Molecular analyses of the mechanisms of severe pneumococcal pneumonia and search for control method using neutrophil immunity
Project/Area Number |
17H04367
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
|
Research Institution | Niigata University |
Principal Investigator |
Terao Yutaka 新潟大学, 医歯学系, 教授 (50397717)
|
Co-Investigator(Kenkyū-buntansha) |
土門 久哲 新潟大学, 医歯学系, 准教授 (00594350)
前川 知樹 新潟大学, 医歯学系, 研究教授 (50625168)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
|
Keywords | 好中球 / 肺炎球菌 / 肺炎 / 口腔細菌学 / 誤嚥性肺炎 |
Outline of Final Research Achievements |
It has long been known that pneumococci perform autolysis. In addition, recent pathological diagnosis has revealed that a large amount of neutrophils infiltrate human pneumococcal infection sites. Furthermore, it has been reported that the infectious disease specialists in Japan and overseas can reduce the severity of pneumonia by administering an inhibitor of neutrophil endogenous protease. However, there was no research paper that integrated and elucidated them. Therefore, we carried out the mass spectrometric analysis on the molecular mechanism by which pneumonia becomes severe from two directions: the pneumococcal side and the human neutrophil side. Then, we integrated the elucidation data of the pneumococcal side and the human side, and identified a group of molecular candidates involved in the severity of pneumonia
|
Academic Significance and Societal Importance of the Research Achievements |
誤嚥性肺炎を含めた肺炎は,耐性菌の出現と高齢社会を迎え増加・重症化している.2016年以降,日本政府および国連が,具体名を挙げて肺炎を人類の最脅威のひとつに定義している.WHOならびに国連の最新試算では,抗菌薬以外の対策を考案できなければ,2050年以降は毎年1000万人の死者が出ると予測されている.本研究の結果,肺炎球菌だけが好中球を敢えて周囲に集積させて好中球を傷害し,同細胞の内容物を利用して感染拡大を行うユニークな知見を掴んだ.今後は本研究成果に加え,好中球を利用する肺炎球菌の病原機構を詳細解析し,その制御法へと繋げることができる.
|
Report
(4 results)
Research Products
(92 results)
-
-
-
-
-
-
-
-
-
-
[Journal Article] A peptide derived from rice inhibits alveolar bone resorption via suppression of inflammatory cytokine production.2019
Author(s)
Aoki-Nonaka Y, Tabeta K, Yokoji M, Matsugishi A, Matsuda Y, Takahashi N, Sulijaya B, Domon H, Terao Y, Taniguchi M, Yamazaki K
-
Journal Title
J Periodontol
Volume: 90(10)
Pages: 1160-1169
Related Report
Peer Reviewed
-
-
[Journal Article] Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis.2019
Author(s)
Hiyoshi T, Domon H, Maekawa T, Nagai K, Tamura H, Takahashi N, Yonezawa D, Miyoshi T, Yoshida A, Tabeta, K, Terao Y
-
Journal Title
Microbiol Immunol
Volume: 63
Pages: 100-110
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Peptides from rice endosperm protein restrain periodontal bone loss in mouse model of periodontitis.2019
Author(s)
Tamura H, Maekawa T, Domon H, Hiyoshi T, Yonezawa D, Nagai K, Ochiai A, Taniguchi M, Tabeta K, Maeda T, Terao Y
-
Journal Title
Arch Oral Biol
Volume: 98
Pages: 132-139
Related Report
Peer Reviewed
-
-
[Journal Article] Antibacterial activity of hinokitiol against both antibiotic-resistant and -susceptible pathogenic bacteria predominant in the oral cavity and upper airways.2019
Author(s)
Domon, H., Hiyoshi, T., Maekawa, T., Yonezawa, D., Tamura, H., Kawabata, S., Yanagihara, K., Kimura, O., Kunitomo, E., and Terao, Y.
-
Journal Title
Microbiol. Immunol.
