y, Miyakoshi K, Ochiai D, Kawaida M, Matsumoto T, Tanaka M.
  • Journal Title: Eur J Obstet Gynecol Reprod Biol. Volume: 221 Pages: 195-196
  • Peer Reviewed
• [Journal Article] Discordant fetal phenotype of hypophosphatasia in two siblings. (2018)
  • Author(s): Ikenoue S, Miyakoshi K, Ishii T, Sato Y, Otani T, Akiba Y, Kasuga Y, Ochiai D, Matsumoto T, Ichihashi Y, Matsuzaki Y, Tachikawa K, Michigami T, Nishimura G, Ikeda K, Hasegawa T, Tanaka M
  • Journal Title: American journal of medical genetics. Part A Volume: 176 Issue: 1 Pages: 171-174
  • DOI: 10.1002/ajmg.a.38531
  • Peer Reviewed
• [Journal Article] Laparoscopic fenestration of a large peritoneal inclusion cyst: Description of a new surgical technique. (2017)
  • Author(s): Sato K, Ota K, Maki J, Sato S, Masuda H, Tanaka M.
  • Journal Title: Eur J Obstet Gynecol Reprod Biol. Volume: 219 Pages: 68-69
  • Peer Reviewed
• [Journal Article] Impact of borderline-subclinical hypothyroidism on subsequent pregnancy outcome in women with unexplained recurrent pregnancy loss (2017)
  • Author(s): Uchida S, Maruyama T, Kagami M, Miki F, Hihara H, Katakura S, Yoshimasa Y, Masuda H, Uchida H, Tanaka M
  • Journal Title: J Obstet Gynaecol Res Volume: 43 Issue: 6 Pages: 1014-1020
  • DOI: 10.1111/jog.13319
  • Peer Reviewed
• [Journal Article] ZEB1 expression is a potential indicator of invasive endometriosis (2017)
  • Author(s): Furuya M, Masuda H, Hara K, Uchida H, Sato K, Sato S, Asada H, Maruyama T, Yoshimura Y, Katabuchi H, Tanaka M, Saya H
  • Journal Title: Acta Obstet Gynecol Scand Volume: 96 Issue: 9 Pages: 1128-1135
  • DOI: 10.1111/aogs.13179
  • Peer Reviewed
• [Journal Article] Mid-trimester residual cervical length and the risk of preterm birth in pregnancies after abdominal radical trachelectomy: a retrospective analysis. (2017)
  • Author(s): Kasuga Y, Miyakoshi K, Nishio H, Akiba Y, Otani T, Fukutake M, Ikenoue S, Ochiai D, Matsumoto T, Tanaka K, Minegishi K, Kuji N, Roberts R, Aoki D, Tanaka M.
  • Journal Title: BJOG. Volume: 印刷中 Issue: 11 Pages: 1729-1735
  • DOI: 10.1111/1471-0528.14688
  • Peer Reviewed
• [Journal Article] Association of common polymorphisms with gestational diabetes mellitus in Japanese women: A case-control study (2017)
  • Author(s): Kasuga, Y. Hata, K. Tajima, A. Ochiai, D. Saisho, Y. Matsumoto, T. Arata, N. Miyakoshi, K. Tanaka, M.
  • Journal Title: Endocrine Journal Volume: 64 Issue: 4 Pages: 463-475
  • DOI: 10.1507/endocrj.EJ16-0431
  • NAID: 130005630406
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low dose resveratrol ameliorates mitochondrial respiratory dysfunction and enhances cellular reprogramming. (2017)
  • Author(s): Mizuguchi Y, Hatakeyama H, Sueoka K, Tanaka M, Goto YI.
  • Journal Title: Mitochondrion. Volume: 34 Pages: 43-48
  • Peer Reviewed
• [Journal Article] Prenatal sonographic images of left pulmonary artery sling. (2017)
  • Author(s): Ochiai D, Miyakoshi K, Koinuma G, Matsumoto T, Tanaka M.