Volume: 印刷中
Related Report
Peer Reviewed
-
[Journal Article] Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis.2019
Author(s)
Hiyoshi, T., Domon, H., Maekawa, T., Nagai, K., Tamura, H., Takahashi, N., Yonezawa, D., Miyoshi, T., Yoshida, A., Tabeta, K., and Terao, Y.
-
Journal Title
Microbiol. Immunol.
Volume: 印刷中
NAID
Related Report
Peer Reviewed
-
[Journal Article] The AmyI-1-18 peptide derived from rice inhibits alveolar bone resorption via suppression of inflammatory cytokine production induced by lipopolysaccharide and interleukin-1beta.2019
Author(s)
Aoki-Nonaka, Y., Tabeta, K., Yokoji, M., Matsugishi, A., Matsuda, Y., Takahashi, N., Sulijaya, B., Domon, H., Terao, Y., Taniguchi, M., and Yamazaki, K.
-
Journal Title
J. Periodontol.
Volume: 印刷中
Related Report
Peer Reviewed
-
[Journal Article] Antimicrobial susceptibility of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis clinical isolates from children with acute otitis media in Japan from 2014 to 2017.2019
Author(s)
Nagai, K., Kimura, O., Domon, H., Maekawa, T., Yonezawa, D., and Terao, Y.
-
Journal Title
J. Infect. Chemother.
Volume: 25(3)
Pages: 229-232
Related Report
Peer Reviewed
-
[Journal Article] Peptides from rice endosperm protein restrain periodontal bone loss in mouse model of periodontitis.2019
Author(s)
Tamura, H., Maekawa, T., Domon, H., Hiyoshi, T., Yonezawa, D., Nagai, K., Ochiai, A., Taniguchi, M., Tabeta, K., Maeda, T., and Terao, Y.
-
Journal Title
Arch. Oral Biol.
Volume: 98
Pages: 132-139
Related Report
Peer Reviewed
-
[Journal Article] Repeated human deciduous tooth-derived dental pulp cell reprogramming factor transfection yields multipotent intermediate cells with enhanced iPS cell formation capability.2019
Author(s)
Soda, M., Saitoh, I., Murakami, T., Inada, E., Iwase, Y., Noguchi, H., Shibasaki, S., Kurosawa, M., Sawami, T., Terunuma, M., Kubota, N., Terao, Y., Ohshima, H., Hayasaki, H., and Sato, M.
-
Journal Title
Scientific Reports
Volume: 9
Pages: 1490-1490
Related Report
Peer Reviewed
-
[Journal Article] Mechanism of macrolide-induced inhibition of pneumolysin release involves impairment of autolysin release in macrolide-resistant Streptococcus pneumoniae.2018
Author(s)
Domon, H., Maekawa, T., Yonezawa, D., Nagai, K., Oda, M., Yanagihara, K., and Terao, Y.
-
Journal Title
Antimicrob. Agents Chemother.
Volume: 62(11)
Related Report
Peer Reviewed
-
[Journal Article] Streptococcus pyogenes CAMP factor promotes calcium ion uptake in RAW264.7 cells.2018
Author(s)
Kurosawa, M., Oda, M., Domon, H., Isono, T., Nakamura, Y., Saitoh, I., Hayasaki, H., Yamaguchi, M., Kawabata, S., and Terao, Y.
-
Journal Title
Microbiol. Immunol.
Volume: 62
Pages: 617-623
NAID
Related Report
Peer Reviewed
-
[Journal Article] Neutrophil elastase subverts the immune response by cleaving toll-like receptors and cytokines in pneumococcal pneumonia.2018
Author(s)
Domon, H., Nagai, K., Maekawa, T., Oda, M., Yonezawa, D., Takeda, W., Hiyoshi, T., Tamura, H., Yamaguchi, M., Kawabata, S., and Terao, Y.
-
Journal Title
Front. Immunol.
Volume: Vol. 9
Related Report
Peer Reviewed
-
[Journal Article] Sulfated vizantin induces the formation of macrophage extracellular traps.2018
Author(s)
Oda, M., Kurosawa, M., Yamamoto, H., Domon, H., Kimura, T., Isono, T., Maekawa, T., Hayashi, N., Yamada, N., Furue, Y., Kai, D., and Terao, Y.