  • Journal Title: Eur J Obstet Gynecol Reprod Biol. Volume: 211 Pages: 217-218
  • DOI: 10.1016/j.ejogrb.2016.12.026
  • Peer Reviewed
• [Presentation] The therapeutic potentials of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic-ischemic encephalopathy (2019)
  • Author(s): Otani T，Ochiai D，Abe Y，Endo T，Oishi M，Sato Y，Fukutake M，Ikenoue S，Masuda H，Miyakoshi K，Tanaka M，Aoki D
  • Organizer: 第71回日本産科婦人科学会
• [Presentation] 三次元スフェア培養はヒト羊水幹細胞の抗炎症性サイトカイン分泌と未分化マーカー発現を増強する (2019)
  • Author(s): 佐藤佑，落合大吾，阿部雄志，遠藤豊英，大石真希，大谷利光，福武麻里絵，池ノ上学，升田博隆，宮越敬，田中守，青木大輔
  • Organizer: 第71回日本産科婦人科学会
• [Presentation] ヒト羊水幹細胞投与はLPS誘発性新生仔炎症モデルラットの予後を改善する (2019)
  • Author(s): 佐藤佑，落合大吾，大谷利光，福武麻里絵，池ノ上学，宮越敬，田中守
  • Organizer: 第55回日本周産期・新生児医学会
• [Presentation] A novel strategy to treat fetal myelomeningocele using human amniotic fluid stem cells (2019)
  • Author(s): Sato Y，Ochiai D，Abe Y，OtaniT，Fukutake M，Ikenoue S，Masuda H，Miyakoshi K，Tanaka M
  • Organizer: RCOG World Congress 2019
  • Int'l Joint Research
• [Presentation] Mirror症候群に急激な血小板減少を伴った1例 (2019)
  • Author(s): 遠藤豊英，池ノ上学，大石真希，落合大吾，宮越敬，田中守
  • Organizer: 第55回日本周産期・新生児医学会
• [Presentation] 子宮内膜異型増殖症および早期子宮体癌に対する高用量黄体ホルモン療法後妊娠の産科的予後に関する検討 (2019)
  • Author(s): 大石真希，宮越敬，遠藤豊英，池ノ上学，落合大吾，田中守
  • Organizer: 第55回日本周産期・新生児医学会
• [Presentation] 性分化疾患を含む多発先天異常を胎内診断し周産期管理を行った1例 (2019)
  • Author(s): 明石一浩，池ノ上学，遠藤豊英，大石真希，落合大吾，宮越敬，田中守，長谷川奉延，飛彈麻里子，有光威志，高橋萌，若林大樹，玉岡哲，黒田達夫
  • Organizer: 第55回日本周産期・新生児医学会
• [Presentation] A POTENTIAL THERAPY FOR FETAL MYELOMENINGOCELE BY HUMAN AMNIOTIC FLUID STEM CELLS. (2018)
  • Author(s): Abe Y, Ochiai D, Masuda H, Sato Y, Otani T, Fukutake M, Tanaka M
  • Organizer: International Society for Stem Cell Research (ISSCR 2018)
  • Int'l Joint Research
• [Presentation] The therapeutic potential of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic-ischemic encephalopathy in mice (2018)
  • Author(s): Otani T, Ochiai D, Masuda H, Fukutake M, Sato Y, Ikenoue S, Miyakoshi K, Tanaka M
  • Organizer: 8th Korea-Taiwan-Japan Symposium on Maternal-Fetal Medicine
  • Int'l Joint Research
• [Presentation] THE THERAPEUTIC EFFECTS OF HUMAN AMNIOTIC FLUID STEM CELLS ON THE CHRONIC PHASE OF NEONATAL HYPOXIC-ISCHEMIC ENCEPHALOPATHY (2018)
  • Author(s): Otani T, Ochiai D, Masuda H, Fukutake M, Sato Y, Ikenoue S, Miyakoshi K, Tanaka M
  • Organizer: 20th Congress of the Federation of Asia and Oceania Perinatal Societies (FAOPS 2018)
  • Int'l Joint Research
• [Presentation] A NOVEL STRATEGY TO TREAT FETAL MYELOMENINGOCELE USING HUMAN AMNIOTIC FLUID STEM CELLS (2018)
  • Author(s): Sato Y, Ochiai D, Abe Y, OtaniT, Fukutake M, Ikenoue S, Masuda H, Miyakoshi K, Tanaka M
  • Organizer: 20th Congress of the Federation of Asia and Oceania Perinatal Societies (FAOPS 2018)
  • Int'l Joint Research
• [Presentation] Pregnancy Outcomes After Abdominal Radical Trachelectomy for Early-Stage Cervical Cancer (2018)
  • Author(s): Ochiai D, Tanaka M
  • Organizer: 2018 Kangwon-Keio International symposium
  • Int'l Joint Research / Invited
• [Presentation] Human Amniotic Fluid Stem Cells: Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease (2018)
  • Author(s): Sato Y, Tanaka M
  • Organizer: 2018 Kangwon-Keio International symposium
  • Int'l Joint Research / Invited
• [Presentation] ラット胎仔脊髄髄膜瘤モデルを使用したヒト羊水幹細胞の羊水腔内注入による胎児治療効果について (2018)
  • Author(s): 阿部雄志, 落合大吾, 大谷利光, 福武麻里絵, 佐藤 佑, 秋葉洋平, 松本 直, 升田博隆, 宮越 敬, 田中 守, 青木大輔
  • Organizer: 第70回 日本産科婦人科学会
• [Presentation] 新生仔低酸素虚血性脳症モデルマウスに対するヒト羊水幹細胞鼻腔内投与の治療効果に関する検討 (2018)
  • Author(s): 大谷利光, 落合大吾, 阿部雄志, 福武 麻里絵, 佐藤 佑, 秋葉洋平, 松本 直, 升田博隆, 宮越 敬, 田中 守, 青木大輔
  • Organizer: 第70回 日本産科婦人科学会
• [Presentation] 新生仔低酸素虚血性脳症モデルマウスに対するヒト羊水幹細胞投与の治療効果に関する検討 (2018)
  • Author(s): 大谷利光, 落合大吾, 佐藤 佑, 福武 麻里絵, 松本 直, 宮越 敬, 田中 守
  • Organizer: 第54回 日本周産期・新生児医学会
• [Presentation] A novel strategy to treat fetal myelomeningocele using human amniotic fluid stem cells (2018)
  • Author(s): Yushi Abe, Daigo Ochiai, Hirotaka Masuda, Toshimitsu Otani, Marie Fukutake, Youhei Akiba, Tadashi Matsumoto, Kei Miyakoshi, Mamoru Tanaka
  • Organizer: Society for Reproductive Investigation
  • Int'l Joint Research
• [Presentation] Human amniotic fluid stem cells have a unique potential to accelerate cutaneous wound healing with less fibrotic scarring like fetal wound healing (2018)
  • Author(s): Yu Sato, Daigo Ochiai, Hirotaka Masuda, Yushi Abe, Toshimitsu Otani, Marie Fukutake, Youhei Akiba, Tadashi Matsumoto, Kei Miyakoshi, Mamoru Tanaka
  • Organizer: Society for Reproductive Investigation
  • Int'l Joint Research
• [Presentation] Human amniotic fluid stem cells :A novel treatment option for neonatal cerebral palsy (2018)
  • Author(s): Toshimitsu Otani, Daigo Ochiai, Marie Fukutake, Yushi Abe, Yu Sato, Youhei Akiba, Tadashi Matsumoto, Hirotaka Masuda, Kei Miyakoshi, Mamoru Tanaka
  • Organizer: Society for Reproductive Investigation
  • Int'l Joint Research
• [Presentation] ヒト羊水幹細胞を用いた脊髄髄膜瘤に対する新規胎児治療法の開発 (2018)
  • Author(s): 阿部雄志，落合大吾，升田博隆，大谷利光，福武麻里絵，秋葉洋平，松本直，宮越敬，田中守
  • Organizer: 日本再生医療学会
• [Presentation] ヒト羊水幹細胞の投与前調整法に関する検討 (2017)
  • Author(s): 阿部雄志，落合大吾，福武麻里絵，大谷利光，升田博隆，秋葉洋平，松本直，宮越敬，田中守
  • Organizer: 臍帯血による再生医療研究会
• [Presentation] ヒト羊水幹細胞の羊水腔内注入によるラット脊髄髄膜瘤の治療効果に関する検討 (2017)
  • Author(s): 落合大吾, 阿部雄志, 大谷利光, 福武麻里絵, 升田博隆, 佐藤佑, 秋葉洋平, 松本直, 宮越敬, 田中守
  • Organizer: 日本胎児治療学会
• [Presentation] ヒト羊水幹細胞がマウス皮膚再生に与える影響 － 細胞局所注射ならびに細胞シート貼付における検討 (2017)
  • Author(s): 福武麻里絵, 落合大吾, 佐藤佑, 秋葉洋平, 大谷利光, 池ノ上学, 春日義史, 松本直, 宮越敬, 田中守
  • Organizer: 日本周産期・新生児医学会
• [Presentation] Effects of human amniotic fluid stem cells on cutaneous wound healing in a mouse excisional model (2017)
  • Author(s): Marie Fukutake, Daigo Ochiai, Hirotaka Masuda, Yu Sato, Youhei Akiba, Toshimitsu Otani, Satoru Ikenoue, Yoshifumi Kasuga, Tadashi Matsumoto, Kei Miyakoshi, Mamoru Tanaka, Daisuke Aoki
  • Organizer: Japan Society of Obstetrics and Gynecology
  • Int'l Joint Research
• [Presentation] Amniotic fluid derived Mesenchymal Stem Cells: Design for the future (2017)
  • Author(s): Mamoru Tanaka
  • Organizer: Korean Society of Perinatology
  • Int'l Joint Research / Invited