-
Journal Title
Microbiol. Immunol.
Volume: 62
Pages: 310-316
NAID
Related Report
Peer Reviewed
-
[Journal Article] The effect of chlorhexidine on dental calculus formation: an in vitro study2018
Author(s)
Sakaue, Y., Takenaka, S., Ohsumi, T., Domon, H., Terao, Y., and Noiri, Y.
-
Journal Title
BMC Oral Health
Volume: 18
Pages: 52-52
Related Report
Peer Reviewed
-
[Journal Article] treptococcal cysteine protease-mediated cleavage of desmogleins is involved in the pathogenesis of cutaneous infection.2018
Author(s)
Sumitomo, T., Mori, Y., Nakamura, Y., Honda-Ogawa, M., Nakagawa, S., Yamaguchi, M., Matsue, H., Terao, Y., Nakata, M., and Kawabata, S.
-
Journal Title
Front. Cell. Infect. Microbiol.
Volume: Vol. 8
Related Report
Peer Reviewed
-
[Journal Article] Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4.2018
Author(s)
Nagai, K., Domon, H., Maekawa, T., Oda, M., Hiyoshi, T., Tamura, H., Yonezawa, D., Arai, Y., Yokoji, M., Tabeta, K., Habuka, R., Saitoh, A., Yamaguchi, M., Kawabata, S., and Terao, Y.
-
Journal Title
Cell. Immunol.
Volume: 325
Pages: 14-22
Related Report
Peer Reviewed
-
[Journal Article] treptococcus pyogenes CAMP factor promotes bacterial adhesion and invasion in pharyngeal epithelial cells without serum via PI3K/Akt signaling pathway.2018
Author(s)
Kurosawa, M., Oda, M., Domon, H., Isono, T., Nakamura, Y., Saitoh, I., Hayasaki, H., Yamaguchi, M., Kawabata, S., and Terao, Y.
-
Journal Title
Microb. Infect.
Volume: 20
Pages: 9-18
Related Report
Peer Reviewed
-
[Journal Article] Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-like Receptors and Cytokines in Pneumococcal Pneumonia.2018
Author(s)
Domon H, Nagai K, Maekawa T, Oda M, Yonezawa D, Takeda W, Hiyoshi T, Tamura H, Yamaguchi M, Kawabata S, Terao Y
-
Journal Title
Front Immunol
Volume: 印刷中
Related Report
Peer Reviewed
-
[Journal Article] Sulfated vizantin induces the formation of macrophage extracellular traps.2018
Author(s)
Oda M, Kurosawa M, Yamamoto H, Domon H, Kimura T, Isono T, Maekawa T, Hayashi N, Yamada N, Furue Y, Kai D, Terao Y
-
Journal Title
Microbiol Immunol
Volume: 印刷中
NAID
Related Report
Peer Reviewed
-
-
-
[Journal Article] Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4.2018
Author(s)
Nagai K, Domon H., Maekawa T, Oda M, Hiyoshi T, Tamura H, Yonezawa D, Arai Y, Yokoji M, Tabeta K, Habuka R, Saitoh A, Yamaguchi M, Kawabata S, Terao Y
-
Journal Title
Cell Immunol
Volume: 325
Pages: 14-22
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers.2017
Author(s)
Murakami T, Saitoh I, Sato M, Inada E, Soda M, Oda M, Domon H, Iwase Y, Sawami T, Matsueda K, Terao Y, Ohshima H, Noguchi H, Hayasaki H
-
Journal Title
Arch Oral Biol
Volume: 81
Pages: 110-120
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Update on the Immune Mechanisms Against Respiratory Pathogens2019
Author(s)
Domon H, Nagai K, Maekawa T, Oda M, Yonezawa D, Takeda W, Hiyoshi T, Tamura H, Yamaguchi M, Kawabata S, Terao Y
Total Pages
355
Publisher
Frontiers e-book
Related Report
-
-
-
-
-
-
-
-